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Literature summary for 2.4.1.31 extracted from

  • Goldemberg, S.H.; Marechal, L.R.; De Souza, B.C.
    beta-1,3-Oligoglucan: orthophosphate glucosyltransferase from Euglena gracilis (1966), J. Biol. Chem., 241, 45-50.
    View publication on PubMed
Search for more literature for 2.4.1.31

Activating Compound

Activating Compound Comment Organism Structure
additional information not activated by 1mM AMP, cyclic 3',5'-AMP, ADP, ATP, NAD, NADH, UDP-glucose, UDP-acetyl-glucosamine, UDP, phosphoryl-enolpyruvate, pyridoxal-phosphate, vitamin B12, glucose-6-phosphate, fructose 1,6-diphosphate, 3-phosphate-glyceric acid, 6-phosphogluconate, beta-glycero-phosphate, alpha- and beta-methylglucoside, sucrose, xylose, arabinose, galactose, sorbitol, mannitol, KNC, KF, barium acetate, ammonium sulfate Euglena gracilis

General Stability

General Stability Organism
stable to repeated freezing and thawing Euglena gracilis

Inhibitors

Inhibitors Comment Organism Structure
2-deoxyglucose 25-30% inhibition at 20 mM Euglena gracilis
D-fructose 25-30% inhibition at 20 mM Euglena gracilis
D-glucosamine 25-30% inhibition at 20 mM Euglena gracilis
D-glucose 25-30% inhibition at 10 mM Euglena gracilis
D-mannose 25-30% inhibition at 20 mM Euglena gracilis
diphosphate 10% inhibition at 1 mM, pH 7 Euglena gracilis
fructose 6-phosphate 10% inhibition at 1 mM Euglena gracilis
HgCl2 10% inhibition at 1 mM Euglena gracilis
imidazole weak, 25% inhibition at 0.4 M Euglena gracilis
additional information not inhibited by 1 mM AMP, cyclic 3',5'-AMP, ADP, ATP, NAD+, NADH, UDP-glucose, UDP-acetyl-glucosamine, UDP, phosphoryl-enolpyruvate, pyridoxal-phosphate, vitamin B12, glucose-6-phosphate, fructose 1,6-diphosphate, glyceric acid 3-phosphate, 6-phosphogluconate, beta-glycero-phosphate, alpha- and beta-methylglucoside, sucrose, xylose, arabinose, galactose, sorbitol, mannitol, KNC, KF, barium acetate, ammonium sulfate Euglena gracilis
NADP+ 10% inhibition at 1 mM Euglena gracilis
p-hydroxymercuribenzoate 40% inhibition at 0.2 mM and 80% inhibition at 2 mM Euglena gracilis
phloridzin 10% inhibition at 1 mM Euglena gracilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information linear within certain limits Euglena gracilis
2.1
-
 alpha-D-glucose 1-phosphate with glucose addition Euglena gracilis
2.5
-
 phosphate with laminaribiose addition Euglena gracilis
5
-
 laminaribiose with phosphate addition Euglena gracilis
6
-
 laminaritriose with phosphate addition Euglena gracilis
19
-
 D-glucose with glucose 1-phosphate addition Euglena gracilis

Organism

Organism UniProt Comment Textmining
Euglena gracilis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
protamine sulfate and ammonium sulfate precipitation, calcium phosphate gel adsorption and DEAE-cellulose chromatography Euglena gracilis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
24
-
 D-glucose + D-glucose 1-phosphate, in DEAE-eluate Euglena gracilis

Storage Stability

Storage Stability Organism
-14°C, 3 months stable Euglena gracilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-beta-D-glucosyl-D-glucose + phosphate
-
 Euglena gracilis D-glucose + alpha-D-glucose 1-phosphate
-
 r
alpha-D-glucose 1-phosphate + 2-deoxy-D-glucose
-
 Euglena gracilis ?
-
 r
alpha-D-glucose 1-phosphate + arbutin
-
 Euglena gracilis ?
-
 r
alpha-D-glucose 1-phosphate + cellobiose
-
 Euglena gracilis phosphate + oligosaccharides
-
 r
alpha-D-glucose 1-phosphate + D-glucose specific for alpha-D-glucose 1-phosphate as glucosyl donor Euglena gracilis laminaribiose + laminaritriose + laminaritetraose + laminaripentaose + phosphate amount of individual products depends on substrate concentration and reaction time ?
alpha-D-glucose 1-phosphate + laminaribiose
-
 Euglena gracilis ?
-
 r
alpha-D-glucose 1-phosphate + laminaribiosyl-beta-1,4-glucose
-
 Euglena gracilis ?
-
 r
alpha-D-glucose 1-phosphate + laminaritetraose
-
 Euglena gracilis ?
-
 r
alpha-D-glucose 1-phosphate + laminaritriose
-
 Euglena gracilis ?
-
 r
alpha-D-glucose 1-phosphate + methyl beta-D-glucoside
-
 Euglena gracilis ?
-
 ?
alpha-D-glucose 1-phosphate + phenyl beta-D-glucoside
-
 Euglena gracilis ?
-
 r
alpha-D-glucose 1-phosphate + salicin
-
 Euglena gracilis ?
-
 r
D-glucose + alpha-D-glucose 1-phosphate
-
 Euglena gracilis 3-beta-D-glucosyl-D-glucose + phosphate
-
 ?
laminaripentaose + phosphate
-
 Euglena gracilis laminaritetraose + alpha-D-glucose 1-phosphate
-
 r
laminaritetraose + phosphate
-
 Euglena gracilis laminaritriose + alpha-D-glucose 1-phosphate
-
 r
laminaritriose + phosphate
-
 Euglena gracilis laminaribiose + alpha-D-glucose 1-phosphate
-
 r
additional information arsenolysis of laminaribiose is observed, but not that of glucose 1-phosphate Euglena gracilis ?
-
 ?
additional information weak or no activity observed with other carbohydrates Euglena gracilis ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Euglena gracilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3 6.9 same optimum pH for for forward and reverse reaction Euglena gracilis
6.5
-
 assay at Euglena gracilis