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Literature summary for 2.4.1.282 extracted from
- 3-O-alpha-D-glucopyranosyl-L-rhamnose posphorylase from Clostridium phytofermentans (2012), Carbohydr. Res., 350, 94-97.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Lachnoclostridium phytofermentans |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.7 | - |
L-rhamnose | pH 6.5, 30°C, reverse phosphorolysis reaction, cosubstrate beta-D-glucose 1-phosphate | Lachnoclostridium phytofermentans |  |
| 9.6 | - |
beta-D-glucose 1-phosphate | pH 6.5, 30°C, reverse phosphorolysis reaction | Lachnoclostridium phytofermentans | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Lachnoclostridium phytofermentans | 5829 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 87990 | - |
calculated from amino acid sequence | Lachnoclostridium phytofermentans |
| 88000 | - |
SDS-PAGE | Lachnoclostridium phytofermentans |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-O-beta-D-mannopyranosyl-D-glucopyranose + phosphate | Lachnoclostridium phytofermentans | phosphorolysis reaction | D-glucose + alpha-D-mannose 1-phosphate | - |
r | |
| 4-O-beta-D-mannopyranosyl-D-glucopyranose + phosphate | Lachnoclostridium phytofermentans ATCC 700394 | phosphorolysis reaction | D-glucose + alpha-D-mannose 1-phosphate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lachnoclostridium phytofermentans | A9KM56 | - |
- |
| Lachnoclostridium phytofermentans ATCC 700394 | A9KM56 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-O-alpha-D-glucopyranosyl-L-rhamnopyranose + phosphate | - |
Lachnoclostridium phytofermentans | L-rhamnose + beta-D-glucose 1-phosphate | - |
r | |
| 3-O-alpha-D-glucopyranosyl-L-rhamnopyranose + phosphate | - |
Lachnoclostridium phytofermentans ATCC 700394 | L-rhamnose + beta-D-glucose 1-phosphate | - |
r | |
| 4-O-beta-D-mannopyranosyl-D-glucopyranose + phosphate | phosphorolysis reaction | Lachnoclostridium phytofermentans | D-glucose + alpha-D-mannose 1-phosphate | - |
r | |
| 4-O-beta-D-mannopyranosyl-D-glucopyranose + phosphate | phosphorolysis reaction | Lachnoclostridium phytofermentans ATCC 700394 | D-glucose + alpha-D-mannose 1-phosphate | - |
r | |
| L-rhamnose + beta-D-glucose 1-phosphate | - |
Lachnoclostridium phytofermentans | 3-O-alpha-D-glucopyranosyl-L-rhamnopyranose + phosphate | - |
r | |
| L-rhamnose + beta-D-glucose 1-phosphate | - |
Lachnoclostridium phytofermentans ATCC 700394 | 3-O-alpha-D-glucopyranosyl-L-rhamnopyranose + phosphate | - |
r | |
| additional information | The enzyme does not phosphorylyze alpha,alpha-trehalose, kojibiose, nigerose, maltose. In the reverse phosphorolysis reaction the enzyme is specific for L-rhamnose as acceptor and beta-D-glucose 1-phosphate as donor. | Lachnoclostridium phytofermentans | ? | - |
? | |
| additional information | The enzyme does not phosphorylyze alpha,alpha-trehalose, kojibiose, nigerose, maltose. In the reverse phosphorolysis reaction the enzyme is specific for L-rhamnose as acceptor and beta-D-glucose 1-phosphate as donor. | Lachnoclostridium phytofermentans ATCC 700394 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| cphy1019 | - |
Lachnoclostridium phytofermentans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Lachnoclostridium phytofermentans |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
stable up to | Lachnoclostridium phytofermentans |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.6 | - |
L-rhamnose | pH 6.5, 30°C, reverse phosphorolysis reaction, cosubstrate beta-D-glucose 1-phosphate | Lachnoclostridium phytofermentans | |
| 2.5 | - |
beta-D-glucose 1-phosphate | pH 6.5, 30°C, reverse phosphorolysis reaction | Lachnoclostridium phytofermentans | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
- |
Lachnoclostridium phytofermentans |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9 | - |
Lachnoclostridium phytofermentans |
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