BRENDA - Enzyme Database
show all sequences of 2.4.1.31

beta-1,3-Oligoglucan: orthophosphate glucosyltransferase from Euglena gracilis

Goldemberg, S.H.; Marechal, L.R.; De Souza, B.C.; J. Biol. Chem. 241, 45-50 (1966)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
additional information
not activated by 1mM AMP, cyclic 3',5'-AMP, ADP, ATP, NAD, NADH, UDP-glucose, UDP-acetyl-glucosamine, UDP, phosphoryl-enolpyruvate, pyridoxal-phosphate, vitamin B12, glucose-6-phosphate, fructose 1,6-diphosphate, 3-phosphate-glyceric acid, 6-phosphogluconate, beta-glycero-phosphate, alpha- and beta-methylglucoside, sucrose, xylose, arabinose, galactose, sorbitol, mannitol, KNC, KF, barium acetate, ammonium sulfate
Euglena gracilis
General Stability
General Stability
Organism
stable to repeated freezing and thawing
Euglena gracilis
Inhibitors
Inhibitors
Commentary
Organism
Structure
2-deoxyglucose
25-30% inhibition at 20 mM
Euglena gracilis
D-fructose
25-30% inhibition at 20 mM
Euglena gracilis
D-glucosamine
25-30% inhibition at 20 mM
Euglena gracilis
D-glucose
25-30% inhibition at 10 mM
Euglena gracilis
D-mannose
25-30% inhibition at 20 mM
Euglena gracilis
diphosphate
10% inhibition at 1 mM, pH 7
Euglena gracilis
fructose 6-phosphate
10% inhibition at 1 mM
Euglena gracilis
HgCl2
10% inhibition at 1 mM
Euglena gracilis
imidazole
weak, 25% inhibition at 0.4 M
Euglena gracilis
additional information
not inhibited by 1 mM AMP, cyclic 3',5'-AMP, ADP, ATP, NAD+, NADH, UDP-glucose, UDP-acetyl-glucosamine, UDP, phosphoryl-enolpyruvate, pyridoxal-phosphate, vitamin B12, glucose-6-phosphate, fructose 1,6-diphosphate, glyceric acid 3-phosphate, 6-phosphogluconate, beta-glycero-phosphate, alpha- and beta-methylglucoside, sucrose, xylose, arabinose, galactose, sorbitol, mannitol, KNC, KF, barium acetate, ammonium sulfate
Euglena gracilis
NADP+
10% inhibition at 1 mM
Euglena gracilis
p-hydroxymercuribenzoate
40% inhibition at 0.2 mM and 80% inhibition at 2 mM
Euglena gracilis
phloridzin
10% inhibition at 1 mM
Euglena gracilis
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
linear within certain limits
Euglena gracilis
2.1
-
D-glucose 1-phosphate
with glucose addition
Euglena gracilis
2.5
-
phosphate
with laminaribiose addition
Euglena gracilis
5
-
laminaribiose
with phosphate addition
Euglena gracilis
6
-
laminaritriose
with phosphate addition
Euglena gracilis
19
-
D-glucose
with glucose 1-phosphate addition
Euglena gracilis
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Euglena gracilis
-
-
-
Purification (Commentary)
Commentary
Organism
protamine sulfate and ammonium sulfate precipitation, calcium phosphate gel adsorption and DEAE-cellulose chromatography
Euglena gracilis
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
24
-
D-glucose + D-glucose 1-phosphate, in DEAE-eluate
Euglena gracilis
Storage Stability
Storage Stability
Organism
-14C, 3 months stable
Euglena gracilis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3-beta-D-glucosyl-D-glucose + phosphate
-
489045
Euglena gracilis
D-glucose + alpha-D-glucose 1-phosphate
-
489045
Euglena gracilis
r
alpha-D-glucose 1-phosphate + 2-deoxy-D-glucose
-
489045
Euglena gracilis
?
-
-
-
r
alpha-D-glucose 1-phosphate + arbutin
-
489045
Euglena gracilis
?
-
-
-
r
alpha-D-glucose 1-phosphate + cellobiose
-
489045
Euglena gracilis
phosphate + oligosaccharides
-
-
-
r
alpha-D-glucose 1-phosphate + D-glucose
specific for alpha-D-glucose 1-phosphate as glucosyl donor
489045
Euglena gracilis
laminaribiose + laminaritriose + laminaritetraose + laminaripentaose
amount of individual products depends on substrate concentration and reaction time
489045
Euglena gracilis
-
alpha-D-glucose 1-phosphate + laminaribiose
-
489045
Euglena gracilis
?
-
-
-
r
alpha-D-glucose 1-phosphate + laminaribiosyl-beta-1,4-glucose
-
489045
Euglena gracilis
?
-
-
-
r
alpha-D-glucose 1-phosphate + laminaritetraose
-
489045
Euglena gracilis
?
-
-
-
r
alpha-D-glucose 1-phosphate + laminaritriose
-
489045
Euglena gracilis
?
