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Literature summary for 2.4.1.31 extracted from

  • Mueller, C.; Ortmann, T.; Abi, A.; Hartig, D.; Scholl, S.; Joerdening, H.J.
    Immobilization and characterization of E. gracilis extract with enriched laminaribiose phosphorylase activity for bienzymatic production of laminaribiose (2017), Appl. Biochem. Biotechnol., 182, 197-215 .
    View publication on PubMed

General Stability

General Stability Organism
immobilization on Sepabeads EC-EP/S results in 65% enzyme activity. The immobilized enzyme loses 48% of its activity when used 7 times. Laminaribiose phosphorylase that is encapsulated in chitosan or bentonite retains 75% activity Euglena gracilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
alpha-D-glucose 1-phosphate + D-glucose Euglena gracilis
-
laminaribiose + phosphate
-
?
laminaribiose + alpha-D-glucose 1-phosphate Euglena gracilis
-
laminaritriose + phosphate
-
?

Organism

Organism UniProt Comment Textmining
Euglena gracilis
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining

Storage Stability

Storage Stability Organism
20°C, 0.05 M sodium phosphate buffer (pH 6.3), 89 days, 84% loss of activity Euglena gracilis
4°C, 0.05 M sodium phosphate buffer (pH 6.3), 89 days, 48% loss of activity Euglena gracilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-D-glucose 1-phosphate + D-glucose
-
Euglena gracilis laminaribiose + phosphate
-
?
laminaribiose + alpha-D-glucose 1-phosphate
-
Euglena gracilis laminaritriose + phosphate
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
the half-life of the native enzyme at 37°C is 8.7 h. The half-life of the immobilized enzyme is 8.9 h Euglena gracilis