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Literature summary for 2.4.1.298 extracted from

  • He, F.; Chen, W.K.; Yu, K.J.; Ji, X.N.; Duan, C.Q.; Reeves, M.J.; Wang, J.
    Molecular and biochemical characterization of the UDP-glucose:anthocyanin 5-O-glucosyltransferase from Vitis amurensis (2015), Phytochemistry, 117, 363-372.
    View publication on PubMed
Search for more literature for 2.4.1.298

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 10 mM, complete inhibition Vitis amurensis
additional information 10 mM Fe2+, Mg2+ or Ca2+ have only slight effect on the reaction Vitis amurensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0809
-
 malvidin 3-O-beta-D-glucoside pH 7.0, 25°C Vitis amurensis
0.213
-
 UDP-alpha-D-glucose pH 7.0, 25°C Vitis amurensis

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Vitis amurensis 5737
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54000
-
 x * 54000, calculated, x * 70000, SDS-PAGE of recombinant tagged protein Vitis amurensis
70000
-
 x * 54000, calculated, x * 70000, SDS-PAGE of recombinant tagged protein Vitis amurensis

Organism

Organism UniProt Comment Textmining
Vitis amurensis W8QG94
-
-

Source Tissue

Source Tissue Comment Organism Textmining
berry
-
 Vitis amurensis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-alpha-D-glucose + cyanidin 3-O-beta-D-glucoside 67% of the activity with malvidin 3-O-beta-D-glucoside Vitis amurensis UDP + cyanidin 3,5-di-O-beta-D-glucoside
-
 ?
UDP-alpha-D-glucose + cyanidin 3-O-beta-D-rutinoside 64% of the activity with malvidin 3-O-beta-D-glucoside Vitis amurensis UDP + cyanidin 3-O-beta-D-rutinoside 5-O-beta-D-glucoside
-
 ?
UDP-alpha-D-glucose + delphinidin 3-O-beta-D-glucoside 62% of the activity with malvidin 3-O-beta-D-glucoside Vitis amurensis UDP + delphinidin 3,5-di-O-beta-D-glucoside
-
 ?
UDP-alpha-D-glucose + malvidin 3-O-beta-D-galactoside 128% of the activity with malvidin 3-O-beta-D-glucoside Vitis amurensis UDP + malvidin 3-O-beta-D-glucoside 5-O-beta-D-galactoside
-
 ?
UDP-alpha-D-glucose + malvidin 3-O-beta-D-glucoside malvidin 3-O-beta-D-glucoside, best glycosidic acceptor with donor UDP-alpha-D-glucose Vitis amurensis UDP + malvidin 3,5-di-O-beta-D-glucoside
-
 ?
UDP-alpha-D-glucose + pelargonidin 3-O-beta-D-glucoside 83% of the activity with malvidin 3-O-beta-D-glucoside Vitis amurensis UDP + pelargonidin 3,5-di-O-beta-D-glucoside
-
 ?
UDP-alpha-D-glucose + peonidin 3-O-beta-D-glucoside 77% of the activity with malvidin 3-O-beta-D-glucoside Vitis amurensis UDP + peonidin 3,5-di-O-beta-D-glucoside
-
 ?
UDP-alpha-D-glucose + petunidin 3-O-beta-D-glucoside 87% of the activity with malvidin 3-O-beta-D-glucoside Vitis amurensis UDP + petunidin 3,5-di-O-beta-D-glucoside
-
 ?

Subunits

Subunits Comment Organism
? x * 54000, calculated, x * 70000, SDS-PAGE of recombinant tagged protein Vitis amurensis

Synonyms

Synonyms Comment Organism
5GT
-
 Vitis amurensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
-
 Vitis amurensis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.279
-
 malvidin 3-O-beta-D-glucoside pH 7.0, 25°C Vitis amurensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Vitis amurensis

General Information

General Information Comment Organism
physiological function 5GT is a key enzyme in the biosynthesis of bis-glucosidic anthocyanins in Vitis amurensis grape berries Vitis amurensis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.45
-
 malvidin 3-O-beta-D-glucoside pH 7.0, 25°C Vitis amurensis