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Literature summary for 2.4.1.31 extracted from

  • Manners, D.J.; Taylor, D.C.
    Studies on carbohydrate metabolizing enzymes. XVI. Specificity of laminaribiose phosphorylase from Astasia ocellata (1967), Arch. Biochem. Biophys., 121, 443-451.
    View publication on PubMed
Search for more literature for 2.4.1.31

Activating Compound

Activating Compound Comment Organism Structure
EDTA markedly increased activity Astasia ocellata

General Stability

General Stability Organism
aqueous extracts not stable Astasia ocellata

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ slight activation Astasia ocellata

Organism

Organism UniProt Comment Textmining
Astasia ocellata
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-beta-D-glucosyl-D-glucose + phosphate the enzyme is specific for the alpha-isomer Astasia ocellata D-glucose + alpha-D-glucose 1-phosphate
-
 r
alpha-D-glucose 1-phosphate + cellobiose
-
 Astasia ocellata phosphate + oligosaccharides oligosaccharides with two or more 1,3-linked glucose residues attached to the nonreducing residue of cellobiose r
alpha-D-glucose 1-phosphate + D-glucose at equilibrium synthesis of laminaribiose is favoured Astasia ocellata laminaribiose + laminaritriose + laminaritetraose + laminaripentaose + phosphate
-
 r
alpha-D-glucose 1-phosphate + D-glucose specific for alpha-D-glucose 1-phosphate as glucosyl donor Astasia ocellata laminaribiose + laminaritriose + laminaritetraose + laminaripentaose + phosphate
-
 r
alpha-D-glucose 1-phosphate + laminaribiose
-
 Astasia ocellata ?
-
 r
alpha-D-glucose 1-phosphate + laminaritriose
-
 Astasia ocellata ?
-
 r
alpha-D-glucose 1-phosphate + methyl beta-D-glucoside
-
 Astasia ocellata ?
-
 ?
alpha-D-glucose 1-phosphate + phenyl beta-D-glucoside
-
 Astasia ocellata ?
-
 r
additional information in presence of arsenate, laminaribiose is completely converted into glucose Astasia ocellata ?
-
 ?
additional information weak or no activity observed with other carbohydrates Astasia ocellata ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Astasia ocellata

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
21
-
 5%, 9%, 24% and 37% loss of activity after 2 h, 6 h, 24 h and 48 h, respectively Astasia ocellata