Literature summary for 2.4.1.321 extracted from

Nihira, T.; Saito, Y.; Nishimoto, M.; Kitaoka, M.; Igarashi, K.; Ohtsubo, K.; Nakai, H.
Discovery of cellobionic acid phosphorylase in cellulolytic bacteria and fungi (2013), FEBS Lett., 587, 3556-3561.

Search for more literature for 2.4.1.321

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli	Xanthomonas campestris pv. campestris
expressed in Escherichia coli	Neurospora crassa
expression in Escherichia coli as a His-tagged fusion protein	Neurospora crassa
expression in Escherichia coli as a His-tagged fusion protein	Xanthomonas campestris

Inhibitors

Inhibitors	Comment	Organism	Structure
4-O-beta-D-glucopyranosyl-D-gluconate	-	Neurospora crassa

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism
0.1	-	phosphate	pH 7.0, 30°C	Neurospora crassa
0.21	-	alpha-D-glucose 1-phosphate	pH 6.5, 30°C	Xanthomonas campestris
0.21	-	alpha-D-glucose 1-phosphate	pH 6.0-6.5, 30°C	Xanthomonas campestris pv. campestris
1	-	4-O-beta-D-glucopyranosyl-D-gluconate	pH 7.0, 30°C	Neurospora crassa
2	-	D-gluconate	pH 6.5, 30°C	Xanthomonas campestris
2	-	D-gluconate	pH 6.0-6.5, 30°C	Xanthomonas campestris pv. campestris
2.4	-	D-glucuronate	pH 6.5, 30°C	Xanthomonas campestris
2.4	-	D-glucuronate	pH 6.0-6.5, 30°C	Xanthomonas campestris pv. campestris
3.4	-	D-gluconate	pH 6.5, 30°C	Neurospora crassa
3.4	-	D-gluconate	pH 6.0-6.5, 30°C	Neurospora crassa

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytoplasm	-	Xanthomonas campestris pv. campestris	5737	-
cytoplasm	-	Neurospora crassa	5737	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
84000	-	2 * 84000, SDS-PAGE	Xanthomonas campestris
84000	-	2 * 84000, SDS-PAGE, 2 * 89700, amino acid sequence	Xanthomonas campestris pv. campestris
87000	-	2 * 87000, SDS-PAGE	Neurospora crassa
87000	-	2 * 87000, SDS-PAGE, 2 * 90796, amino acid sequence	Neurospora crassa
89700	-	2 * 84000, SDS-PAGE, 2 * 89700, amino acid sequence	Xanthomonas campestris pv. campestris
89700	-	2 * 89700, calculated from sequence	Xanthomonas campestris
90769	-	2 * 90769, calculated from sequence	Neurospora crassa
90796	-	2 * 87000, SDS-PAGE, 2 * 90796, amino acid sequence	Neurospora crassa
171000	-	gel filtration	Neurospora crassa
179000	-	gel filtration	Xanthomonas campestris pv. campestris
179000	-	gel filtration	Xanthomonas campestris

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.
4-O-beta-D-glucopyranosyl-D-gluconate + phosphate	Neurospora crassa	-	alpha-D-glucose 1 phosphate + D-gluconate	-	r
4-O-beta-D-glucopyranosyl-D-gluconate + phosphate	Xanthomonas campestris pv. campestris	-	alpha-D-glucose 1-phosphate + D-gluconate	-	r
4-O-beta-D-glucopyranosyl-D-gluconate + phosphate	Neurospora crassa	it is suggested that the enzyme plays a crucial role in cellulose degradation where cellobionic acid as oxidized cellulolytic product is converted into alpha-D-glucose 1-phosphate and D-gluconic acid to enter glycolysis and the pentose phosphate pathway, respectively	alpha-D-glucose 1-phosphate + D-gluconate	-	r
4-O-beta-D-glucopyranosyl-D-gluconate + phosphate	Xanthomonas campestris	it is suggested that the enzyme plays a crucial role in cellulose degradation where cellobionic acid as oxidized cellulolytic product is converted into alpha-D-glucose 1-phosphate and D-gluconic acid to enter glycolysis and the pentose phosphate pathway, respectively	alpha-D-glucose 1-phosphate + D-gluconate	-	r
4-O-beta-D-glucopyranosyl-D-gluconate + phosphate	Neurospora crassa DSM 1257	-	alpha-D-glucose 1 phosphate + D-gluconate	-	r
4-O-beta-D-glucopyranosyl-D-gluconate + phosphate	Neurospora crassa DSM 1257	it is suggested that the enzyme plays a crucial role in cellulose degradation where cellobionic acid as oxidized cellulolytic product is converted into alpha-D-glucose 1-phosphate and D-gluconic acid to enter glycolysis and the pentose phosphate pathway, respectively	alpha-D-glucose 1-phosphate + D-gluconate	-	r
4-O-beta-D-glucopyranosyl-D-gluconate + phosphate	Xanthomonas campestris pv. campestris ATCC 33913	-	alpha-D-glucose 1-phosphate + D-gluconate	-	r
alpha-D-glucose 1 phosphate + D-gluconate	Neurospora crassa	-	4-O-beta-D-glucopyranosyl-D-gluconate + phosphate	-	r
alpha-D-glucose 1 phosphate + D-gluconate	Neurospora crassa DSM 1257	-	4-O-beta-D-glucopyranosyl-D-gluconate + phosphate	-	r
alpha-D-glucose 1-phosphate + D-gluconate	Xanthomonas campestris pv. campestris	-	4-O-beta-D-glucopyranosyl-D-gluconate + phosphate	-	r
alpha-D-glucose 1-phosphate + D-gluconate	Xanthomonas campestris pv. campestris ATCC 33913	-	4-O-beta-D-glucopyranosyl-D-gluconate + phosphate	-	r