-
-
-
r
alpha-D-glucose 1-phosphate + methyl beta-D-glucoside
-
489045
Euglena gracilis
?
-
-
-
?
alpha-D-glucose 1-phosphate + phenyl beta-D-glucoside
-
489045
Euglena gracilis
?
-
-
-
r
alpha-D-glucose 1-phosphate + salicin
-
489045
Euglena gracilis
?
-
-
-
r
laminaripentaose + phosphate
-
489045
Euglena gracilis
laminaritetraose + alpha-D-glucose 1-phosphate
-
489045
Euglena gracilis
r
laminaritetraose + phosphate
-
489045
Euglena gracilis
laminaritriose + alpha-D-glucose 1-phosphate
-
489045
Euglena gracilis
r
laminaritriose + phosphate
-
489045
Euglena gracilis
laminaribiose + alpha-D-glucose 1-phosphate
-
489045
Euglena gracilis
r
additional information
arsenolysis of laminaribiose is observed, but not that of glucose 1-phosphate
489045
Euglena gracilis
?
-
-
-
-
additional information
weak or no activity observed with other carbohydrates
489045
Euglena gracilis
?
-
-
-
-
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
37
-
assay at
Euglena gracilis
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.3
6.9
same optimum pH for for forward and reverse reaction
Euglena gracilis
6.5
-
assay at
Euglena gracilis
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
additional information
not activated by 1mM AMP, cyclic 3',5'-AMP, ADP, ATP, NAD, NADH, UDP-glucose, UDP-acetyl-glucosamine, UDP, phosphoryl-enolpyruvate, pyridoxal-phosphate, vitamin B12, glucose-6-phosphate, fructose 1,6-diphosphate, 3-phosphate-glyceric acid, 6-phosphogluconate, beta-glycero-phosphate, alpha- and beta-methylglucoside, sucrose, xylose, arabinose, galactose, sorbitol, mannitol, KNC, KF, barium acetate, ammonium sulfate
Euglena gracilis
General Stability (protein specific)
General Stability
Organism
stable to repeated freezing and thawing
Euglena gracilis
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
2-deoxyglucose
25-30% inhibition at 20 mM
Euglena gracilis
D-fructose
25-30% inhibition at 20 mM
Euglena gracilis
D-glucosamine
25-30% inhibition at 20 mM
Euglena gracilis
D-glucose
25-30% inhibition at 10 mM
Euglena gracilis
D-mannose
25-30% inhibition at 20 mM
Euglena gracilis
diphosphate
10% inhibition at 1 mM, pH 7
Euglena gracilis
fructose 6-phosphate
10% inhibition at 1 mM
Euglena gracilis
HgCl2
10% inhibition at 1 mM
Euglena gracilis
imidazole
weak, 25% inhibition at 0.4 M
Euglena gracilis
additional information
not inhibited by 1 mM AMP, cyclic 3',5'-AMP, ADP, ATP, NAD+, NADH, UDP-glucose, UDP-acetyl-glucosamine, UDP, phosphoryl-enolpyruvate, pyridoxal-phosphate, vitamin B12, glucose-6-phosphate, fructose 1,6-diphosphate, glyceric acid 3-phosphate, 6-phosphogluconate, beta-glycero-phosphate, alpha- and beta-methylglucoside, sucrose, xylose, arabinose, galactose, sorbitol, mannitol, KNC, KF, barium acetate, ammonium sulfate
Euglena gracilis
NADP+
10% inhibition at 1 mM
Euglena gracilis
p-hydroxymercuribenzoate
40% inhibition at 0.2 mM and 80% inhibition at 2 mM
Euglena gracilis
phloridzin
10% inhibition at 1 mM
Euglena gracilis
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
linear within certain limits
Euglena gracilis
2.1
-
D-glucose 1-phosphate
with glucose addition
Euglena gracilis
2.5
-
phosphate
with laminaribiose addition
Euglena gracilis
5
-
laminaribiose
with phosphate addition
Euglena gracilis
6
-
laminaritriose
with phosphate addition
Euglena gracilis
19
-
D-glucose
with glucose 1-phosphate addition
Euglena gracilis
Purification (Commentary) (protein specific)
Commentary
Organism
protamine sulfate and ammonium sulfate precipitation, calcium phosphate gel adsorption and DEAE-cellulose chromatography
Euglena gracilis
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
24
-
D-glucose + D-glucose 1-phosphate, in DEAE-eluate
Euglena gracilis
Storage Stability (protein specific)
Storage Stability
Organism
-14C, 3 months stable
Euglena gracilis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3-beta-D-glucosyl-D-glucose + phosphate
-
489045
Euglena gracilis
D-glucose + alpha-D-glucose 1-phosphate
-
489045
Euglena gracilis
r
alpha-D-glucose 1-phosphate + 2-deoxy-D-glucose
-
489045
Euglena gracilis
?
-
-
-
r
alpha-D-glucose 1-phosphate + arbutin
-
489045
Euglena gracilis
?
