Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, 1.8 resolution | Amycolatopsis orientalis |
Protein Variants | Comment | Organism |
---|---|---|
D13A | kcat/Km is 9.2% compared to the wild-type value | Amycolatopsis orientalis |
D332A | kcat/Km is 0.16% compared to the wild-type value | Amycolatopsis orientalis |
H125A | kcat/Km is 3.8fold higher than the wild-type value | Amycolatopsis orientalis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.45 | - |
UDP-alpha-D-glucose | mutant enzyme H125A, pH and temperature not specified in the publication | Amycolatopsis orientalis | |
1.1 | - |
UDP-alpha-D-glucose | mutant enzyme D13A, pH and temperature not specified in the publication | Amycolatopsis orientalis | |
1.3 | - |
UDP-alpha-D-glucose | wild-type enzyme, pH and temperature not specified in the publication | Amycolatopsis orientalis | |
3.3 | - |
UDP-alpha-D-glucose | mutant enzyme D332A, pH and temperature not specified in the publication | Amycolatopsis orientalis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-alpha-D-glucose + vancomycin aglycone | Amycolatopsis orientalis | the enzyme is involved in the biosynthesis of the glycopeptide antibiotic chloroeremomycin | UDP + devancoaminyl vancomycin | - |
? | |
UDP-alpha-D-glucose + vancomycin aglycone | Amycolatopsis orientalis A82846 | the enzyme is involved in the biosynthesis of the glycopeptide antibiotic chloroeremomycin | UDP + devancoaminyl vancomycin | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Amycolatopsis orientalis | P96559 | - |
- |
Amycolatopsis orientalis A82846 | P96559 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Amycolatopsis orientalis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-alpha-D-glucose + vancomycin aglycone | the enzyme is involved in the biosynthesis of the glycopeptide antibiotic chloroeremomycin | Amycolatopsis orientalis | UDP + devancoaminyl vancomycin | - |
? | |
UDP-alpha-D-glucose + vancomycin aglycone | the enzyme transfers the glucose residue from UDP-glucose to the 4-OH-PheGly4 residue of the vancomycin aglycone | Amycolatopsis orientalis | UDP + devancoaminyl vancomycin | - |
? | |
UDP-alpha-D-glucose + vancomycin aglycone | the enzyme is involved in the biosynthesis of the glycopeptide antibiotic chloroeremomycin | Amycolatopsis orientalis A82846 | UDP + devancoaminyl vancomycin | - |
? | |
UDP-alpha-D-glucose + vancomycin aglycone | the enzyme transfers the glucose residue from UDP-glucose to the 4-OH-PheGly4 residue of the vancomycin aglycone | Amycolatopsis orientalis A82846 | UDP + devancoaminyl vancomycin | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GtfB | - |
Amycolatopsis orientalis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0022 | - |
UDP-alpha-D-glucose | mutant enzyme D332A, pH and temperature not specified in the publication | Amycolatopsis orientalis | |
0.043 | - |
UDP-alpha-D-glucose | mutant enzyme D13A, pH and temperature not specified in the publication | Amycolatopsis orientalis | |
0.55 | - |
UDP-alpha-D-glucose | wild-type enzyme, pH and temperature not specified in the publication | Amycolatopsis orientalis | |
0.72 | - |
UDP-alpha-D-glucose | mutant enzyme H125A, pH and temperature not specified in the publication | Amycolatopsis orientalis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00067 | - |
UDP-alpha-D-glucose | mutant enzyme D332A, pH and temperature not specified in the publication | Amycolatopsis orientalis | |
0.039 | - |
UDP-alpha-D-glucose | mutant enzyme D13A, pH and temperature not specified in the publication | Amycolatopsis orientalis | |
0.42 | - |
UDP-alpha-D-glucose | wild-type enzyme, pH and temperature not specified in the publication | Amycolatopsis orientalis | |
1.6 | - |
UDP-alpha-D-glucose | mutant enzyme H125A, pH and temperature not specified in the publication | Amycolatopsis orientalis |