Organism

Organism	UniProt	Comment	Textmining
Neurospora crassa	Q7S0S2	-	-
Neurospora crassa DSM 1257	Q7S0S2	-	-
Xanthomonas campestris	Q8P3J4	-	-
Xanthomonas campestris pv. campestris	Q8P3J4	-	-
Xanthomonas campestris pv. campestris ATCC 33913	Q8P3J4	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Xanthomonas campestris pv. campestris
-	Neurospora crassa
-	Xanthomonas campestris

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
4-O-beta-D-glucopyranosyl-D-gluconate + phosphate	-	Neurospora crassa	alpha-D-glucose 1 phosphate + D-gluconate	-	r
4-O-beta-D-glucopyranosyl-D-gluconate + phosphate	-	Neurospora crassa DSM 1257	alpha-D-glucose 1 phosphate + D-gluconate	-	r
4-O-beta-D-glucopyranosyl-D-gluconate + phosphate	-	Xanthomonas campestris pv. campestris	alpha-D-glucose 1-phosphate + D-gluconate	-	r
4-O-beta-D-glucopyranosyl-D-gluconate + phosphate	it is suggested that the enzyme plays a crucial role in cellulose degradation where cellobionic acid as oxidized cellulolytic product is converted into alpha-D-glucose 1-phosphate and D-gluconic acid to enter glycolysis and the pentose phosphate pathway, respectively	Neurospora crassa	alpha-D-glucose 1-phosphate + D-gluconate	-	r
4-O-beta-D-glucopyranosyl-D-gluconate + phosphate	it is suggested that the enzyme plays a crucial role in cellulose degradation where cellobionic acid as oxidized cellulolytic product is converted into alpha-D-glucose 1-phosphate and D-gluconic acid to enter glycolysis and the pentose phosphate pathway, respectively	Xanthomonas campestris	alpha-D-glucose 1-phosphate + D-gluconate	-	r
4-O-beta-D-glucopyranosyl-D-gluconate + phosphate	4-O-beta-D-glucopyranosyl-D-gluconate i.e. cellobionate. Phosphorolytic reaction follows a sequential bibi mechanism	Neurospora crassa	alpha-D-glucose 1-phosphate + D-gluconate	-	r
4-O-beta-D-glucopyranosyl-D-gluconate + phosphate	4-O-beta-D-glucopyranosyl-D-gluconate i.e. cellobionate. Phosphorolytic reaction follows a sequential bibi mechanism	Xanthomonas campestris	alpha-D-glucose 1-phosphate + D-gluconate	-	r
4-O-beta-D-glucopyranosyl-D-gluconate + phosphate	it is suggested that the enzyme plays a crucial role in cellulose degradation where cellobionic acid as oxidized cellulolytic product is converted into alpha-D-glucose 1-phosphate and D-gluconic acid to enter glycolysis and the pentose phosphate pathway, respectively	Neurospora crassa DSM 1257	alpha-D-glucose 1-phosphate + D-gluconate	-	r
4-O-beta-D-glucopyranosyl-D-gluconate + phosphate	4-O-beta-D-glucopyranosyl-D-gluconate i.e. cellobionate. Phosphorolytic reaction follows a sequential bibi mechanism	Neurospora crassa DSM 1257	alpha-D-glucose 1-phosphate + D-gluconate	-	r
4-O-beta-D-glucopyranosyl-D-gluconate + phosphate	-	Xanthomonas campestris pv. campestris ATCC 33913	alpha-D-glucose 1-phosphate + D-gluconate	-	r
alpha-D-glucose 1 phosphate + D-gluconate	-	Neurospora crassa	4-O-beta-D-glucopyranosyl-D-gluconate + phosphate	-	r
alpha-D-glucose 1 phosphate + D-gluconate	-	Neurospora crassa DSM 1257	4-O-beta-D-glucopyranosyl-D-gluconate + phosphate	-	r
alpha-D-glucose 1-phosphate + D-gluconate	-	Xanthomonas campestris pv. campestris	4-O-beta-D-glucopyranosyl-D-gluconate + phosphate	-	r
alpha-D-glucose 1-phosphate + D-gluconate	-	Neurospora crassa	4-O-beta-D-glucopyranosyl-D-gluconate + phosphate	-	r
alpha-D-glucose 1-phosphate + D-gluconate	-	Xanthomonas campestris	4-O-beta-D-glucopyranosyl-D-gluconate + phosphate	-	r
alpha-D-glucose 1-phosphate + D-gluconate	-	Neurospora crassa DSM 1257	4-O-beta-D-glucopyranosyl-D-gluconate + phosphate	-	r
alpha-D-glucose 1-phosphate + D-gluconate	-	Xanthomonas campestris pv. campestris ATCC 33913	4-O-beta-D-glucopyranosyl-D-gluconate + phosphate	-	r
alpha-D-glucose 1-phosphate + D-glucuronate	-	Xanthomonas campestris pv. campestris	4-O-beta-D-glucopyranosyl-D-glucuronate + phosphate	-	r
alpha-D-glucose 1-phosphate + D-glucuronate	in the synthetic direction the enzyme produces 4-O-beta-D-glucopyranosyl-D-glucuronate from alpha-D-glucose 1-phosphate and D-glucuronate with low activity	Neurospora crassa	4-O-beta-D-glucopyranosyl-D-glucuronate + phosphate	-	?
alpha-D-glucose 1-phosphate + D-glucuronate	in the synthetic direction the enzyme produces 4-O-beta-D-glucopyranosyl-D-glucuronate from alpha-D-glucose 1-phosphate and D-glucuronate with low activity	Xanthomonas campestris	4-O-beta-D-glucopyranosyl-D-glucuronate + phosphate	-	?
alpha-D-glucose 1-phosphate + D-glucuronate	-	Xanthomonas campestris pv. campestris ATCC 33913	4-O-beta-D-glucopyranosyl-D-glucuronate + phosphate	-	r

