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Literature summary for 2.4.1.310 extracted from

  • Mulichak, A.M.; Losey, H.C.; Walsh, C.T.; Garavito, R.M.
    Structure of the UDP-glucosyltransferase GtfB that modifies the heptapeptide aglycone in the biosynthesis of vancomycin group antibiotics (2001), Structure, 9, 547-557.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, 1.8 resolution Amycolatopsis orientalis

Protein Variants

Protein Variants Comment Organism
D13A kcat/Km is 9.2% compared to the wild-type value Amycolatopsis orientalis
D332A kcat/Km is 0.16% compared to the wild-type value Amycolatopsis orientalis
H125A kcat/Km is 3.8fold higher than the wild-type value Amycolatopsis orientalis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.45
-
UDP-alpha-D-glucose mutant enzyme H125A, pH and temperature not specified in the publication Amycolatopsis orientalis
1.1
-
UDP-alpha-D-glucose mutant enzyme D13A, pH and temperature not specified in the publication Amycolatopsis orientalis
1.3
-
UDP-alpha-D-glucose wild-type enzyme, pH and temperature not specified in the publication Amycolatopsis orientalis
3.3
-
UDP-alpha-D-glucose mutant enzyme D332A, pH and temperature not specified in the publication Amycolatopsis orientalis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-alpha-D-glucose + vancomycin aglycone Amycolatopsis orientalis the enzyme is involved in the biosynthesis of the glycopeptide antibiotic chloroeremomycin UDP + devancoaminyl vancomycin
-
?
UDP-alpha-D-glucose + vancomycin aglycone Amycolatopsis orientalis A82846 the enzyme is involved in the biosynthesis of the glycopeptide antibiotic chloroeremomycin UDP + devancoaminyl vancomycin
-
?

Organism

Organism UniProt Comment Textmining
Amycolatopsis orientalis P96559
-
-
Amycolatopsis orientalis A82846 P96559
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Amycolatopsis orientalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-alpha-D-glucose + vancomycin aglycone the enzyme is involved in the biosynthesis of the glycopeptide antibiotic chloroeremomycin Amycolatopsis orientalis UDP + devancoaminyl vancomycin
-
?
UDP-alpha-D-glucose + vancomycin aglycone the enzyme transfers the glucose residue from UDP-glucose to the 4-OH-PheGly4 residue of the vancomycin aglycone Amycolatopsis orientalis UDP + devancoaminyl vancomycin
-
?
UDP-alpha-D-glucose + vancomycin aglycone the enzyme is involved in the biosynthesis of the glycopeptide antibiotic chloroeremomycin Amycolatopsis orientalis A82846 UDP + devancoaminyl vancomycin
-
?
UDP-alpha-D-glucose + vancomycin aglycone the enzyme transfers the glucose residue from UDP-glucose to the 4-OH-PheGly4 residue of the vancomycin aglycone Amycolatopsis orientalis A82846 UDP + devancoaminyl vancomycin
-
?

Synonyms

Synonyms Comment Organism
GtfB
-
Amycolatopsis orientalis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0022
-
UDP-alpha-D-glucose mutant enzyme D332A, pH and temperature not specified in the publication Amycolatopsis orientalis
0.043
-
UDP-alpha-D-glucose mutant enzyme D13A, pH and temperature not specified in the publication Amycolatopsis orientalis
0.55
-
UDP-alpha-D-glucose wild-type enzyme, pH and temperature not specified in the publication Amycolatopsis orientalis
0.72
-
UDP-alpha-D-glucose mutant enzyme H125A, pH and temperature not specified in the publication Amycolatopsis orientalis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.00067
-
UDP-alpha-D-glucose mutant enzyme D332A, pH and temperature not specified in the publication Amycolatopsis orientalis
0.039
-
UDP-alpha-D-glucose mutant enzyme D13A, pH and temperature not specified in the publication Amycolatopsis orientalis
0.42
-
UDP-alpha-D-glucose wild-type enzyme, pH and temperature not specified in the publication Amycolatopsis orientalis
1.6
-
UDP-alpha-D-glucose mutant enzyme H125A, pH and temperature not specified in the publication Amycolatopsis orientalis