-
-
-
r
alpha-D-glucose 1-phosphate + cellobiose
-
489045
Euglena gracilis
phosphate + oligosaccharides
-
-
-
r
alpha-D-glucose 1-phosphate + D-glucose
specific for alpha-D-glucose 1-phosphate as glucosyl donor
489045
Euglena gracilis
laminaribiose + laminaritriose + laminaritetraose + laminaripentaose
amount of individual products depends on substrate concentration and reaction time
489045
Euglena gracilis
-
alpha-D-glucose 1-phosphate + laminaribiose
-
489045
Euglena gracilis
?
-
-
-
r
alpha-D-glucose 1-phosphate + laminaribiosyl-beta-1,4-glucose
-
489045
Euglena gracilis
?
-
-
-
r
alpha-D-glucose 1-phosphate + laminaritetraose
-
489045
Euglena gracilis
?
-
-
-
r
alpha-D-glucose 1-phosphate + laminaritriose
-
489045
Euglena gracilis
?
-
-
-
r
alpha-D-glucose 1-phosphate + methyl beta-D-glucoside
-
489045
Euglena gracilis
?
-
-
-
?
alpha-D-glucose 1-phosphate + phenyl beta-D-glucoside
-
489045
Euglena gracilis
?
-
-
-
r
alpha-D-glucose 1-phosphate + salicin
-
489045
Euglena gracilis
?
-
-
-
r
laminaripentaose + phosphate
-
489045
Euglena gracilis
laminaritetraose + alpha-D-glucose 1-phosphate
-
489045
Euglena gracilis
r
laminaritetraose + phosphate
-
489045
Euglena gracilis
laminaritriose + alpha-D-glucose 1-phosphate
-
489045
Euglena gracilis
r
laminaritriose + phosphate
-
489045
Euglena gracilis
laminaribiose + alpha-D-glucose 1-phosphate
-
489045
Euglena gracilis
r
additional information
arsenolysis of laminaribiose is observed, but not that of glucose 1-phosphate
489045
Euglena gracilis
?
-
-
-
-
additional information
weak or no activity observed with other carbohydrates
489045
Euglena gracilis
?
-
-
-
-
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
37
-
assay at
Euglena gracilis
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.3
6.9
same optimum pH for for forward and reverse reaction
Euglena gracilis
6.5
-
assay at
Euglena gracilis
Other publictions for EC 2.4.1.31
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
719125
Kitaoka
Characterization of a bacteria ...
Paenibacillus sp., Paenibacillus sp. YM-1
Biosci. Biotechnol. Biochem.
76
343-348
2012
-
-
-
-
-
-
2
2
-
-
2
-
-
4
-
-
-
-
-
-
-
-
12
1
1
-
1
1
2
-
1
-
1
-
-
-
-
-
-
-
-
-
-
2
1
2
-
-
2
-
-
-
-
-
-
-
-
-
12
1
1
-
1
1
2
-
1
-
-
-
-
-
-
-
719230
Nihira
Characterization of a laminari ...
Acholeplasma laidlawii
Carbohydr. Res.
361C
49-54
2012
-
-
1
-
-
-
1
4
-
-
-
-
-
3
-
-
-
-
-
-
-
-
9
-
1
-
1
3
2
-
1
-
1
-
-
-
-
1
-
-
-
-
-
1
1
4
-
-
-
-
-
-
-
-
-
-
-
-
9
-
1
-
1
3
2
-
1
-
-
-
-
-
6
6
489042
Kitaoka
-
Carbohydrate-processing phosph ...
Euglena gracilis
Trends Glycosci. Glycotechnol.
14
35-50
2002
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
489046
Kitaoka
Purification and properties of ...
Euglena gracilis
Arch. Biochem. Biophys.
304
508-514
1993
-
-
-
-
-
-
1
-
-
-
2
-
-
4
-
-
1
1
-
1
1
-
14
1
1
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
1
-
1
1
-
14
1
1
-
2
-
1
-
-
-
-
-
-
-
-
-
489040
Marechal
beta-1,3-Oligoglucan: orthopho ...
Euglena gracilis
Biochim. Biophys. Acta
146
431-442
1967
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
489043
Manners
Studies on carbohydrate metabo ...
Astasia ocellata
Arch. Biochem. Biophys.
121
443-451
1967
1
-
-
-
-
1
-
-
-
1
-
-
-
2
-
-
-
-
-
-
-
-
10
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
10
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
489045
Goldemberg
beta-1,3-Oligoglucan: orthopho ...
Euglena gracilis
J. Biol. Chem.
241
45-50
1966
1
-
-
-
-
1
13
6
-
-
-
-
-
1
-
-
1
-
-
-
1
1
17
-
1
-
-
-
2
-
-
-
-
-
-
1
-
-
-
-
-
1
-
13
-
6
-
-
-
-
-
-
-
1
-
-
1
1
17
-
1
-
-
-
2
-
-
-
-
-
-
-
-
-