Subunits

Subunits	Comment	Organism
homodimer	2 * 84000, SDS-PAGE	Xanthomonas campestris
homodimer	2 * 87000, SDS-PAGE	Neurospora crassa
homodimer	2 * 84000, SDS-PAGE, 2 * 89700, amino acid sequence	Xanthomonas campestris pv. campestris
homodimer	2 * 87000, SDS-PAGE, 2 * 90796, amino acid sequence	Neurospora crassa
homodimer	2 * 89700, calculated from sequence	Xanthomonas campestris
homodimer	2 * 90769, calculated from sequence	Neurospora crassa

Synonyms

Synonyms	Comment	Organism
4-O-beta-D-glucopyranosyl-D-gluconic acid: phosphate alpha-D-glucosyltransferase	-	Neurospora crassa
4-O-beta-D-glucopyranosyl-D-gluconic acid: phosphate alpha-D-glucosyltransferase	-	Xanthomonas campestris
NCU09425	locus name	Neurospora crassa
XCC4077	locus name	Xanthomonas campestris

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	assay at	Neurospora crassa
30	-	assay at	Xanthomonas campestris

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
35	-	15 min, stable	Neurospora crassa
35	-	15 min, stable	Xanthomonas campestris
35	-	15 min stable up to	Xanthomonas campestris pv. campestris
35	-	15 min stable up to	Neurospora crassa

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism
43	-	D-glucuronate	pH 6.5, 30°C	Xanthomonas campestris
43	-	D-glucuronate	pH 6.0-6.5, 30°C	Xanthomonas campestris pv. campestris
127	-	alpha-D-glucose 1-phosphate	pH 6.5, 30°C	Xanthomonas campestris
127	-	alpha-D-glucose 1-phosphate	pH 6.0-6.5, 30°C	Xanthomonas campestris pv. campestris
146	-	D-gluconate	pH 6.5, 30°C	Neurospora crassa
146	-	D-gluconate	pH 6.0-6.5, 30°C	Neurospora crassa
173	-	D-gluconate	pH 6.5, 30°C	Xanthomonas campestris
173	-	D-gluconate	pH 6.0-6.5, 30°C	Xanthomonas campestris pv. campestris
306	-	phosphate	pH 7.0, 30°C	Neurospora crassa
306	-	4-O-beta-D-glucopyranosyl-D-gluconate	pH 7.0, 30°C	Neurospora crassa

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6	-	synthetic activity	Xanthomonas campestris
6	-	synthetic reaction	Xanthomonas campestris pv. campestris
6.5	-	synthetic activity	Neurospora crassa
6.5	-	synthetic reaction	Neurospora crassa
7	-	phosphorolytic reaction	Xanthomonas campestris pv. campestris
7	-	phosphorolytic reaction	Neurospora crassa
7	-	phosphorolytic activity	Neurospora crassa
7	-	phosphorolytic activity	Xanthomonas campestris

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
5	10	4°C, 24 h, stable	Xanthomonas campestris
5	10.5	-	Xanthomonas campestris pv. campestris
5.5	9	-	Neurospora crassa
5.5	9	4°C, 24 h, stable	Neurospora crassa

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
7.3	-	4-O-beta-D-glucopyranosyl-D-gluconate	pH 7.0, 30°C	Neurospora crassa

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism
18	-	D-glucuronate	pH 6.5, 30°C	Xanthomonas campestris
87	-	D-gluconate	pH 6.5, 30°C	Xanthomonas campestris
605	-	alpha-D-glucose 1-phosphate	pH 6.5, 30°C	Xanthomonas campestris