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Literature summary for 2.4.1.301 extracted from

  • Park, J.; Park, S.; Nepal, K.; Han, A.; Ban, Y.; Yoo, Y.; Kim, E.; Kim, E.; Kim, D.; Sohng, J.; Yoon, Y.
    Discovery of parallel pathways of kanamycin biosynthesis allows antibiotic manipulation (2011), Nat. Chem. Biol., 7, 843-852.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Streptomyces venezuelae. Reconstructed the kanamycin pathway stepwise in a mutant strain of Streptomyces venezuelae lacking the genes for biosynthesis of the native deoxysugar by transplanting plasmids harboring different combinations of putative genes from Streptomyces kanamyceticus in Streptomyces venezuelae and then carrying out biochemical analysis and chemical characterization Streptomyces kanamyceticus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03
-
UDP-alpha-D-kanosamine 37°C, pH not specified in the publication, cosubstrate: paromamine Streptomyces kanamyceticus
0.05
-
paromamine 37°C, pH not specified in the publication, cosubstrate: UDP-alpha-D-kanosamine Streptomyces kanamyceticus
0.25
-
paromamine 37°C, pH not specified in the publication, cosubstrate: UDP-alpha-D-glucose Streptomyces kanamyceticus
0.26
-
UDP-alpha-D-glucose 37°C, pH not specified in the publication, cosubstrate: paromamine Streptomyces kanamyceticus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-alpha-D-kanosamine + 2'-deamino-2'-hydroxyneamine Streptomyces kanamyceticus the enzyme is involved in the biosynthetic pathway of kanamycins UDP + kanamycin A
-
?
UDP-alpha-D-kanosamine + 2'-deamino-2'-hydroxyparomamine Streptomyces kanamyceticus the enzyme is involved in the biosynthetic pathway of kanamycins UDP + kanamycin X
-
?
UDP-alpha-D-kanosamine + neamine Streptomyces kanamyceticus the enzyme is involved in the biosynthetic pathway of kanamycins UDP + kanamycin B
-
?
UDP-alpha-D-kanosamine + paromamine Streptomyces kanamyceticus the enzyme is involved in the biosynthetic pathway of kanamycins UDP + kanamycin C
-
?

Organism

Organism UniProt Comment Textmining
Streptomyces kanamyceticus Q65CC7
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme can also accept UDP-alpha-D-glucose with lower efficiency Streptomyces kanamyceticus ?
-
?
UDP-alpha-D-glucose + paromamine paromamine i.e. (1R,2R,3S,4R,6S)-4,6-diamino-2,3-dihydroxycyclohexyl 2-amino-2-deoxy-alpha-D-glucopyranoside. UDP-alpha-D-kanosamine is a better glycosyl donor than UDP-alpha-D-glucose Streptomyces kanamyceticus UDP + ?
-
?
UDP-alpha-D-kanosamine + 2'-deamino-2'-hydroxyneamine the enzyme is involved in the biosynthetic pathway of kanamycins Streptomyces kanamyceticus UDP + kanamycin A
-
?
UDP-alpha-D-kanosamine + 2'-deamino-2'-hydroxyneamine UDP-alpha-D-kanosamine i.e. uridine 5'-[3-(3-amino-3-deoxy-alpha-D-glucopyranosyl) diphosphate] Streptomyces kanamyceticus UDP + kanamycin A kanamycin A i.e. (1S,2R,3R,4S,6R)-4,6-diamino-3-(6-amino-6-deoxy-alpha-D-glucopyranosyloxy)-2-hydroxycyclohexyl 3-amino-3-deoxy-alpha-D-glucopyranoside ?
UDP-alpha-D-kanosamine + 2'-deamino-2'-hydroxyparomamine
-
Streptomyces kanamyceticus UDP + kanamycin X kanamycin X i.e. (1S,2R,3R,4S,6R)-4,6-diamino-3-(alpha-D-glucopyranosyloxy)-2-hydroxycyclohexyl 3-amino-3-deoxy-alpha-D-glucopyranoside ?
UDP-alpha-D-kanosamine + 2'-deamino-2'-hydroxyparomamine the enzyme is involved in the biosynthetic pathway of kanamycins Streptomyces kanamyceticus UDP + kanamycin X
-
?
UDP-alpha-D-kanosamine + neamine the enzyme is involved in the biosynthetic pathway of kanamycins Streptomyces kanamyceticus UDP + kanamycin B
-
?
UDP-alpha-D-kanosamine + neamine neamine i.e. (1R,2R,3S,4R,6S)-4,6-diamino-2,3-dihydroxycyclohexyl 2,6-diamino-2,6-dideoxy-alpha-D-glucopyranoside Streptomyces kanamyceticus UDP + kanamycin B kanamycin B i.e. (1R,2S,3S,4R,6S)-4,6-diamino-3-(3-amino-3-deoxy-alpha-D-glucopyranosyloxy)-2-hydroxycyclohexyl 2,6-diamino-2,6-dideoxy-alpha-D-glucopyranoside ?
UDP-alpha-D-kanosamine + paromamine the enzyme is involved in the biosynthetic pathway of kanamycins Streptomyces kanamyceticus UDP + kanamycin C
-
?
UDP-alpha-D-kanosamine + paromamine paromamine i.e. (1R,2R,3S,4R,6S)-4,6-diamino-2,3-dihydroxycyclohexyl 2-amino-2-deoxy-alpha-D-glucopyranoside Streptomyces kanamyceticus UDP + kanamycin C kanamycin C i.e. (1R,2S,3S,4R,6S)-4,6-diamino-3-(3-amino-3-deoxy-alpha-D-glucopyranosyloxy)-2-hydroxycyclohexyl 2-amino-2-deoxy-alpha-D-glucopyranoside ?

Synonyms

Synonyms Comment Organism
KanE
-
Streptomyces kanamyceticus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.053
-
UDP-alpha-D-glucose 37°C, pH not specified in the publication Streptomyces kanamyceticus
0.056
-
UDP-alpha-D-kanosamine 37°C, pH not specified in the publication Streptomyces kanamyceticus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.2
-
UDP-alpha-D-glucose 37°C, pH not specified in the publication, cosubstrate: paromamine Streptomyces kanamyceticus
1.87
-
UDP-alpha-D-kanosamine 37°C, pH not specified in the publication, cosubstrate: paromamine Streptomyces kanamyceticus