BRENDA - Enzyme Database
show all sequences of 2.4.1.298

Isolation and characterization of anthocyanin 5-O-glucosyltransferase from flowers of Dahlia variabilis

Ogata, J.; Sakamoto, T.; Yamaguchi, M.; Kawanobu, S.; Yoshitama, K.; J. Plant Physiol. 158, 709-714 (2001)
No PubMed abstract available

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
2-mercaptoethanol
-
Dahlia pinnata
dithioerythritol
-
Dahlia pinnata
Inhibitors
Inhibitors
Commentary
Organism
Structure
Al3+
-
Dahlia pinnata
Cu2+
-
Dahlia pinnata
Fe3+
-
Dahlia pinnata
Hg2+
-
Dahlia pinnata
iodoacetic acid
slight inhibition
Dahlia pinnata
Mn2+
-
Dahlia pinnata
additional information
poor inhibition by EDTA and ascorbic acid
Dahlia pinnata
NEM
-
Dahlia pinnata
PCMB
-
Dahlia pinnata
Zn2+
-
Dahlia pinnata
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Ca2+
slight activation
Dahlia pinnata
Mg2+
slight activation
Dahlia pinnata
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
53000
-
gel filtration
Dahlia pinnata
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
UDP-alpha-D-glucose + cyanidin 3-O-(6-O-malonyl)-beta-D-glucoside
Dahlia pinnata
-
UDP + cyanidin 3-O-beta-D-glucosyl-5-O-(6-O-malonyl)-beta-D-glucoside
-
-
?
UDP-alpha-D-glucose + cyanidin 3-O-(6-O-malonyl)-beta-D-glucoside
Dahlia pinnata Sou-Une
-
UDP + cyanidin 3-O-beta-D-glucosyl-5-O-(6-O-malonyl)-beta-D-glucoside
-
-
?
UDP-alpha-D-glucose + cyanidin 3-O-beta-D-glucoside
Dahlia pinnata
-
UDP + cyanidin 3,5-di-O-beta-D-glucoside
-
-
?
UDP-alpha-D-glucose + cyanidin 3-O-beta-D-glucoside
Dahlia pinnata Sou-Une
-
UDP + cyanidin 3,5-di-O-beta-D-glucoside
-
-
?
UDP-alpha-D-glucose + pelargonidin 3-O-(6-O-malonyl)-beta-D-glucoside
Dahlia pinnata
-
UDP + pelargonidin 3-O-(6-O-malonyl)-beta-D-glucosyl-5-O-beta-D-glucoside
-
-
?
UDP-alpha-D-glucose + pelargonidin 3-O-beta-D-glucoside
Dahlia pinnata
-
UDP + pelargonidin 3,5-di-O-beta-D-glucoside
-
-
?
UDP-alpha-D-glucose + pelargonidin 3-O-beta-D-glucoside
Dahlia pinnata Sou-Une
-
UDP + pelargonidin 3,5-di-O-beta-D-glucoside
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Dahlia pinnata
-
-
-
Dahlia pinnata Sou-Une
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
native enzyme 88fold by ammonium sulfate fractionation, anion exchange chromatography, gel filtration, and another different step of anion exchange chromatography
Dahlia pinnata
Source Tissue
Source Tissue
Commentary
Organism
Textmining
flower
-
Dahlia pinnata
-
flower bud
-
Dahlia pinnata
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.18
-
purified enzyme, pH 8.0, 35°C
Dahlia pinnata
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
additional information
neither ADP-glucose nor UDP-galactose could serve as glycosyl donor
720319
Dahlia pinnata
?
-
-
-
?
additional information
neither ADP-glucose nor UDP-galactose could serve as glycosyl donor
720319
Dahlia pinnata Sou-Une
?
-
-
-
?
UDP-alpha-D-glucose + cyanidin 3-O-(6-O-malonyl)-beta-D-glucoside
-
720319
Dahlia pinnata
UDP + cyanidin 3-O-beta-D-glucosyl-5-O-(6-O-malonyl)-beta-D-glucoside
-
-
-
?
UDP-alpha-D-glucose + cyanidin 3-O-(6-O-malonyl)-beta-D-glucoside
-
720319
Dahlia pinnata Sou-Une
UDP + cyanidin 3-O-beta-D-glucosyl-5-O-(6-O-malonyl)-beta-D-glucoside
-
-
-
?
UDP-alpha-D-glucose + cyanidin 3-O-beta-D-glucoside
-
720319
Dahlia pinnata
UDP + cyanidin 3,5-di-O-beta-D-glucoside
-
-
-
?
UDP-alpha-D-glucose + cyanidin 3-O-beta-D-glucoside
-
720319
Dahlia pinnata Sou-Une
UDP + cyanidin 3,5-di-O-beta-D-glucoside
-
-
-
?
UDP-alpha-D-glucose + delphinidin 3-O-beta-D-glucoside
low activity
720319
Dahlia pinnata
UDP + delphinidin 3,5-di-O-beta-D-glucoside
-
-
-
?
