Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli DH5alpha | Bacteroides thetaiotaomicron |
expression in Escherichia coli | Bacteroides thetaiotaomicron |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.6 | - |
phosphate | pH 5.5, 30°C, cosubstrate: 4-O-beta-D-mannopyranosyl-N-acetyl-D-glucosamine, phosphorolytic reaction | Bacteroides thetaiotaomicron | |
2.1 | - |
4-O-beta-D-mannopyranosyl-N-acetyl-D-glucosamine | pH 5.5, 30°C, cosubstrate: phosphate, phosphorolytic reaction | Bacteroides thetaiotaomicron | |
2.6 | - |
alpha-D-mannose 1-phosphate | pH 5.5, 30°C | Bacteroides thetaiotaomicron | |
2.6 | - |
alpha-D-mannose 1-phosphate | pH 5.5, 30°C, cosubstrate: N-acetyl-D-glucosamine, synthetic reaction | Bacteroides thetaiotaomicron | |
2.7 | - |
4-O-beta-N-acetyl-D-glucosaminyl-N-acetyl-D-glucosamine | pH 5.5, 30°C | Bacteroides thetaiotaomicron | |
3.8 | - |
N-acetyl-D-glucosamine | pH 5.5, 30°C | Bacteroides thetaiotaomicron | |
3.8 | - |
N-acetyl-D-glucosamine | pH 5.5, 30°C, synthetic reaction | Bacteroides thetaiotaomicron | |
27 | - |
N,N'-diacetylchitobiose | pH 5.5, 30°C, synthetic reaction | Bacteroides thetaiotaomicron |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Bacteroides thetaiotaomicron | 5737 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
4 * 35000, SDS-PAGE | Bacteroides thetaiotaomicron |
35000 | - |
4 * 35000, SDS-PAGE, 4 * 37823, amino acid sequence | Bacteroides thetaiotaomicron |
37823 | - |
4 * 35000, SDS-PAGE, 4 * 37823, amino acid sequence | Bacteroides thetaiotaomicron |
37823 | - |
4 * 37823, calculated from sequence | Bacteroides thetaiotaomicron |
147000 | - |
gel filtration | Bacteroides thetaiotaomicron |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-O-beta-D-mannopyranosyl-N-acetyl-D-glucosamine + phosphate | Bacteroides thetaiotaomicron | the enzyme is involved in the degradation of host-derived N-glycans | N-acetyl-D-glucosamine + alpha-D-mannose 1-phosphate | - |
r | |
4-O-beta-D-mannopyranosyl-N-acetyl-D-glucosamine + phosphate | Bacteroides thetaiotaomicron VPI-5482 | the enzyme is involved in the degradation of host-derived N-glycans | N-acetyl-D-glucosamine + alpha-D-mannose 1-phosphate | - |
r | |
4-O-beta-D-mannosyl-N-acetyl-D-glucosamine + phosphate | Bacteroides thetaiotaomicron | - |
N-acetyl-D-glucosamine + alpha-D-mannose 1-phosphate | - |
r | |
4-O-beta-D-mannosyl-N-acetyl-D-glucosamine + phosphate | Bacteroides thetaiotaomicron VPI-5482 | - |
N-acetyl-D-glucosamine + alpha-D-mannose 1-phosphate | - |
r | |
N-acetyl-D-glucosamine + alpha-D-mannose 1-phosphate | Bacteroides thetaiotaomicron | - |
4-O-beta-D-mannosyl-N-acetyl-D-glucosamine + phosphate | - |
r | |
N-acetyl-D-glucosamine + alpha-D-mannose 1-phosphate | Bacteroides thetaiotaomicron VPI-5482 | - |
4-O-beta-D-mannosyl-N-acetyl-D-glucosamine + phosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacteroides thetaiotaomicron | Q8A8X4 | - |
- |
Bacteroides thetaiotaomicron | Q8A8Y4 | - |
- |
Bacteroides thetaiotaomicron VPI-5482 | Q8A8X4 | - |
- |
Bacteroides thetaiotaomicron VPI-5482 | Q8A8Y4 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Bacteroides thetaiotaomicron |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
