Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.4.1.289 extracted from

  • Glover, K.J.; Weerapana, E.; Imperiali, B.
    In vitro assembly of the undecaprenylpyrophosphate-linked heptasaccharide for prokaryotic N-linked glycosylation (2005), Proc. Natl. Acad. Sci. USA, 102, 14255-14259.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.1.289

Cloned(Commentary)

Cloned (Comment) Organism
gene pglH, overexpression of His-tagged PglA in Escherichia coli Campylobacter jejuni

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
 x * 43000, recombinant His-tagged PglA, SDS-PAGE Campylobacter jejuni

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dTDP-6-deoxy-beta-L-mannose + N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol Campylobacter jejuni
-
 dTDP + alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol
-
 ?
dTDP-6-deoxy-beta-L-mannose + N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol Campylobacter jejuni NCTC 11168
-
 dTDP + alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol
-
 ?

Organism

Organism UniProt Comment Textmining
Campylobacter jejuni
-
 gene pglA, pgl gene cluster
-
Campylobacter jejuni NCTC 11168
-
 gene pglA, pgl gene cluster
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged PglA from Escherichia coli by nickel affinity chromatography Campylobacter jejuni

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dTDP-6-deoxy-beta-L-mannose + N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol
-
 Campylobacter jejuni dTDP + alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol
-
 ?
dTDP-6-deoxy-beta-L-mannose + N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol
-
 Campylobacter jejuni NCTC 11168 dTDP + alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol
-
 ?
additional information in vitro, trisaccharide is prepared by using PglA and PglJ and then reacts singly with PglH Campylobacter jejuni ?
-
 ?
additional information in vitro, trisaccharide is prepared by using PglA and PglJ and then reacts singly with PglH Campylobacter jejuni NCTC 11168 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 43000, recombinant His-tagged PglA, SDS-PAGE Campylobacter jejuni

Synonyms

Synonyms Comment Organism
PglA
-
 Campylobacter jejuni

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8 assay at Campylobacter jejuni

General Information

General Information Comment Organism
metabolism PglA is part of a general N-linked glycosylation pathway, encoded by the pgl gene cluster, which culminates in the transfer of a heptasaccharide: GalNAc-alpha1,4-GalNAc-alpha1,4-(Glcbeta1,3)-GalNAc-alpha1,4-GalNAc-alpha1,4-GalNAc-alpha1,3-Bac, where Bac is bacillosamine (2,4-diacetamido-2,4,6-trideoxyglucose), from a membrane-anchored undecaprenyldiphosphate-linked donor to the asparagine side chain of proteins at the Asn-X-Ser/Thr motif. The glycosyltransferases, PglA, PglH, PglI, and PglJ, responsible for the biosynthesis of the Und-PP-linked heptasaccharide. PglA adds the first GalNAc residue on to the isoprenoid-linked Bac carrier Campylobacter jejuni