BRENDA - Enzyme Database show
show all sequences of 2.4.1.284

Expanding substrate specificity of GT-B fold glycosyltransferase via domain swapping and high-throughput screening

Park, S.H.; Park, H.Y.; Sohng, J.K.; Lee, H.C.; Liou, K.; Yoon, Y.J.; Kim, B.G.; Biotechnol. Bioeng. 102, 988-994 (2009)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Streptomyces kanamyceticus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Streptomyces kanamyceticus
-
-
-
Streptomyces kanamyceticus ATCC 12853
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
dTDP-D-glucose + 2-deoxystreptamine
100% activity
719134
Streptomyces kanamyceticus
?
-
-
-
?
dTDP-D-glucose + 2-deoxystreptamine
100% activity
719134
Streptomyces kanamyceticus ATCC 12853
?
-
-
-
?
GDP-D-mannose + 2-deoxystreptamine
highest activity (about 200% compared to dTDP-D-glucose)
719134
Streptomyces kanamyceticus
?
-
-
-
?
GDP-D-mannose + 2-deoxystreptamine
highest activity (about 200% compared to dTDP-D-glucose)
719134
Streptomyces kanamyceticus ATCC 12853
?
-
-
-
?
additional information
wild type KanF shows weak glucosylation activity for 2-deoxystreptamine and low activity towards UDP-D-glucose, dTDP-L-rhamnose, dTDP-4-keto-6-Ddeoxy-glucose, dTDP-D-fucosamine, and dTDP-2-deoxy-D-glucose
719134
Streptomyces kanamyceticus
?
-
-
-
-
additional information
wild type KanF shows weak glucosylation activity for 2-deoxystreptamine and low activity towards UDP-D-glucose, dTDP-L-rhamnose, dTDP-4-keto-6-Ddeoxy-glucose, dTDP-D-fucosamine, and dTDP-2-deoxy-D-glucose
719134
Streptomyces kanamyceticus ATCC 12853
?
-
-
-
-
UDP-D-galactose + 2-deoxystreptamine
about 50% activity compared to dTDP-D-glucose
719134
Streptomyces kanamyceticus
?
-
-
-
?
UDP-D-galactose + 2-deoxystreptamine
about 50% activity compared to dTDP-D-glucose
719134
Streptomyces kanamyceticus ATCC 12853
?
-
-
-
?
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Streptomyces kanamyceticus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
dTDP-D-glucose + 2-deoxystreptamine
100% activity
719134
Streptomyces kanamyceticus
?
-
-
-
?
dTDP-D-glucose + 2-deoxystreptamine
100% activity
719134
Streptomyces kanamyceticus ATCC 12853
?
-
-
-
?
GDP-D-mannose + 2-deoxystreptamine
highest activity (about 200% compared to dTDP-D-glucose)
719134
Streptomyces kanamyceticus
?
-
-
-
?
GDP-D-mannose + 2-deoxystreptamine
highest activity (about 200% compared to dTDP-D-glucose)
719134
Streptomyces kanamyceticus ATCC 12853
?
-
-
-
?
additional information
wild type KanF shows weak glucosylation activity for 2-deoxystreptamine and low activity towards UDP-D-glucose, dTDP-L-rhamnose, dTDP-4-keto-6-Ddeoxy-glucose, dTDP-D-fucosamine, and dTDP-2-deoxy-D-glucose
719134
Streptomyces kanamyceticus
?
-
-
-
-
additional information
wild type KanF shows weak glucosylation activity for 2-deoxystreptamine and low activity towards UDP-D-glucose, dTDP-L-rhamnose, dTDP-4-keto-6-Ddeoxy-glucose, dTDP-D-fucosamine, and dTDP-2-deoxy-D-glucose
719134
Streptomyces kanamyceticus ATCC 12853
?
-
-
-
-
UDP-D-galactose + 2-deoxystreptamine
about 50% activity compared to dTDP-D-glucose
719134
Streptomyces kanamyceticus
?
-
-
-
?
UDP-D-galactose + 2-deoxystreptamine
about 50% activity compared to dTDP-D-glucose
719134
Streptomyces kanamyceticus ATCC 12853
?
-
-
-
?
Other publictions for EC 2.4.1.284
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
720518
Park
Discovery of parallel pathways ...
Streptomyces kanamyceticus
Nat. Chem. Biol.
7
843-852
2011
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1
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4
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1
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2
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2
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1
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4
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2
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2
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2
2
719134
Park
Expanding substrate specificit ...
Streptomyces kanamyceticus, Streptomyces kanamyceticus ATCC 12853
Biotechnol. Bioeng.
102
988-994
2009
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1
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4
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8
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1
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8
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720207
Zhao
-
Genetic organization of a 50-k ...
Streptomyces kanamyceticus, Streptomyces kanamyceticus ATCC 12853
J. Microbiol. Biotechnol.
15
346-353
2005
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4
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