BRENDA - Enzyme Database
show all sequences of 2.4.1.284

Discovery of parallel pathways of kanamycin biosynthesis allows antibiotic manipulation

Park, J.; Park, S.; Nepal, K.; Han, A.; Ban, Y.; Yoo, Y.; Kim, E.; Kim, E.; Kim, D.; Sohng, J.; Yoon, Y.; Nat. Chem. Biol. 7, 843-852 (2011)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
KanF cannot be functionally expressed in Escherichia coli
Streptomyces kanamyceticus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.28
-
2-deoxystreptamine
using UDP-D-glucose as donor, in 100 mM Tris-HCl (pH 7.6), 10 mM MgCl2, 6 mM 2-mercaptoethanol, and 1 mM phenylmethylsulfonyl fluoride, at 30°C
Streptomyces kanamyceticus
0.32
-
2-deoxystreptamine
using UDP-N-acetyl-alpha-D-glucosamine as donor, in 100 mM Tris-HCl (pH 7.6), 10 mM MgCl2, 6 mM 2-mercaptoethanol, and 1 mM phenylmethylsulfonyl fluoride, at 30°C
Streptomyces kanamyceticus
0.44
-
UDP-D-glucose
in 100 mM Tris-HCl (pH 7.6), 10 mM MgCl22, 6 mM 2-mercaptoethanol, and 1 mM phenylmethylsulfonyl fluoride, at 30°C
Streptomyces kanamyceticus
2.54
-
UDP-N-acetyl-alpha-D-glucosamine
in 100 mM Tris-HCl (pH 7.6), 10 mM MgCl2, 6 mM 2-mercaptoethanol, and 1 mM phenylmethylsulfonyl fluoride, at 30°C
Streptomyces kanamyceticus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Streptomyces kanamyceticus
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
UDP-D-glucose + 2-deoxystreptamine
best substrate
720518
Streptomyces kanamyceticus
UDP + 2'-deamino-2'-hydroxyparomamine
-
-
-
?
UDP-N-acetyl-alpha-D-glucosamine + 2-deoxystreptamine
reaction of 2.4.1.283, one fifth of the catalytic efficiency with UDP-D-glucose
720518
Streptomyces kanamyceticus
UDP + 2'-N-acetylparomamine
-
-
-
?
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.29
-
UDP-D-glucose
in 100 mM Tris-HCl (pH 7.6), 10 mM MgCl2, 6 mM 2-mercaptoethanol, and 1 mM phenylmethylsulfonyl fluoride, at 30°C
Streptomyces kanamyceticus
0.33
-
UDP-N-acetyl-alpha-D-glucosamine
in 100 mM Tris-HCl (pH 7.6), 10 mM MgCl2, 6 mM 2-mercaptoethanol, and 1 mM phenylmethylsulfonyl fluoride, at 30°C
Streptomyces kanamyceticus
Cloned(Commentary) (protein specific)
Commentary
Organism
KanF cannot be functionally expressed in Escherichia coli
Streptomyces kanamyceticus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.28
-
2-deoxystreptamine
using UDP-D-glucose as donor, in 100 mM Tris-HCl (pH 7.6), 10 mM MgCl2, 6 mM 2-mercaptoethanol, and 1 mM phenylmethylsulfonyl fluoride, at 30°C
Streptomyces kanamyceticus
0.32
-
2-deoxystreptamine
using UDP-N-acetyl-alpha-D-glucosamine as donor, in 100 mM Tris-HCl (pH 7.6), 10 mM MgCl2, 6 mM 2-mercaptoethanol, and 1 mM phenylmethylsulfonyl fluoride, at 30°C
Streptomyces kanamyceticus
0.44
-
UDP-D-glucose
in 100 mM Tris-HCl (pH 7.6), 10 mM MgCl22, 6 mM 2-mercaptoethanol, and 1 mM phenylmethylsulfonyl fluoride, at 30°C
Streptomyces kanamyceticus
2.54
-
UDP-N-acetyl-alpha-D-glucosamine
in 100 mM Tris-HCl (pH 7.6), 10 mM MgCl2, 6 mM 2-mercaptoethanol, and 1 mM phenylmethylsulfonyl fluoride, at 30°C
Streptomyces kanamyceticus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
UDP-D-glucose + 2-deoxystreptamine
best substrate
720518
Streptomyces kanamyceticus
UDP + 2'-deamino-2'-hydroxyparomamine
-
-
-
?
UDP-N-acetyl-alpha-D-glucosamine + 2-deoxystreptamine
reaction of 2.4.1.283, one fifth of the catalytic efficiency with UDP-D-glucose
720518
Streptomyces kanamyceticus
UDP + 2'-N-acetylparomamine
-
-
-
?
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.29
-
UDP-D-glucose
in 100 mM Tris-HCl (pH 7.6), 10 mM MgCl2, 6 mM 2-mercaptoethanol, and 1 mM phenylmethylsulfonyl fluoride, at 30°C
Streptomyces kanamyceticus
0.33
-
UDP-N-acetyl-alpha-D-glucosamine
in 100 mM Tris-HCl (pH 7.6), 10 mM MgCl2, 6 mM 2-mercaptoethanol, and 1 mM phenylmethylsulfonyl fluoride, at 30°C
Streptomyces kanamyceticus
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.13
-
UDP-N-acetyl-alpha-D-glucosamine
in 100 mM Tris-HCl (pH 7.6), 10 mM MgCl2, 6 mM 2-mercaptoethanol, and 1 mM phenylmethylsulfonyl fluoride, at 30°C
Streptomyces kanamyceticus
0.66
-
UDP-D-glucose
in 100 mM Tris-HCl (pH 7.6), 10 mM MgCl2, 6 mM 2-mercaptoethanol, and 1 mM phenylmethylsulfonyl fluoride, at 30°C
Streptomyces kanamyceticus
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.13
-
UDP-N-acetyl-alpha-D-glucosamine
in 100 mM Tris-HCl (pH 7.6), 10 mM MgCl2, 6 mM 2-mercaptoethanol, and 1 mM phenylmethylsulfonyl fluoride, at 30°C
Streptomyces kanamyceticus
0.66
-
UDP-D-glucose
in 100 mM Tris-HCl (pH 7.6), 10 mM MgCl2, 6 mM 2-mercaptoethanol, and 1 mM phenylmethylsulfonyl fluoride, at 30°C
Streptomyces kanamyceticus
Other publictions for EC 2.4.1.284
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
720518
Park
Discovery of parallel pathways ...
Streptomyces kanamyceticus
Nat. Chem. Biol.
7
843-852
2011
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1
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4
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1
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2
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2
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1
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4
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2
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2
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2
2
719134
Park
Expanding substrate specificit ...
Streptomyces kanamyceticus, Streptomyces kanamyceticus ATCC 12853
Biotechnol. Bioeng.
102
988-994
2009
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1
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4
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8
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1
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8
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720207
Zhao
-
Genetic organization of a 50-k ...
Streptomyces kanamyceticus, Streptomyces kanamyceticus ATCC 12853
J. Microbiol. Biotechnol.
15
346-353
2005
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4
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