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Literature summary for 2.4.1.332 extracted from

  • Touhara, K.K.; Nihira, T.; Kitaoka, M.; Nakai1, H.; Fushinobu, S.
    Structural basis for reversible phosphorolysis and hydrolysis reactions of 2-O-alpha-glucosylglycerol phosphorylase (2014), J. Biol. Chem., 289, 18067-18075.
    View publication on PubMed
Search for more literature for 2.4.1.332

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Salisediminibacterium selenitireducens

Crystallization (Commentary)

Crystallization (Comment) Organism
obtained at 20ΒΊC using the hanging drop vapor diffusion method by mixing 1 microL of protein solution containing 4.3Β–5.7 mg/mL protein with an equal volume of reservoir solution containing 28% polyethylene glycol 400, 0.1 M HEPES-NaOH (pH 7.5), and 0.2 M CaCl2 Salisediminibacterium selenitireducens

Protein Variants

Protein Variants Comment Organism
E475Q inactive mutant Salisediminibacterium selenitireducens
K587A inactive mutant Salisediminibacterium selenitireducens
W381F inactive mutant Salisediminibacterium selenitireducens
Y327F inactive mutant Salisediminibacterium selenitireducens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-O-alpha-D-glucopyranosyl-sn-glycerol + phosphate Salisediminibacterium selenitireducens
-
 beta-D-glucose 1-phosphate + sn-glycerol
-
 r
2-O-alpha-D-glucopyranosyl-sn-glycerol + phosphate Salisediminibacterium selenitireducens DSM 15326
-
 beta-D-glucose 1-phosphate + sn-glycerol
-
 r
beta-D-glucose 1-phosphate + sn-glycerol Salisediminibacterium selenitireducens
-
 2-O-alpha-D-glucopyranosyl-sn-glycerol + phosphate
-
 r
beta-D-glucose 1-phosphate + sn-glycerol Salisediminibacterium selenitireducens DSM 15326
-
 2-O-alpha-D-glucopyranosyl-sn-glycerol + phosphate
-
 r

Organism

Organism UniProt Comment Textmining
Salisediminibacterium selenitireducens D6XZ22
-
-
Salisediminibacterium selenitireducens DSM 15326 D6XZ22
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-O-alpha-D-glucopyranosyl-sn-glycerol + phosphate
-
 Salisediminibacterium selenitireducens beta-D-glucose 1-phosphate + sn-glycerol
-
 r
2-O-alpha-D-glucopyranosyl-sn-glycerol + phosphate mechanism: an oxygen atom of phosphate attacks the anomeric C1 atom, and Glu475 works as a general acid Salisediminibacterium selenitireducens beta-D-glucose 1-phosphate + sn-glycerol
-
 r
2-O-alpha-D-glucopyranosyl-sn-glycerol + phosphate
-
 Salisediminibacterium selenitireducens DSM 15326 beta-D-glucose 1-phosphate + sn-glycerol
-
 r
2-O-alpha-D-glucopyranosyl-sn-glycerol + phosphate mechanism: an oxygen atom of phosphate attacks the anomeric C1 atom, and Glu475 works as a general acid Salisediminibacterium selenitireducens DSM 15326 beta-D-glucose 1-phosphate + sn-glycerol
-
 r
beta-D-glucose 1-phosphate + sn-glycerol
-
 Salisediminibacterium selenitireducens 2-O-alpha-D-glucopyranosyl-sn-glycerol + phosphate
-
 r
beta-D-glucose 1-phosphate + sn-glycerol
-
 Salisediminibacterium selenitireducens 2-O-alpha-D-glucopyranosyl-sn-glycerol + phosphate
-
 ?
beta-D-glucose 1-phosphate + sn-glycerol
-
 Salisediminibacterium selenitireducens DSM 15326 2-O-alpha-D-glucopyranosyl-sn-glycerol + phosphate
-
 r
beta-D-glucose 1-phosphate + sn-glycerol
-
 Salisediminibacterium selenitireducens DSM 15326 2-O-alpha-D-glucopyranosyl-sn-glycerol + phosphate
-
 ?
additional information in the absence of sn-glycerol the enzyme catalyzes the hydrolysis of beta-D-glucose 1-phosphate to alpha-D-glucose and phosphate. This reaction can be explained as a glycosyl transfer reaction to a water molecule Salisediminibacterium selenitireducens ?
-
 ?
additional information in the absence of sn-glycerol the enzyme catalyzes the hydrolysis of beta-D-glucose 1-phosphate to alpha-D-glucose and phosphate. This reaction can be explained as a glycosyl transfer reaction to a water molecule Salisediminibacterium selenitireducens DSM 15326 ?
-
 ?

Synonyms

Synonyms Comment Organism
2-O-alpha-D-glucopyranosylglycerol phosphorylase
-
 Salisediminibacterium selenitireducens
Bsel_2816 locus name Salisediminibacterium selenitireducens