Data extracted from this reference:
Engineering
D256A
the mutant shows almost no activity even in the presence of Mn2+ or Mg2+
Mycobacterium tuberculosis
D256E
the mutant shows almost no activity even in the presence of Mn2+ or Mg2+
Mycobacterium tuberculosis
D258A
the mutant shows almost no activity in the presence of Mg2+, but wild type activity in the presence of Mn2+
Mycobacterium tuberculosis
D258E
the mutant shows almost no activity in the presence of Mg2+, and about 10% activity in the presence of Mn2+ as compared to the wild type enzyme
Mycobacterium tuberculosis
D371A
inactive
Mycobacterium tuberculosis
D372A
inactive
Mycobacterium tuberculosis
D372E
inactive
Mycobacterium tuberculosis
Inhibitors
EDTA
Mycobacterium tuberculosis
Metals/Ions
Mg2+
essential for catalysis
Mycobacterium tuberculosis
Mn2+
essential for catalysis
Mycobacterium tuberculosis
Organism
Mycobacterium tuberculosis
Mycobacterium tuberculosis H37Rv
Substrates and Products (Substrate)
UDP-alpha-D-galactofuranose + beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol
718920
Mycobacterium tuberculosis
UDP + [beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->6)]14-beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol
?
UDP-alpha-D-galactofuranose + beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol
718920
Mycobacterium tuberculosis H37Rv
UDP + [beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->6)]14-beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol
?
Synonyms
GlfT2
Mycobacterium tuberculosis
Rv3808c
Mycobacterium tuberculosis
Engineering (protein specific)
D256A
the mutant shows almost no activity even in the presence of Mn2+ or Mg2+
Mycobacterium tuberculosis
D256E
the mutant shows almost no activity even in the presence of Mn2+ or Mg2+
Mycobacterium tuberculosis
D258A
the mutant shows almost no activity in the presence of Mg2+, but wild type activity in the presence of Mn2+
Mycobacterium tuberculosis
D258E
the mutant shows almost no activity in the presence of Mg2+, and about 10% activity in the presence of Mn2+ as compared to the wild type enzyme
Mycobacterium tuberculosis
D371A
inactive
Mycobacterium tuberculosis
D372A
inactive
Mycobacterium tuberculosis
D372E
inactive
Mycobacterium tuberculosis
Inhibitors (protein specific)
EDTA
Mycobacterium tuberculosis
Metals/Ions (protein specific)
Mg2+
essential for catalysis
Mycobacterium tuberculosis
Mn2+
essential for catalysis
Mycobacterium tuberculosis
Substrates and Products (Substrate) (protein specific)
UDP-alpha-D-galactofuranose + beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol
718920
Mycobacterium tuberculosis
UDP + [beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->6)]14-beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol
?
UDP-alpha-D-galactofuranose + beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol
718920
Mycobacterium tuberculosis H37Rv
UDP + [beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->6)]14-beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol
?
General Information
metabolism
GlfT2 catalyzes the formation of mycobacterial galactan, mediating galactan polymerization using only one active site that manifests dual regioselectivity
Mycobacterium tuberculosis
General Information (protein specific)
metabolism
GlfT2 catalyzes the formation of mycobacterial galactan, mediating galactan polymerization using only one active site that manifests dual regioselectivity
Mycobacterium tuberculosis
Other publictions for EC 2.4.1.288
735959
Dumitrescu
Selectfluor and NFSI exo-glyca ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis ATCC 25618
Chemistry
20
15208-15215
2014
-
-
-
-
-
-
2
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
2
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
736729
Li
-
Sulfonium ions as inhibitors o ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis ATCC 25618
MedChemComm
5
1130-1137
2014
-
-
-
-
-
-
2
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
718920
May
A processive carbohydrate poly ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
Biochemistry
51
1148-1159
2012
-
-
-
-
7
-
1
-
-
2
-
-
-
179
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
7
-
-
1
-
-
-
2
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
719665
Brown
Fluorosugar chain termination ...
Mycobacterium tuberculosis
J. Am. Chem. Soc.
134
6552-6555
2012
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
720037
Wheatley
Tetrameric structure of the Gl ...
Mycobacterium tuberculosis
J. Biol. Chem.
287
28132-28143
2012
-
-
1
1
5
-
-
8
-
1
-
-
-
1
-
-
1
-
-
-
-
-
2
1
3
-
-
-
8
-
-
-
-
-
-
-
-
-
1
-
1
5
-
-
-
-
8
-
1
-
-
-
-
-
1
-
-
-
-
2
1
-
-
-
8
-
-
-
-
-
1
1
-
-
-
720611
Poulin
Synthetic UDP-galactofuranose ...
Mycobacterium tuberculosis
Org. Biomol. Chem.
10
4074-4087
2012
-
-
-
-
-
-
6
7
-
-
-
-
-
1
-
-
-
-
-
-
13
-
14
-
1
-
-
-
7
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
6
1
7
-
-
-
-
-
-
-
-
-
-
13
-
14
-
-
-
-
7
-
-
-
-
-
-
-
-
7
7
719284
Szczepina
STD-NMR studies suggest that t ...
Mycobacterium tuberculosis
ChemBioChem
10
2052-2059
2009
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
3
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
720894
May
A tethering mechanism for leng ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
Proc. Natl. Acad. Sci. USA
106
11851-11856
2009
-
-
1
-
-
-
-
-
-
-
-
-
-
181
-
-
1
-
-
-
-
-
12
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
12
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
719218
Rose
Development of a coupled spect ...
Mycobacterium tuberculosis
Carbohydr. Res.
343
2130-2139
2008
-
-
1
-
-
-
-
1
-
-
1
-
-
2
-
-
1
-
-
-
-
2
3
1
2
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
1
-
-
-
2
3
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
719712
Belanova
Galactosyl transferases in myc ...
Mycolicibacterium smegmatis
J. Bacteriol.
190
1141-1145
2008
-
-
1
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
3
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
719707
Mikusova
Identification of a novel gala ...
Mycobacterium tuberculosis
J. Bacteriol.
188
6592-6598
2006
-
-
-
-
-
-
-
-
-
-
1
-
-
4
-
-
-
-
-
-
-
-
2
1
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-