4-O-beta-D-mannopyranosyl-N-acetyl-D-glucosamine + phosphate = N-acetyl-D-glucosamine + alpha-D-mannose 1-phosphate | sequential Bi Bi mechanism | Bacteroides thetaiotaomicron |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
30 | - |
pH 5.5, 30°C, substrate: N,N'-diacetylchitobiose | Bacteroides thetaiotaomicron |
258 | - |
pH 5.5, 30°C, substrate: N-acetyl-D-glucosamine | Bacteroides thetaiotaomicron |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-O-beta-D-mannopyranosyl-N-acetyl-D-glucosamine + phosphate | the enzyme is involved in the degradation of host-derived N-glycans | Bacteroides thetaiotaomicron | N-acetyl-D-glucosamine + alpha-D-mannose 1-phosphate | - |
r | |
4-O-beta-D-mannopyranosyl-N-acetyl-D-glucosamine + phosphate | the enzyme is involved in the degradation of host-derived N-glycans | Bacteroides thetaiotaomicron VPI-5482 | N-acetyl-D-glucosamine + alpha-D-mannose 1-phosphate | - |
r | |
4-O-beta-D-mannosyl-N-acetyl-D-glucosamine + phosphate | - |
Bacteroides thetaiotaomicron | N-acetyl-D-glucosamine + alpha-D-mannose 1-phosphate | - |
r | |
4-O-beta-D-mannosyl-N-acetyl-D-glucosamine + phosphate | - |
Bacteroides thetaiotaomicron VPI-5482 | N-acetyl-D-glucosamine + alpha-D-mannose 1-phosphate | - |
r | |
4-O-beta-N-acetyl-D-glucosaminyl-N-acetyl-D-glucosamine + alpha-D-mannose 1-phosphate | - |
Bacteroides thetaiotaomicron | beta-N-acetyl-D-glucosaminyl-(1->4)-beta-N-acetyl-D-glucosaminyl-(1->4)-D-mannose + phosphate | - |
r | |
4-O-beta-N-acetyl-D-glucosaminyl-N-acetyl-D-glucosamine + alpha-D-mannose 1-phosphate | - |
Bacteroides thetaiotaomicron VPI-5482 | beta-N-acetyl-D-glucosaminyl-(1->4)-beta-N-acetyl-D-glucosaminyl-(1->4)-D-mannose + phosphate | - |
r | |
beta-N-acetyl-D-glucosaminyl-(1->4)-beta-N-acetyl-D-glucosaminyl-(1->4)-D-mannose + phosphate | - |
Bacteroides thetaiotaomicron | 4-O-beta-N-acetyl-D-glucosaminyl-N-acetyl-D-glucosamine + alpha-D-mannose 1-phosphate | - |
r | |
beta-N-acetyl-D-glucosaminyl-(1->4)-beta-N-acetyl-D-glucosaminyl-(1->4)-D-mannose + phosphate | - |
Bacteroides thetaiotaomicron VPI-5482 | 4-O-beta-N-acetyl-D-glucosaminyl-N-acetyl-D-glucosamine + alpha-D-mannose 1-phosphate | - |
r | |
additional information | very low activity with D-glucose or D-mannose as acceptor and alpha-D-mannose 1-phosphate as donor | Bacteroides thetaiotaomicron | ? | - |
? | |
additional information | very low activity with D-glucose or D-mannose as acceptor and alpha-D-mannose 1-phosphate as donor | Bacteroides thetaiotaomicron VPI-5482 | ? | - |
? | |
N,N'-diacetylchitobiose + alpha-D-mannose 1-phosphate | - |
Bacteroides thetaiotaomicron | beta-1,4-D-mannopyranosyl-beta-1,4-N-acetyl-D-glucosaminyl-beta-1,4-N-acetyl-D-glucosamine + phosphate | - |
r | |
N,N'-diacetylchitobiose + alpha-D-mannose 1-phosphate | - |
Bacteroides thetaiotaomicron VPI-5482 | beta-1,4-D-mannopyranosyl-beta-1,4-N-acetyl-D-glucosaminyl-beta-1,4-N-acetyl-D-glucosamine + phosphate | - |
r | |
N-acetyl-D-glucosamine + alpha-D-mannose 1-phosphate | - |
Bacteroides thetaiotaomicron | 4-O-beta-D-mannosyl-N-acetyl-D-glucosamine + phosphate | - |
r | |
N-acetyl-D-glucosamine + alpha-D-mannose 1-phosphate | - |
Bacteroides thetaiotaomicron VPI-5482 | 4-O-beta-D-mannosyl-N-acetyl-D-glucosamine + phosphate | - |
r | |
N-acetyl-D-glucosamine + alpha-D-mannose 1-phosphate | - |
