Protein Variants | Comment | Organism |
---|---|---|
H371R | site-directed mutagenesis, strain FA 1090, the mutation is sufficient to restore PglH activity in the wild-type FA1090 strain | Neisseria gonorrhoeae |
additional information | replacement of the strain N400 locus carrying the deleted form of ORFs 2 and 3 with those derived from the Neisseria gonorrhoeae strain FA1090 and from diverse neisserial strains into Neisseria gonorrhoeae strain N400 in which the pgl gene function and associated glycan structures is defined, mutant glycosylation patterns, overview. The presence of a stereotypic, conserved deletion mutation is inactivating pglH in strains of Neisseria gonorrhoeae, Neisseria meningitidis, and related commensals | Neisseria gonorrhoeae |
additional information | replacement of the strain N400 locus carrying the deleted form of ORFs 2 and 3 with those derived from the Neisseria gonorrhoeae strain FA1090. The presence of a stereotypic, conserved deletion mutation is inactivating pglH in strains of Neisseria gonorrhoeae, Neisseria meningitidis, and related commensals | Neisseria meningitidis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3 UDP-N-acetyl-alpha-D-galactosamine + GalNAc-alpha-(1->4)-GalNAc-alpha-(1->3)-Bac2,4diNAc-diphospho-tritrans,heptacis-undecaprenol | Neisseria gonorrhoeae | - |
3 UDP + [GalNAc-alpha-(1->4)]4-GalNAc-alpha-(1->3)-Bac2,4diNAc-diphospho-tritrans,heptacis-undecaprenol | - |
? | |
3 UDP-N-acetyl-alpha-D-galactosamine + GalNAc-alpha-(1->4)-GalNAc-alpha-(1->3)-Bac2,4diNAc-diphospho-tritrans,heptacis-undecaprenol | Neisseria meningitidis | - |
3 UDP + [GalNAc-alpha-(1->4)]4-GalNAc-alpha-(1->3)-Bac2,4diNAc-diphospho-tritrans,heptacis-undecaprenol | - |
? | |
additional information | Neisseria meningitidis | PglH (from ORF3) acts as a glucosyltransferase, which uses both undecaprenyl-diphospho-linked Bac2,4diNAc and glyceramido-acetamido trideoxyhexose, GATDH, glycosyltransferase specificity of PglH, overview. Recombinantly expressed PglH from strain Z2491 specifically transfers UDP-Glc to undecaprenyldiphospho-Bac2,4diNAc. PglH shows no transferase activity in the presence of UDP-Gal, UDP-GlcNAc, UDPGalNAc, and GDP-Man | ? | - |
? | |
additional information | Neisseria gonorrhoeae | PglH (from ORF3) acts as a glucosyltransferase, which uses both UndPP-linked diNAcBac and glyceramido-acetamido trideoxyhexose, GATDH | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Neisseria gonorrhoeae | - |
strains FA1090 and N400, gene pglH | - |
Neisseria meningitidis | - |
strains FAM18 and Z2491, gene pglH | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3 UDP-N-acetyl-alpha-D-galactosamine + GalNAc-alpha-(1->4)-GalNAc-alpha-(1->3)-Bac2,4diNAc-diphospho-tritrans,heptacis-undecaprenol | - |
Neisseria gonorrhoeae | 3 UDP + [GalNAc-alpha-(1->4)]4-GalNAc-alpha-(1->3)-Bac2,4diNAc-diphospho-tritrans,heptacis-undecaprenol | - |
? | |
3 UDP-N-acetyl-alpha-D-galactosamine + GalNAc-alpha-(1->4)-GalNAc-alpha-(1->3)-Bac2,4diNAc-diphospho-tritrans,heptacis-undecaprenol | - |
Neisseria meningitidis | 3 UDP + [GalNAc-alpha-(1->4)]4-GalNAc-alpha-(1->3)-Bac2,4diNAc-diphospho-tritrans,heptacis-undecaprenol | - |
? | |
additional information | PglH (from ORF3) acts as a glucosyltransferase, which uses both undecaprenyl-diphospho-linked Bac2,4diNAc and glyceramido-acetamido trideoxyhexose, GATDH, glycosyltransferase specificity of PglH, overview. Recombinantly expressed PglH from strain Z2491 specifically transfers UDP-Glc to undecaprenyldiphospho-Bac2,4diNAc. PglH shows no transferase activity in the presence of UDP-Gal, UDP-GlcNAc, UDPGalNAc, and GDP-Man | Neisseria meningitidis | ? | - |
? | |
additional information | PglH (from ORF3) acts as a glucosyltransferase, which uses both UndPP-linked diNAcBac and glyceramido-acetamido trideoxyhexose, GATDH | Neisseria gonorrhoeae | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PglH | - |
Neisseria gonorrhoeae |
PglH | - |
Neisseria meningitidis |
General Information | Comment | Organism |
---|---|---|
malfunction | polymorphisms in PglH are associated with diminished glycosyltransferase activity | Neisseria meningitidis |
additional information | Pgl genotypes and phenotypes of strains, overview | Neisseria meningitidis |
additional information | Pgl genotypes and phenotypes of strains, overview. Epistatic interactions involving pglH at the intragenic and intergenic levels | Neisseria gonorrhoeae |
physiological function | metabolic conflict related to competition for a shared substrate between the opposing glycosyltransferases PglA and PglH | Neisseria meningitidis |
physiological function | metabolic conflict related to competition for a shared substrate between the opposing glycosyltransferases PglA and PglH. Protein-linked PglH-derived disaccharides are immunogenic and antigenic, both diNAcBac- and GATDH-based PglH-derived glycans act as glycosyl donors in general, broad-spectrum protein glycosylation | Neisseria gonorrhoeae |