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Literature summary for 2.4.1.31 extracted from

  • Abi, A.; Wang, A.; Joerdening, H.J.
    Continuous laminaribiose production using an immobilized bienzymatic system in a packed bed reactor (2018), Appl. Biochem. Biotechnol., 186, 861-876 .
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
D-glucose competitive inhibition Euglena gracilis
laminaribiose
-
Euglena gracilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.001
-
D-glucose at pH 6.0 and 35°C Euglena gracilis
0.00414
-
laminaribiose at pH 6.0 and 35°C Euglena gracilis
0.44
-
alpha-D-glucose 1-phosphate at pH 6.0 and 35°C Euglena gracilis

Organism

Organism UniProt Comment Textmining
Euglena gracilis
-
-
-
Euglena gracilis Z
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partially purified using ammonium sulfate precipitation Euglena gracilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-D-glucose 1-phosphate + D-glucose
-
Euglena gracilis laminaribiose + phosphate
-
?
alpha-D-glucose 1-phosphate + D-glucose
-
Euglena gracilis Z laminaribiose + phosphate
-
?
laminaribiose + alpha-D-glucose 1-phosphate
-
Euglena gracilis laminaritriose + phosphate
-
?
laminaribiose + alpha-D-glucose 1-phosphate
-
Euglena gracilis Z laminaritriose + phosphate
-
?

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
16.65
-
D-glucose at pH 6.0 and 35°C Euglena gracilis
6680
-
laminaribiose at pH 6.0 and 35°C Euglena gracilis