A processive carbohydrate polymerase that mediates bifunctional catalysis using a single active site
May, J.F.; Levengood, M.R.; Splain, R.A.; Brown, C.D.; Kiessling, L.L.; Biochemistry 51, 1148-1159 (2012) 
Data extracted from this reference:
Engineering
Protein Variants
Commentary
Organism
D256A
the mutant shows almost no activity even in the presence of Mn2+ or Mg2+
Mycobacterium tuberculosis
D256E
the mutant shows almost no activity even in the presence of Mn2+ or Mg2+
Mycobacterium tuberculosis
D258A
the mutant shows almost no activity in the presence of Mg2+, but wild type activity in the presence of Mn2+
Mycobacterium tuberculosis
D258E
the mutant shows almost no activity in the presence of Mg2+, and about 10% activity in the presence of Mn2+ as compared to the wild type enzyme
Mycobacterium tuberculosis
D371A
inactive
Mycobacterium tuberculosis
D372A
inactive
Mycobacterium tuberculosis
D372E
inactive
Mycobacterium tuberculosis
Inhibitors
Inhibitors
Commentary
Organism
Structure
EDTA
-
Mycobacterium tuberculosis
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
essential for catalysis
Mycobacterium tuberculosis
Mn2+
essential for catalysis
Mycobacterium tuberculosis
Organism
Organism
UniProt
Commentary
Textmining
Mycobacterium tuberculosis
-
-
-
Mycobacterium tuberculosis H37Rv
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
UDP-alpha-D-galactofuranose + beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol
-
718920
Mycobacterium tuberculosis
UDP + [beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->6)]14-beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol
-
-
-
?
UDP-alpha-D-galactofuranose + beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol
-
718920
Mycobacterium tuberculosis H37Rv
UDP + [beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->6)]14-beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
GlfT2
-
Mycobacterium tuberculosis
Rv3808c
-
Mycobacterium tuberculosis
Engineering (protein specific)
Protein Variants
Commentary
Organism
D256A
the mutant shows almost no activity even in the presence of Mn2+ or Mg2+
Mycobacterium tuberculosis
D256E
the mutant shows almost no activity even in the presence of Mn2+ or Mg2+
Mycobacterium tuberculosis
D258A
the mutant shows almost no activity in the presence of Mg2+, but wild type activity in the presence of Mn2+
Mycobacterium tuberculosis
D258E
the mutant shows almost no activity in the presence of Mg2+, and about 10% activity in the presence of Mn2+ as compared to the wild type enzyme
Mycobacterium tuberculosis
D371A
inactive
Mycobacterium tuberculosis
D372A
inactive
Mycobacterium tuberculosis
D372E
inactive
Mycobacterium tuberculosis
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
EDTA
-
Mycobacterium tuberculosis
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
essential for catalysis
Mycobacterium tuberculosis
Mn2+
essential for catalysis
Mycobacterium tuberculosis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
UDP-alpha-D-galactofuranose + beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol
-
718920
Mycobacterium tuberculosis
UDP + [beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->6)]14-beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol
-
-
-
?
UDP-alpha-D-galactofuranose + beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol
-
718920
Mycobacterium tuberculosis H37Rv
UDP + [beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->6)]14-beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol
-
-
-
?
General Information
General Information
Commentary
Organism
metabolism
GlfT2 catalyzes the formation of mycobacterial galactan, mediating galactan polymerization using only one active site that manifests dual regioselectivity
Mycobacterium tuberculosis
General Information (protein specific)
General Information
Commentary
Organism
metabolism
GlfT2 catalyzes the formation of mycobacterial galactan, mediating galactan polymerization using only one active site that manifests dual regioselectivity
Mycobacterium tuberculosis
Other publictions for EC 2.4.1.288
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
735959
Dumitrescu
Selectfluor and NFSI exo-glyca ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis ATCC 25618
Chemistry
20
15208-15215
2014
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2
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3
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1
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2
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2
2
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736729
Li
-
Sulfonium ions as inhibitors o ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis ATCC 25618
MedChemComm
5
1130-1137
2014
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2
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3
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2
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-
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-
-
-
-
-
-
-
-
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-
718920
May
A processive carbohydrate poly ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
Biochemistry
51
1148-1159
2012
-
-
-
-
7
-
1
-
-
2
-
-
-
172
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2
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2
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7
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1
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2
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2
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-
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-
1
1
-
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-
719665
Brown
Fluorosugar chain termination ...
Mycobacterium tuberculosis
J. Am. Chem. Soc.
134
6552-6555
2012
-
-
-
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2
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2
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1
1
-
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720037
Wheatley
Tetrameric structure of the Gl ...
Mycobacterium tuberculosis
J. Biol. Chem.
287
28132-28143
2012
-
-
1
1
5
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-
7
-
1
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1
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1
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2
1
3
-
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8
-
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-
1
-
1
5
-
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-
7
-
1
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-
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-
-
1
-
-
-
-
2
1
-
-
-
8
-
-
-
-
-
1
1
-
-
-
720611
Poulin
Synthetic UDP-galactofuranose ...
Mycobacterium tuberculosis
Org. Biomol. Chem.
10
4074-4087
2012
-
-
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-
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6
7
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1
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11
-
14
-
1
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7
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1
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-
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6
1
7
-
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11
-
14
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-
7
-
-
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-
7
7
719284
Szczepina
STD-NMR studies suggest that t ...
Mycobacterium tuberculosis
ChemBioChem
10
2052-2059
2009
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2
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3
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720894
May
A tethering mechanism for leng ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
Proc. Natl. Acad. Sci. USA
106
11851-11856
2009
-
-
1
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-
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173
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1
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12
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3
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1
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1
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12
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719218
Rose
Development of a coupled spect ...
Mycobacterium tuberculosis
Carbohydr. Res.
343
2130-2139
2008
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1
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1
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1
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2
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1
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2
3
1
2
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1
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1
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1
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1
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1
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2
3
1
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1
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719712
Belanova
Galactosyl transferases in myc ...
Mycolicibacterium smegmatis
J. Bacteriol.
190
1141-1145
2008
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1
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4
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3
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3
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1
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3
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1
1
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719707
Mikusova
Identification of a novel gala ...
Mycobacterium tuberculosis
J. Bacteriol.
188
6592-6598
2006
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1
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3
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2
1
4
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1
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1
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1
1
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