Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.4.1.298 extracted from

  • Ogata, J.; Sakamoto, T.; Yamaguchi, M.; Kawanobu, S.; Yoshitama, K.
    Isolation and characterization of anthocyanin 5-O-glucosyltransferase from flowers of Dahlia variabilis (2001), J. Plant Physiol., 158, 709-714.
No PubMed abstract available
Search for more literature for 2.4.1.298

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol
-
 Dahlia pinnata
dithioerythritol
-
 Dahlia pinnata

Inhibitors

Inhibitors Comment Organism Structure
Al3+
-
 Dahlia pinnata
Cu2+
-
 Dahlia pinnata
Fe3+
-
 Dahlia pinnata
Hg2+
-
 Dahlia pinnata
iodoacetic acid slight inhibition Dahlia pinnata
Mn2+
-
 Dahlia pinnata
additional information poor inhibition by EDTA and ascorbic acid Dahlia pinnata
NEM
-
 Dahlia pinnata
PCMB
-
 Dahlia pinnata
Zn2+
-
 Dahlia pinnata

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ slight activation Dahlia pinnata
Mg2+ slight activation Dahlia pinnata

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53000
-
 gel filtration Dahlia pinnata

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-alpha-D-glucose + cyanidin 3-O-(6-O-malonyl)-beta-D-glucoside Dahlia pinnata
-
 UDP + cyanidin 3-O-beta-D-glucosyl-5-O-(6-O-malonyl)-beta-D-glucoside
-
 ?
UDP-alpha-D-glucose + cyanidin 3-O-(6-O-malonyl)-beta-D-glucoside Dahlia pinnata Sou-Une
-
 UDP + cyanidin 3-O-beta-D-glucosyl-5-O-(6-O-malonyl)-beta-D-glucoside
-
 ?
UDP-alpha-D-glucose + cyanidin 3-O-beta-D-glucoside Dahlia pinnata
-
 UDP + cyanidin 3,5-di-O-beta-D-glucoside
-
 ?
UDP-alpha-D-glucose + cyanidin 3-O-beta-D-glucoside Dahlia pinnata Sou-Une
-
 UDP + cyanidin 3,5-di-O-beta-D-glucoside
-
 ?
UDP-alpha-D-glucose + pelargonidin 3-O-(6-O-malonyl)-beta-D-glucoside Dahlia pinnata
-
 UDP + pelargonidin 3-O-(6-O-malonyl)-beta-D-glucosyl-5-O-beta-D-glucoside
-
 ?
UDP-alpha-D-glucose + pelargonidin 3-O-beta-D-glucoside Dahlia pinnata
-
 UDP + pelargonidin 3,5-di-O-beta-D-glucoside
-
 ?
UDP-alpha-D-glucose + pelargonidin 3-O-beta-D-glucoside Dahlia pinnata Sou-Une
-
 UDP + pelargonidin 3,5-di-O-beta-D-glucoside
-
 ?

Organism

Organism UniProt Comment Textmining
Dahlia pinnata
-
-
-
Dahlia pinnata Sou-Une
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 88fold by ammonium sulfate fractionation, anion exchange chromatography, gel filtration, and another different step of anion exchange chromatography Dahlia pinnata

Source Tissue

Source Tissue Comment Organism Textmining
flower
-
 Dahlia pinnata
-
flower bud
-
 Dahlia pinnata
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.18
-
 purified enzyme, pH 8.0, 35°C Dahlia pinnata

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information neither ADP-glucose nor UDP-galactose could serve as glycosyl donor Dahlia pinnata ?
-
 ?
additional information neither ADP-glucose nor UDP-galactose could serve as glycosyl donor Dahlia pinnata Sou-Une ?
-
 ?
UDP-alpha-D-glucose + cyanidin 3-O-(6-O-malonyl)-beta-D-glucoside
-
 Dahlia pinnata UDP + cyanidin 3-O-beta-D-glucosyl-5-O-(6-O-malonyl)-beta-D-glucoside
-
 ?
UDP-alpha-D-glucose + cyanidin 3-O-(6-O-malonyl)-beta-D-glucoside
-
 Dahlia pinnata Sou-Une UDP + cyanidin 3-O-beta-D-glucosyl-5-O-(6-O-malonyl)-beta-D-glucoside
-
 ?
UDP-alpha-D-glucose + cyanidin 3-O-beta-D-glucoside
-
 Dahlia pinnata UDP + cyanidin 3,5-di-O-beta-D-glucoside
-
 ?
UDP-alpha-D-glucose + cyanidin 3-O-beta-D-glucoside
-
 Dahlia pinnata Sou-Une UDP + cyanidin 3,5-di-O-beta-D-glucoside
-
 ?
UDP-alpha-D-glucose + delphinidin 3-O-beta-D-glucoside low activity Dahlia pinnata UDP + delphinidin 3,5-di-O-beta-D-glucoside
-
 ?
UDP-alpha-D-glucose + delphinidin 3-O-beta-D-glucoside low activity Dahlia pinnata Sou-Une UDP + delphinidin 3,5-di-O-beta-D-glucoside
-
 ?
UDP-alpha-D-glucose + pelargonidin 3-O-(6-O-malonyl)-beta-D-glucoside
-
 Dahlia pinnata UDP + pelargonidin 3-O-(6-O-malonyl)-beta-D-glucosyl-5-O-beta-D-glucoside
-
 ?
UDP-alpha-D-glucose + pelargonidin 3-O-beta-D-glucoside
-
 Dahlia pinnata UDP + pelargonidin 3,5-di-O-beta-D-glucoside
-
 ?
UDP-alpha-D-glucose + pelargonidin 3-O-beta-D-glucoside
-
 Dahlia pinnata Sou-Une UDP + pelargonidin 3,5-di-O-beta-D-glucoside
-
 ?

Synonyms

Synonyms Comment Organism
5GT
-
 Dahlia pinnata
anthocyanin 5-O-glucosyltransferase
-
 Dahlia pinnata
UDP-glucose:anthocyanin 5-O-glucosyltransferase
-
 Dahlia pinnata

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
 assay at Dahlia pinnata

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Dahlia pinnata

pH Range

pH Minimum pH Maximum Comment Organism
6.5 8.7 50% of maximal activity at pH 6.5 and pH 8.7 Dahlia pinnata

pI Value

Organism Comment pI Value Maximum pI Value
Dahlia pinnata
-
-
 4.2