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Literature summary for 2.4.1.31 extracted from

  • Kitaoka, M.; Matsuoka, Y.; Mori, K.; Nishimoto, M.; Hayashi, K.
    Characterization of a bacterial laminaribiose phosphorylase (2012), Biosci. Biotechnol. Biochem., 76, 343-348.
    View publication on PubMed
Search for more literature for 2.4.1.31

Inhibitors

Inhibitors Comment Organism Structure
D-glucose higher concentrations of glucose cause competitive substrate inhibition Paenibacillus sp.
laminaribiose
-
 Paenibacillus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.14
-
 phosphate pH 7.0, 45°C Paenibacillus sp.
0.48
-
 laminaribiose pH 7.0, 45°C Paenibacillus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
100000
-
 SDS-PAGE, monomer Paenibacillus sp.
200000
-
 gel filtration, dimer Paenibacillus sp.

Organism

Organism UniProt Comment Textmining
Paenibacillus sp. D7UT17
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
laminaribiose + phosphate
-
 Paenibacillus sp. alpha-D-glucose 1-phosphate + D-glucose
-
 r
laminariheptaose + phosphate only weak phosphorylation Paenibacillus sp. ?
-
 r
laminarihexaose + phosphate only weak phosphorylation Paenibacillus sp. ?
-
 r
laminaripentaose + phosphate only weak phosphorylation Paenibacillus sp. ?
-
 r
laminaritetraose + phosphate only weak phosphorylation Paenibacillus sp. ?
-
 r
laminaritriose + phosphate only weak phosphorylation Paenibacillus sp. ?
-
 r
additional information on reverse phosphorolysis enzyme is most active on glucose as acceptor and is weakly active on several monosaccharides (e.g. mannose) with alpha-D-glucose 1-phosphate as donor Paenibacillus sp. ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 100000 Da Paenibacillus sp.

Synonyms

Synonyms Comment Organism
LBPbac
-
 Paenibacillus sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
 assay at Paenibacillus sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 over 30 min at pH 7 Paenibacillus sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
19
-
 laminaribiose co-substrate: phosphate, pH 7.0, 45°C Paenibacillus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
 6.8-7.0 optimal pH Paenibacillus sp.
7
-
 assay at Paenibacillus sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 9
-
 Paenibacillus sp.

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
9.8
-
 laminaribiose pH 7.0, 45°C Paenibacillus sp.