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Literature summary for 2.4.1.291 extracted from

  • Chen, M.M.; Weerapana, E.; Ciepichal, E.; Stupak, J.; Reid, C.W.; Swiezewska, E.; Imperiali, B.
    Polyisoprenol specificity in the Campylobacter jejuni N-linked glycosylation pathway (2007), Biochemistry, 46, 14342-14348.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.1.291

Cloned(Commentary)

Cloned (Comment) Organism
gene pglJ, expression of His-tagged PglJ in Escherichia coli Campylobacter jejuni

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics and substrate specificity, overview Campylobacter jejuni

Localization

Localization Comment Organism GeneOntology No. Textmining
plasma membrane associated, the enzyme functions at a membrane interface on the cytoplasmic side Campylobacter jejuni 5886
-

Organism

Organism UniProt Comment Textmining
Campylobacter jejuni
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged PglJ from Escherichia coli by nickel affinity chromatography and gel filtration Campylobacter jejuni

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information with polyisoprenols substrates of varying length, the enzyme exhibits specificity with respect to cis-double bond geometry and alpha-unsaturation of the native substrate are important features, while the precise polyisoprene length may be less critical. PglJ accepts the prenol-9 substrate with efficiencies equal to that of the native undecaprenol-based substrate Campylobacter jejuni ?
-
 ?
UDP-N-acetyl-alpha-D-galactosamine + N-acetyl-D-galactosaminyl-alpha-(1->3)-N,N'-diacetyl-alpha-D-bacillosaminyl-diphospho-tritrans,heptacis-undecaprenol in the assay, radiolabeled UDP-GalNAc is used with radiolabeled polyisoprenyl diphosphate Bac2,4diNAc-[3H]GalNAc, enzymatically synthesized Campylobacter jejuni UDP + N-acetyl-D-galactosaminyl-alpha-(1->4)-N-acetyl-D-galactosaminyl-alpha-(1->3)-N,N'-diacetyl-alpha-D-bacillosaminyl-diphospho-tritrans,heptacis-undecaprenol
-
 ?

Synonyms

Synonyms Comment Organism
PglJ
-
 Campylobacter jejuni

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
 assay at Campylobacter jejuni

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Campylobacter jejuni

General Information

General Information Comment Organism
physiological function the enzyme is involved in a general N-linked glycosylation pathway in which a heptasaccharide is sequentially assembled onto a polyisoprenyl diphosphate carrier, anchored at the cytoplasmic face of the inner membrane, and subsequently transferred to the asparagine side chain of an acceptor protein. The polyisoprenyl carrier plays a specific role in the function of these enzymes beyond a purely physical role as a membrane anchor Campylobacter jejuni