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Literature summary for 2.4.1.31 extracted from

  • Kitaoka, M.; Sasaki, T.; Taniguchi, H.
    Purification and properties of laminaribiose phosphorylase (EC 2.4 1.31) from Euglena gracilis Z (1993), Arch. Biochem. Biophys., 304, 508-514.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
D-glucose substrate inhibition at high concentration Euglena gracilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
120000
-
2 * 120000, SDS-PAGE Euglena gracilis
200000
-
gel filtration Euglena gracilis

Organism

Organism UniProt Comment Textmining
Euglena gracilis
-
var. bacillaris, IAM E-2 and IAM E-3, Z. IAM E-6
-

Purification (Commentary)

Purification (Comment) Organism
3 isoforms: F0, FI, FII Euglena gracilis

Reaction

Reaction Comment Organism Reaction ID
3-beta-D-glucosyl-D-glucose + phosphate = D-glucose + alpha-D-glucose 1-phosphate ordered bi-bi mechanism Euglena gracilis

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Euglena gracilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-beta-D-glucosyl-D-glucose + phosphate
-
Euglena gracilis D-glucose + alpha-D-glucose 1-phosphate
-
r
alpha-D-glucose 1-phosphate + 1,5-anhydro-D-glucitol
-
Euglena gracilis ?
-
r
alpha-D-glucose 1-phosphate + cellobiose
-
Euglena gracilis phosphate + oligosaccharides
-
r
alpha-D-glucose 1-phosphate + D-glucose initial rate of synthetic reaction is 4 times faster than that of phosphorolytic reaction Euglena gracilis laminaribiose + laminaritriose + laminaritetraose + laminaripentaose + phosphate
-
r
alpha-D-glucose 1-phosphate + laminaribiose
-
Euglena gracilis ?
-
r
alpha-D-glucose 1-phosphate + m-nitrophenyl-beta-D-glucoside
-
Euglena gracilis ?
-
r
alpha-D-glucose 1-phosphate + methyl beta-D-glucoside
-
Euglena gracilis ?
-
?
alpha-D-glucose 1-phosphate + p-nitrophenyl-beta-D-glucoside
-
Euglena gracilis ?
-
r
alpha-D-glucose 1-phosphate + phenyl beta-D-glucoside
-
Euglena gracilis ?
-
r
alpha-D-glucose 1-phosphate + salicin
-
Euglena gracilis ?
-
r
laminaripentaose + phosphate
-
Euglena gracilis laminaritetraose + alpha-D-glucose 1-phosphate
-
r
laminaritetraose + phosphate
-
Euglena gracilis laminaritriose + alpha-D-glucose 1-phosphate
-
r
laminaritriose + phosphate
-
Euglena gracilis laminaribiose + alpha-D-glucose 1-phosphate
-
r
additional information weak or no activity observed with other carbohydrates Euglena gracilis ?
-
?

Subunits

Subunits Comment Organism
dimer 2 * 120000, SDS-PAGE Euglena gracilis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Euglena gracilis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
35
-
isoenzyme F1, stable below Euglena gracilis
40
-
isoenzymes F0 and F2, stable below Euglena gracilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
in Tris-HCl buffer Euglena gracilis