UDP-alpha-D-glucose + delphinidin 3-O-beta-D-glucoside
low activity
720319
Dahlia pinnata Sou-Une
UDP + delphinidin 3,5-di-O-beta-D-glucoside
-
-
-
?
UDP-alpha-D-glucose + pelargonidin 3-O-(6-O-malonyl)-beta-D-glucoside
-
720319
Dahlia pinnata
UDP + pelargonidin 3-O-(6-O-malonyl)-beta-D-glucosyl-5-O-beta-D-glucoside
-
-
-
?
UDP-alpha-D-glucose + pelargonidin 3-O-beta-D-glucoside
-
720319
Dahlia pinnata
UDP + pelargonidin 3,5-di-O-beta-D-glucoside
-
-
-
?
UDP-alpha-D-glucose + pelargonidin 3-O-beta-D-glucoside
-
720319
Dahlia pinnata Sou-Une
UDP + pelargonidin 3,5-di-O-beta-D-glucoside
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
5GT
-
Dahlia pinnata
anthocyanin 5-O-glucosyltransferase
-
Dahlia pinnata
UDP-glucose:anthocyanin 5-O-glucosyltransferase
-
Dahlia pinnata
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
35
-
assay at
Dahlia pinnata
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Dahlia pinnata
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6.5
8.7
50% of maximal activity at pH 6.5 and pH 8.7
Dahlia pinnata
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Dahlia pinnata
-
-
4.2
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
2-mercaptoethanol
-
Dahlia pinnata
dithioerythritol
-
Dahlia pinnata
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Al3+
-
Dahlia pinnata
Cu2+
-
Dahlia pinnata
Fe3+
-
Dahlia pinnata
Hg2+
-
Dahlia pinnata
iodoacetic acid
slight inhibition
Dahlia pinnata
Mn2+
-
Dahlia pinnata
additional information
poor inhibition by EDTA and ascorbic acid
Dahlia pinnata
NEM
-
Dahlia pinnata
PCMB
-
Dahlia pinnata
Zn2+
-
Dahlia pinnata
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Ca2+
slight activation
Dahlia pinnata
Mg2+
slight activation
Dahlia pinnata
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
53000
-
gel filtration
Dahlia pinnata
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
UDP-alpha-D-glucose + cyanidin 3-O-(6-O-malonyl)-beta-D-glucoside
Dahlia pinnata
-
UDP + cyanidin 3-O-beta-D-glucosyl-5-O-(6-O-malonyl)-beta-D-glucoside
-
-
?
UDP-alpha-D-glucose + cyanidin 3-O-(6-O-malonyl)-beta-D-glucoside
Dahlia pinnata Sou-Une
-
UDP + cyanidin 3-O-beta-D-glucosyl-5-O-(6-O-malonyl)-beta-D-glucoside
-
-
?
UDP-alpha-D-glucose + cyanidin 3-O-beta-D-glucoside
Dahlia pinnata
-
UDP + cyanidin 3,5-di-O-beta-D-glucoside
-
-
?
UDP-alpha-D-glucose + cyanidin 3-O-beta-D-glucoside
Dahlia pinnata Sou-Une
-
UDP + cyanidin 3,5-di-O-beta-D-glucoside
-
-
?
UDP-alpha-D-glucose + pelargonidin 3-O-(6-O-malonyl)-beta-D-glucoside
Dahlia pinnata
-
UDP + pelargonidin 3-O-(6-O-malonyl)-beta-D-glucosyl-5-O-beta-D-glucoside
-
-
?
UDP-alpha-D-glucose + pelargonidin 3-O-beta-D-glucoside
Dahlia pinnata
-
UDP + pelargonidin 3,5-di-O-beta-D-glucoside
-
-
?
UDP-alpha-D-glucose + pelargonidin 3-O-beta-D-glucoside
Dahlia pinnata Sou-Une
-
UDP + pelargonidin 3,5-di-O-beta-D-glucoside
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
native enzyme 88fold by ammonium sulfate fractionation, anion exchange chromatography, gel filtration, and another different step of anion exchange chromatography
Dahlia pinnata
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
flower
-
Dahlia pinnata
-
flower bud
-
Dahlia pinnata
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.18
-
purified enzyme, pH 8.0, 35°C
Dahlia pinnata
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
additional information
neither ADP-glucose nor UDP-galactose could serve as glycosyl donor
720319
Dahlia pinnata
?
-
-
-
?
additional information
neither ADP-glucose nor UDP-galactose could serve as glycosyl donor
720319
Dahlia pinnata Sou-Une
?
-
-
-
?
UDP-alpha-D-glucose + cyanidin 3-O-(6-O-malonyl)-beta-D-glucoside
-
720319
Dahlia pinnata
UDP + cyanidin 3-O-beta-D-glucosyl-5-O-(6-O-malonyl)-beta-D-glucoside
-
-
-
?
