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Literature summary for 2.4.1.31 extracted from
- Purification and properties of laminaribiose phosphorylase (EC 2.4 1.31) from Euglena gracilis Z (1993), Arch. Biochem. Biophys., 304, 508-514.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| D-glucose | substrate inhibition at high concentration | Euglena gracilis |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 120000 | - |
2 * 120000, SDS-PAGE | Euglena gracilis |
| 200000 | - |
gel filtration | Euglena gracilis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Euglena gracilis | - |
var. bacillaris, IAM E-2 and IAM E-3, Z. IAM E-6 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 3 isoforms: F0, FI, FII | Euglena gracilis |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| 3-beta-D-glucosyl-D-glucose + phosphate = D-glucose + alpha-D-glucose 1-phosphate | ordered bi-bi mechanism | Euglena gracilis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Euglena gracilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-beta-D-glucosyl-D-glucose + phosphate | - |
Euglena gracilis | D-glucose + alpha-D-glucose 1-phosphate | - |
r | |
| alpha-D-glucose 1-phosphate + 1,5-anhydro-D-glucitol | - |
Euglena gracilis | ? | - |
r | |
| alpha-D-glucose 1-phosphate + cellobiose | - |
Euglena gracilis | phosphate + oligosaccharides | - |
r | |
| alpha-D-glucose 1-phosphate + D-glucose | initial rate of synthetic reaction is 4 times faster than that of phosphorolytic reaction | Euglena gracilis | laminaribiose + laminaritriose + laminaritetraose + laminaripentaose + phosphate | - |
r | |
| alpha-D-glucose 1-phosphate + laminaribiose | - |
Euglena gracilis | ? | - |
r | |
| alpha-D-glucose 1-phosphate + m-nitrophenyl-beta-D-glucoside | - |
Euglena gracilis | ? | - |
r | |
| alpha-D-glucose 1-phosphate + methyl beta-D-glucoside | - |
Euglena gracilis | ? | - |
? | |
| alpha-D-glucose 1-phosphate + p-nitrophenyl-beta-D-glucoside | - |
Euglena gracilis | ? | - |
r | |
| alpha-D-glucose 1-phosphate + phenyl beta-D-glucoside | - |
Euglena gracilis | ? | - |
r | |
| alpha-D-glucose 1-phosphate + salicin | - |
Euglena gracilis | ? | - |
r | |
| laminaripentaose + phosphate | - |
Euglena gracilis | laminaritetraose + alpha-D-glucose 1-phosphate | - |
r | |
| laminaritetraose + phosphate | - |
Euglena gracilis | laminaritriose + alpha-D-glucose 1-phosphate | - |
r | |
| laminaritriose + phosphate | - |
Euglena gracilis | laminaribiose + alpha-D-glucose 1-phosphate | - |
r | |
| additional information | weak or no activity observed with other carbohydrates | Euglena gracilis | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 120000, SDS-PAGE | Euglena gracilis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Euglena gracilis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
isoenzyme F1, stable below | Euglena gracilis |
| 40 | - |
isoenzymes F0 and F2, stable below | Euglena gracilis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | - |
in Tris-HCl buffer | Euglena gracilis |
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