Cloned (Comment) | Organism |
---|---|
heterologously expressed as a C-terminal His6-tagged protein in Escherichia coli | Amycolatopsis orientalis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.107 | - |
desvancosaminyl-vancomycin | pH 7.5, 37°C | Amycolatopsis orientalis | |
0.218 | - |
dTDP-beta-L-4-epi-vancosamine | pH 7.5, 37°C | Amycolatopsis orientalis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dTDP-beta-L-4-epi-vancosamine + devancosaminyl-vancomycin | Amycolatopsis orientalis | the enzyme is involved in the biosynthesis of the glycopeptide antibiotic chloroeremomycin | dTDP + chloroorienticin B | - |
? | |
dTDP-beta-L-4-epi-vancosamine + devancosaminyl-vancomycin | Amycolatopsis orientalis A82846 | the enzyme is involved in the biosynthesis of the glycopeptide antibiotic chloroeremomycin | dTDP + chloroorienticin B | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Amycolatopsis orientalis | P96558 | - |
- |
Amycolatopsis orientalis A82846 | P96558 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Amycolatopsis orientalis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dTDP-beta-L-4-epi-vancosamine + aglucovancomycin | low activity | Amycolatopsis orientalis | dTDP + chloroorienticin C | - |
? | |
dTDP-beta-L-4-epi-vancosamine + aglucovancomycin | low activity | Amycolatopsis orientalis A82846 | dTDP + chloroorienticin C | - |
? | |
dTDP-beta-L-4-epi-vancosamine + desvancosaminyl-vancomycin | - |
Amycolatopsis orientalis | dTDP + chloroorienticin B | - |
? | |
dTDP-beta-L-4-epi-vancosamine + desvancosaminyl-vancomycin | - |
Amycolatopsis orientalis A82846 | dTDP + chloroorienticin B | - |
? | |
dTDP-beta-L-4-epi-vancosamine + devancosaminyl-vancomycin | the enzyme is involved in the biosynthesis of the glycopeptide antibiotic chloroeremomycin | Amycolatopsis orientalis | dTDP + chloroorienticin B | - |
? | |
dTDP-beta-L-4-epi-vancosamine + devancosaminyl-vancomycin | i.e. desvancosaminyl-vancomycin. Devancoaminyl vancomycin is the preferred acceptor for GtfA. GtfA is unable to use UDP-4-epi-vancosamine as the sugar substrate. GtfA catalyzes the addition of 4-epi-vancosamine to the hydroxyl group of beta-OH-Tyr-6 of devancoaminyl vancomycin. GtfA also transfers 4-epi-vancosamine to the 2-amino analogue of devancoaminyl vancomycin to produce the 2-amino analogue of chloroorienticin B | Amycolatopsis orientalis | dTDP + chloroorienticin B | i.e. 4-epi-balhimicin | ? | |
dTDP-beta-L-4-epi-vancosamine + devancosaminyl-vancomycin | the enzyme is involved in the biosynthesis of the glycopeptide antibiotic chloroeremomycin | Amycolatopsis orientalis A82846 | dTDP + chloroorienticin B | - |
? | |
dTDP-beta-L-4-epi-vancosamine + devancosaminyl-vancomycin | i.e. desvancosaminyl-vancomycin. Devancoaminyl vancomycin is the preferred acceptor for GtfA. GtfA is unable to use UDP-4-epi-vancosamine as the sugar substrate. GtfA catalyzes the addition of 4-epi-vancosamine to the hydroxyl group of beta-OH-Tyr-6 of devancoaminyl vancomycin. GtfA also transfers 4-epi-vancosamine to the 2-amino analogue of devancoaminyl vancomycin to produce the 2-amino analogue of chloroorienticin B | Amycolatopsis orientalis A82846 | dTDP + chloroorienticin B | i.e. 4-epi-balhimicin | ? | |
dTDP-beta-L-4-epi-vancosamine + epivancomycin | low activity | Amycolatopsis orientalis | dTDP + chloroeremomycin | - |
? | |
dTDP-beta-L-4-epi-vancosamine + epivancomycin | low activity | Amycolatopsis orientalis A82846 | dTDP + chloroeremomycin | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GtfA | - |
Amycolatopsis orientalis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Amycolatopsis orientalis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | GtfA transfers 4-epi-vancosamine to vancomycin aglycone or epivancomycin at much reduced rates of less than 0.0008/s | Amycolatopsis orientalis | |
0.038 | - |
desvancosaminyl-vancomycin | pH 7.5, 37°C | Amycolatopsis orientalis | |
0.038 | - |
dTDP-beta-L-4-epi-vancosamine | pH 7.5, 37°C | Amycolatopsis orientalis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Amycolatopsis orientalis |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme is involved in the biosynthesis of chloroeremomycin | Amycolatopsis orientalis |