UDP-alpha-D-glucose + cyanidin 3-O-(6-O-malonyl)-beta-D-glucoside
-
720319
Dahlia pinnata Sou-Une
UDP + cyanidin 3-O-beta-D-glucosyl-5-O-(6-O-malonyl)-beta-D-glucoside
-
-
-
?
UDP-alpha-D-glucose + cyanidin 3-O-beta-D-glucoside
-
720319
Dahlia pinnata
UDP + cyanidin 3,5-di-O-beta-D-glucoside
-
-
-
?
UDP-alpha-D-glucose + cyanidin 3-O-beta-D-glucoside
-
720319
Dahlia pinnata Sou-Une
UDP + cyanidin 3,5-di-O-beta-D-glucoside
-
-
-
?
UDP-alpha-D-glucose + delphinidin 3-O-beta-D-glucoside
low activity
720319
Dahlia pinnata
UDP + delphinidin 3,5-di-O-beta-D-glucoside
-
-
-
?
UDP-alpha-D-glucose + delphinidin 3-O-beta-D-glucoside
low activity
720319
Dahlia pinnata Sou-Une
UDP + delphinidin 3,5-di-O-beta-D-glucoside
-
-
-
?
UDP-alpha-D-glucose + pelargonidin 3-O-(6-O-malonyl)-beta-D-glucoside
-
720319
Dahlia pinnata
UDP + pelargonidin 3-O-(6-O-malonyl)-beta-D-glucosyl-5-O-beta-D-glucoside
-
-
-
?
UDP-alpha-D-glucose + pelargonidin 3-O-beta-D-glucoside
-
720319
Dahlia pinnata
UDP + pelargonidin 3,5-di-O-beta-D-glucoside
-
-
-
?
UDP-alpha-D-glucose + pelargonidin 3-O-beta-D-glucoside
-
720319
Dahlia pinnata Sou-Une
UDP + pelargonidin 3,5-di-O-beta-D-glucoside
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
35
-
assay at
Dahlia pinnata
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Dahlia pinnata
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6.5
8.7
50% of maximal activity at pH 6.5 and pH 8.7
Dahlia pinnata
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Dahlia pinnata
-
-
4.2
Other publictions for EC 2.4.1.298
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
737289
Oka
-
Biosynthesis of galactofuranos ...
Vitis vinifera, Vitis vinifera Cabernet Sauvignon
Trends Glycosci. Glycotechnol.
28
E39-E45
2016
-
-
1
-
-
-
-
7
1
-
1
-
-
3
-
-
-
-
-
1
-
-
13
1
3
1
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
7
1
-
1
-
-
-
-
-
-
1
-
-
13
1
1
-
1
-
1
-
-
-
-
-
-
-
-
-
736957
He
Molecular and biochemical char ...
Vitis amurensis
Phytochemistry
117
363-372
2015
-
-
-
-
-
-
2
2
1
-
2
-
-
1
-
-
-
-
-
1
-
-
8
1
1
1
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
2
1
-
2
-
-
-
-
-
-
1
-
-
8
1
1
-
-
1
1
-
-
-
-
1
1
-
1
1
737275
Yang
Multiple loss-of-function 5-O- ...
Vitis vinifera
Theor. Appl. Genet.
127
2433-2451
2014
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
722430
Janvary
A double mutation in the antho ...
Vitis vinifera, Vitis vinifera Regent
J. Agric. Food Chem.
57
3512-3518
2009
-
-
1
-
6
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
6
1
2
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6
1
1
-
-
-
1
-
-
-
-
1
1
-
-
-
722872
Nakatsuka
Cloning and characterization o ...
Gentiana triflora, Gentiana triflora Maciry
J. Exp. Bot.
59
1241-1252
2008
-
-
1
-
-
-
-
2
-
-
-
4
-
10
-
-
1
-
-
3
-
-
13
-
2
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
2
-
-
-
4
-
-
-
1
-
3
-
-
13
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
720319
Ogata
-
Isolation and characterization ...
Dahlia pinnata, Dahlia pinnata Sou-Une
J. Plant Physiol.
158
709-714
2001
2
-
-
-
-
-
10
-
-
2
1
7
-
2
-
-
1
-
-
2
1
-
11
-
3
1
-
-
-
1
1
-
-
-
1
-
2
-
-
-
-
-
-
-
10
-
-
-
2
1
7
-
-
-
1
-
2
1
-
11
-
1
-
-
-
1
1
-
1
-
-
-
-
-
-
722615
Yamazaki
Molecular cloning and biochemi ...
Glandularia x hybrida, Perilla frutescens, Perilla frutescens Chirimenjiso Shikun
J. Biol. Chem.
274
7405-7411
1999
1
-
3
-
-
-
3
2
-
-
3
6
-
5
-
-
-
-
-
3
-
-
16
3
6
3
-
-
-
3
-
-
-
-
-
-
1
-
3
-
-
-
-
-
3
-
2
-
-
3
6
-
-
-
-
-
3
-
-
16
3
3
-
-
-
3
-
-
-
-
-
-
-
-
-