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Literature summary for 2.4.1.298 extracted from

  • Janvary, L.; Hoffmann, T.; Pfeiffer, J.; Hausmann, L.; Töpfer, R.; Fischer, T.; Schwab, W.
    A double mutation in the anthocyanin 5-O-glucosyltransferase gene disrupts enzymatic activity in Vitis vinifera L. (2009), J. Agric. Food Chem., 57, 3512-3518.
    View publication on PubMed
Search for more literature for 2.4.1.298

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis of the enzyme from cultivar Regent, revealing five amino acid substitutions and a truncation at the C-terminus causing the inactivation of the enzyme Vitis vinifera

Protein Variants

Protein Variants Comment Organism
A259S naturally occuring mutation Vitis vinifera
I461M naturally occuring mutation Vitis vinifera
L110V naturally occuring mutation Vitis vinifera
L372 naturally occuring mutation Vitis vinifera
V121L for the naturally inactive enzyme from cultivar Regent, restoration of the C-terminus in the European allele alone proves to be insufficient for a reversal to a functional allele. An additional V121L transition located in close spatial vicinity of the catalytically active histidine in the active site of the nonfunctional protein is also essential to recover 5GT activity Vitis vinifera
V307L naturally occuring mutation Vitis vinifera

Organism

Organism UniProt Comment Textmining
Vitis vinifera
-
-
-
Vitis vinifera Regent
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-alpha-D-glucose + cyanidin 3-O-beta-D-glucoside
-
 Vitis vinifera UDP + cyanidin 3,5-di-O-beta-D-glucoside
-
 ?
UDP-alpha-D-glucose + cyanidin 3-O-beta-D-glucoside
-
 Vitis vinifera Regent UDP + cyanidin 3,5-di-O-beta-D-glucoside
-
 ?
UDP-alpha-D-glucose + cyanidin 3-O-beta-D-rutinoside
-
 Vitis vinifera UDP + cyanidin 3-O-beta-D-rutinoside 5-O-beta-D-glucoside
-
 ?
UDP-alpha-D-glucose + cyanidin 3-O-beta-D-rutinoside
-
 Vitis vinifera Regent UDP + cyanidin 3-O-beta-D-rutinoside 5-O-beta-D-glucoside
-
 ?
UDP-alpha-D-glucose + malvidin 3-O-beta-D-glucoside
-
 Vitis vinifera UDP + malvidin 3,5-di-O-beta-D-glucoside
-
 ?
UDP-alpha-D-glucose + malvidin 3-O-beta-D-glucoside
-
 Vitis vinifera Regent UDP + malvidin 3,5-di-O-beta-D-glucoside
-
 ?

Subunits

Subunits Comment Organism
More three-dimensional structure and modeling, overview Vitis vinifera

Synonyms

Synonyms Comment Organism
5GT
-
 Vitis vinifera
anthocyanin 5-O-glucosyltransferase
-
 Vitis vinifera

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Vitis vinifera

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Vitis vinifera

General Information

General Information Comment Organism
malfunction sequence analysis of the enzyme from cultivar Regent reveals five amino acid substitutions and a truncation at the C-terminus causing the inactivation of the enzyme Vitis vinifera