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Literature summary for 2.4.1.330 extracted from

  • Nagatoshi, M.; Terasaka, K.; Owaki, M.; Sota, M.; Inukai, T.; Nagatsu, A.; Mizukami, H.
    UGT75L6 and UGT94E5 mediate sequential glucosylation of crocetin to crocin in Gardenia jasminoides (2012), FEBS Lett., 586, 1055-1061.
    View publication on PubMed
Search for more literature for 2.4.1.330

Inhibitors

Inhibitors Comment Organism Structure
beta-D-glucosyl crocetin above 0.2 mM, substrate inhibition Gardenia jasminoides
additional information no substrate inhibition with bis(beta-D-glucosyl) crocetin Gardenia jasminoides

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.023
-
 bis(beta-D-glucosyl) crocetin pH 7.5, 30°C Gardenia jasminoides
0.023
-
 bis(beta-D-glucosyl) crocetin pH not specified in the publication, temperature not specified in the publication Gardenia jasminoides
0.072
-
 beta-D-glucosyl crocetin pH 7.5, 30°C Gardenia jasminoides
0.072
-
 beta-D-glucosyl crocetin pH not specified in the publication, temperature not specified in the publication Gardenia jasminoides

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
49800
-
 calculated from sequence Gardenia jasminoides

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-alpha-D-glucose + beta-D-gentiobiosyl beta-D-glucosyl crocetin Gardenia jasminoides the enzyme is involved in biosynthesis of crocin UDP + crocin
-
 ?
UDP-alpha-D-glucose + beta-D-glucosyl crocetin Gardenia jasminoides the enzyme is involved in biosynthesis of crocin UDP + beta-D-gentiobiosyl crocetin
-
 ?
UDP-alpha-D-glucose + bis(beta-D-glucosyl) crocetin Gardenia jasminoides the enzyme is involved in biosynthesis of crocin UDP + beta-D-gentiobiosyl beta-D-glucosyl crocetin
-
 ?

Organism

Organism UniProt Comment Textmining
Gardenia jasminoides F8WKW8
-
-

Reaction

Reaction Comment Organism Reaction ID
UDP-alpha-D-glucose + beta-D-gentiobiosyl beta-D-glucosyl crocetin = UDP + crocin (3) Gardenia jasminoides
a
UDP-alpha-D-glucose + beta-D-glucosyl crocetin = UDP + beta-D-gentiobiosyl crocetin (1) Gardenia jasminoides
a
UDP-alpha-D-glucose + bis(beta-D-glucosyl) crocetin = UDP + beta-D-gentiobiosyl beta-D-glucosyl crocetin (2) Gardenia jasminoides
a

Source Tissue

Source Tissue Comment Organism Textmining
additional information mRNA expression is etected in all organs tested Gardenia jasminoides
-
seedling cultured cells obtained from seedlings Gardenia jasminoides
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no activity with crocetin Gardenia jasminoides ?
-
 ?
additional information enzyme does not show activity against various flavonoid glucosides and coumarin glucosides as well as unnatural glucosides such as curcumin glucosides and 4-nitrophenyl glucoside Gardenia jasminoides ?
-
 ?
UDP-alpha-D-glucose + beta-D-gentiobiosyl beta-D-glucosyl crocetin
-
 Gardenia jasminoides UDP + crocin
-
 ?
UDP-alpha-D-glucose + beta-D-gentiobiosyl beta-D-glucosyl crocetin the enzyme is involved in biosynthesis of crocin Gardenia jasminoides UDP + crocin
-
 ?
UDP-alpha-D-glucose + beta-D-glucosyl crocetin
-
 Gardenia jasminoides UDP + beta-D-gentiobiosyl crocetin
-
 ?
UDP-alpha-D-glucose + beta-D-glucosyl crocetin the enzyme is involved in biosynthesis of crocin Gardenia jasminoides UDP + beta-D-gentiobiosyl crocetin
-
 ?
UDP-alpha-D-glucose + bis(beta-D-glucosyl) crocetin
-
 Gardenia jasminoides UDP + beta-D-gentiobiosyl beta-D-glucosyl crocetin
-
 ?
UDP-alpha-D-glucose + bis(beta-D-glucosyl) crocetin the enzyme is involved in biosynthesis of crocin Gardenia jasminoides UDP + beta-D-gentiobiosyl beta-D-glucosyl crocetin
-
 ?

Synonyms

Synonyms Comment Organism
GjUGT9
-
 Gardenia jasminoides
UDP-glucose:crocetin glucosyl ester glucosyltransferase
-
 Gardenia jasminoides
UGT94E5
-
 Gardenia jasminoides

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Gardenia jasminoides

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.036
-
 beta-D-glucosyl crocetin pH 7.5, 30°C Gardenia jasminoides
0.036
-
 beta-D-glucosyl crocetin pH not specified in the publication, temperature not specified in the publication Gardenia jasminoides
0.071
-
 bis(beta-D-glucosyl) crocetin pH 7.5, 30°C Gardenia jasminoides
0.071
-
 bis(beta-D-glucosyl) crocetin pH not specified in the publication, temperature not specified in the publication Gardenia jasminoides

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Gardenia jasminoides

General Information

General Information Comment Organism
metabolism the enzyme is involved in biosynthesis of crocin Gardenia jasminoides
physiological function glycosyl transferase UGT94E5 is involved in synhtesis of apocarotenoid glycosyl ester crocin accumulating in fruits of Gardenia jasminoides and used as a food coloring and nutraceutical Gardenia jasminoides

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.51
-
 beta-D-glucosyl crocetin pH 7.5, 30°C Gardenia jasminoides
0.51
-
 beta-D-glucosyl crocetin pH not specified in the publication, temperature not specified in the publication Gardenia jasminoides
3.1
-
 bis(beta-D-glucosyl) crocetin pH 7.5, 30°C Gardenia jasminoides
3.1
-
 bis(beta-D-glucosyl) crocetin pH not specified in the publication, temperature not specified in the publication Gardenia jasminoides