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Literature summary for 2.4.1.298 extracted from

  • Nakatsuka, T.; Sato, K.; Takahashi, H.; Yamamura, S.; Nishihara, M.
    Cloning and characterization of the UDP-glucose:anthocyanin 5-O-glucosyltransferase gene from blue-flowered gentian (2008), J. Exp. Bot., 59, 1241-1252.
    View publication on PubMed
Search for more literature for 2.4.1.298

Cloned(Commentary)

Cloned (Comment) Organism
gene Gt5GT7, DNA and amino acid sequence determination and analysis, phylogenetic tree, expression in Escherichia coli, functional expression in Nicotiana tabacum plants leading to accumulation of cyanidin 3-rutinoside-5-glucoside Gentiana triflora

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0209
-
 cyanidin 3-O-beta-D-glucoside pH 8.5, 30°C Gentiana triflora
0.0295
-
 delphinidin 3-O-beta-D-glucoside pH 8.5, 30°C Gentiana triflora

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-alpha-D-glucose + cyanidin 3-O-beta-D-glucoside Gentiana triflora
-
 UDP + cyanidin 3,5-di-O-beta-D-glucoside
-
 ?
UDP-alpha-D-glucose + cyanidin 3-O-beta-D-glucoside Gentiana triflora Maciry
-
 UDP + cyanidin 3,5-di-O-beta-D-glucoside
-
 ?
UDP-alpha-D-glucose + delphinidin 3-O-beta-D-glucoside Gentiana triflora best substrate UDP + delphinidin 3,5-di-O-beta-D-glucoside
-
 ?
UDP-alpha-D-glucose + delphinidin 3-O-beta-D-glucoside Gentiana triflora Maciry best substrate UDP + delphinidin 3,5-di-O-beta-D-glucoside
-
 ?

Organism

Organism UniProt Comment Textmining
Gentiana triflora B2NID7 gene Gt5GT7
-
Gentiana triflora Maciry B2NID7 gene Gt5GT7
-

Purification (Commentary)

Purification (Comment) Organism
recombinant Gt5GT7 from Escherichia coli Gentiana triflora

Source Tissue

Source Tissue Comment Organism Textmining
filament weak expression Gentiana triflora
-
additional information no expression in leaves, stems, and other floral organs Gentiana triflora
-
petal increased levels of Gt5GT7 transcripts in petals coincides with flower development Gentiana triflora
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information Gt5GT7 transfers a glucosyl moiety to anthocyanidin 3-glycosides but not to other flavonoid compounds. Delphinidin 3-glucoside, the precursor of gentiodelphin, is the best substrate among several anthocyanidin 3-glycosides. No activity with UDP-galactose as a UDP-sugar donor, and with delphinidin, cyanidin, pelargonidin, malvidin, kaempferol, apigenin, and naringenin as acceptor substrates Gentiana triflora ?
-
 ?
additional information Gt5GT7 transfers a glucosyl moiety to anthocyanidin 3-glycosides but not to other flavonoid compounds. Delphinidin 3-glucoside, the precursor of gentiodelphin, is the best substrate among several anthocyanidin 3-glycosides. No activity with UDP-galactose as a UDP-sugar donor, and with delphinidin, cyanidin, pelargonidin, malvidin, kaempferol, apigenin, and naringenin as acceptor substrates Gentiana triflora Maciry ?
-
 ?
UDP-alpha-D-glucose + cyanidin 3-O-beta-D-galactoside
-
 Gentiana triflora UDP + cyanidin 3-O-galactoside 5-O-beta-D-glucoside
-
 ?
UDP-alpha-D-glucose + cyanidin 3-O-beta-D-glucoside
-
 Gentiana triflora UDP + cyanidin 3,5-di-O-beta-D-glucoside
-
 ?
UDP-alpha-D-glucose + cyanidin 3-O-beta-D-glucoside 66% of the activity with delphinidin 3-O-glucoside Gentiana triflora UDP + cyanidin 3,5-di-O-beta-D-glucoside
-
 ?
UDP-alpha-D-glucose + cyanidin 3-O-beta-D-glucoside
-
 Gentiana triflora Maciry UDP + cyanidin 3,5-di-O-beta-D-glucoside
-
 ?
UDP-alpha-D-glucose + cyanidin 3-O-beta-D-glucoside 66% of the activity with delphinidin 3-O-glucoside Gentiana triflora Maciry UDP + cyanidin 3,5-di-O-beta-D-glucoside
-
 ?
UDP-alpha-D-glucose + cyanidin 3-O-beta-D-rutinoside
-
 Gentiana triflora UDP + cyanidin 3-O-beta-D-rutinoside 5-O-beta-D-glucoside
-
 ?
UDP-alpha-D-glucose + cyanidin 3-O-beta-D-rutinoside
-
 Gentiana triflora Maciry UDP + cyanidin 3-O-beta-D-rutinoside 5-O-beta-D-glucoside
-
 ?
UDP-alpha-D-glucose + delphinidin 3-O-beta-D-glucoside best substrate Gentiana triflora UDP + delphinidin 3,5-di-O-beta-D-glucoside
-
 ?
UDP-alpha-D-glucose + delphinidin 3-O-beta-D-glucoside best substrate Gentiana triflora Maciry UDP + delphinidin 3,5-di-O-beta-D-glucoside
-
 ?
UDP-alpha-D-glucose + malvidin 3-O-beta-D-glucoside 35% of the activity with delphinidin 3-O-glucoside Gentiana triflora UDP + malvidin 3,5-di-O-beta-D-glucoside
-
 ?
UDP-alpha-D-glucose + pelargonidin 3-O-beta-D-glucoside 45% of the activity with delphinidin 3-O-glucoside Gentiana triflora UDP + pelargonidin 3,5-di-O-beta-D-glucoside
-
 ?

Synonyms

Synonyms Comment Organism
5GT
-
 Gentiana triflora
Gt5GT7
-
 Gentiana triflora

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Gentiana triflora

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 9
-
 Gentiana triflora

General Information

General Information Comment Organism
evolution Gt5GT7 belongs to the 5GT subfamily of the glycosyltransferase superfamily of enzymes. The Gt5GT7 contains a 44 amino acid consensus signature sequence, the so-called plant secondary product glycosyltransferase (PSPG) motif, which is highly conserved among plant glycosyltransferases Gentiana triflora