Bacteroides thetaiotaomicron | 4-O-beta-D-mannopyranosyl-N-acetyl-D-glucosamine + phosphate | - |
r | |
N-acetyl-D-glucosamine + alpha-D-mannose 1-phosphate | - |
Bacteroides thetaiotaomicron VPI-5482 | 4-O-beta-D-mannopyranosyl-N-acetyl-D-glucosamine + phosphate | - |
r |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 35000, SDS-PAGE | Bacteroides thetaiotaomicron |
homotetramer | 4 * 35000, SDS-PAGE, 4 * 37823, amino acid sequence | Bacteroides thetaiotaomicron |
homotetramer | 4 * 37823, calculated from sequence | Bacteroides thetaiotaomicron |
Synonyms | Comment | Organism |
---|---|---|
beta-1,4-D-mannosyl-N-acetyl-D-glucosamine phosphorylase | - |
Bacteroides thetaiotaomicron |
BT1033 | - |
Bacteroides thetaiotaomicron |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Bacteroides thetaiotaomicron |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
15 min, stable up to | Bacteroides thetaiotaomicron |
55 | - |
15 min stable up to | Bacteroides thetaiotaomicron |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
22 | - |
4-O-beta-N-acetyl-D-glucosaminyl-N-acetyl-D-glucosamine | pH 5.5, 30°C | Bacteroides thetaiotaomicron | |
22 | - |
N,N'-diacetylchitobiose | pH 5.5, 30°C, synthetic reaction | Bacteroides thetaiotaomicron | |
25 | - |
alpha-D-mannose 1-phosphate | pH 5.5, 30°C | Bacteroides thetaiotaomicron | |
25 | - |
alpha-D-mannose 1-phosphate | pH 5.5, 30°C, cosubstrate: N-acetyl-D-glucosamine, synthetic reaction | Bacteroides thetaiotaomicron | |
37 | - |
N-acetyl-D-glucosamine | pH 5.5, 30°C | Bacteroides thetaiotaomicron | |
37 | - |
N-acetyl-D-glucosamine | pH 5.5, 30°C, synthetic reaction | Bacteroides thetaiotaomicron | |
240 | - |
phosphate | pH 5.5, 30°C, cosubstrate: 4-O-beta-D-mannopyranosyl-N-acetyl-D-glucosamine, phosphorolytic reaction | Bacteroides thetaiotaomicron | |
240 | - |
4-O-beta-D-mannopyranosyl-N-acetyl-D-glucosamine | pH 5.5, 30°C, cosubstrate: phosphate, phosphorolytic reaction | Bacteroides thetaiotaomicron |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
- |
Bacteroides thetaiotaomicron |
5.5 | - |
phosphorolytic reaction | Bacteroides thetaiotaomicron |
5.5 | - |
synthetic reaction | Bacteroides thetaiotaomicron |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4.5 | 10.5 | 4°C, 24 h, stable | Bacteroides thetaiotaomicron |
4.5 | 10.5 | 4°C, 24 h stable | Bacteroides thetaiotaomicron |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme is involved in the degradation of host-derived N-glycans | Bacteroides thetaiotaomicron |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.8 | - |
N,N'-diacetylchitobiose | pH 5.5, 30°C, synthetic reaction | Bacteroides thetaiotaomicron | |
1.9 | - |
beta-1,4-D-mannopyranosyl-beta-1,4-N-acetyl-D-glucosaminyl-beta-1,4-N-acetyl-D-glucosamine | pH 5.5, 30°C, cosubstrate: phosphate, phosphorolytic reaction | Bacteroides thetaiotaomicron | |
9.6 | - |
alpha-D-mannose 1-phosphate | pH 5.5, 30°C, cosubstrate: N-acetyl-D-glucosamine, synthetic reaction | Bacteroides thetaiotaomicron | |
9.6 | - |
N-acetyl-D-glucosamine | pH 5.5, 30°C, synthetic reaction | Bacteroides thetaiotaomicron | |
110 | - |
4-O-beta-D-mannopyranosyl-N-acetyl-D-glucosamine | pH 5.5, 30°C, cosubstrate: phosphate, phosphorolytic reaction | Bacteroides thetaiotaomicron |