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Literature summary for 2.4.1.323 extracted from

  • Asada, K.; Salim, V.; Masada-Atsumi, S.; Edmunds, E.; Nagatoshi, M.; Terasaka, K.; Mizukami, H.; de Luca, V.
    A 7-deoxyloganetic acid glucosyltransferase contributes a key step in secologanin biosynthesis in madagascar periwinkle (2013), Plant Cell, 25, 4123-4134.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.1.323

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Catharanthus roseus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.088
-
 UDP-alpha-D-glucose pH 8.0, 30°C Catharanthus roseus
5.38
-
 7-deoxyloganetate pH 8.0, 30°C Catharanthus roseus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-alpha-D-glucose + 7-deoxyloganetate Catharanthus roseus the enzyme is involved in loganin and secologanin biosynthesis UDP + 7-deoxyloganate
-
 ?

Organism

Organism UniProt Comment Textmining
Catharanthus roseus U3U992
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme, purified by nickel nitrilotriacetic acid affinity chromatography Catharanthus roseus

Source Tissue

Source Tissue Comment Organism Textmining
leaf preferentially expressed in internal phloem associated parenchyma cells Catharanthus roseus
-
phloem preferentially expressed in internal phloem associated parenchyma cells Catharanthus roseus
-
plant parenchyma preferentially expressed in internal phloem associated parenchyma cells Catharanthus roseus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-alpha-D-glucose + 7-deoxyloganetate the enzyme is involved in loganin and secologanin biosynthesis Catharanthus roseus UDP + 7-deoxyloganate
-
 ?
UDP-alpha-D-glucose + 7-deoxyloganetate does not react with 7-deoxyloganetin Catharanthus roseus UDP + 7-deoxyloganate
-
 ?

Synonyms

Synonyms Comment Organism
UGT8
-
 Catharanthus roseus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Catharanthus roseus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.13
-
 UDP-alpha-D-glucose pH 8.0, 30°C Catharanthus roseus
0.325
-
 7-deoxyloganetate pH 8.0, 30°C Catharanthus roseus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Catharanthus roseus

General Information

General Information Comment Organism
physiological function the enzyme is involved in loganin and secologanin biosynthesis Catharanthus roseus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.064
-
 7-deoxyloganetate pH 8.0, 30°C Catharanthus roseus
1.5
-
 UDP-alpha-D-glucose pH 8.0, 30°C Catharanthus roseus