Literature summary for 2.4.1.280 extracted from

Hidaka, M.; Honda, Y.; Kitaoka, M.; Nirasawa, S.; Hayashi, K.; Wakagi, T.; Shoun, H.; Fushinobu, S.
Chitobiose phosphorylase from Vibrio proteolyticus, a member of glycosyl transferase family 36, has a clan GH-L-like (alpha/alpha)(6) barrel fold (2004), Structure, 12, 937-947.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Vibrio proteolyticus

Crystallization (Commentary)

Crystallization (Comment)	Organism
crystals obtained at 4°C using the hanging-drop vapor interactions diffusion method by mixing 5 microl of a protein solution with 5 microl of a reservoir solution comprising 0.1 M HEPES Na-OH (pH 8.0), 0.17 M CaCl2, and 30%-40% (v/v) PEG400	Vibrio proteolyticus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytosol	-	Vibrio proteolyticus	5829	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
N,N'-diacetylchitobiose + phosphate	Vibrio proteolyticus	-	N-acetyl-D-glucosamine + N-acetyl-alpha-D-glucosamine 1-phosphate	-	r
N,N'-diacetylchitobiose + phosphate	Vibrio proteolyticus	the enzymatic phosphorolysis of ChBP begins with the direct nucleophilic attack to the glycosidic bond with the aid of D492, which donates a proton to the glycosidic oxygen atom, and then proceeds through an oxocarbenium cation activated by ion-like transition state	N-acetyl-D-glucosamine + N-acetyl-alpha-D-glucosamine 1-phosphate	-	r

Organism

Organism	UniProt	Comment	Textmining
Vibrio proteolyticus	Q76IQ9	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Vibrio proteolyticus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
N,N'-diacetylchitobiose + phosphate	-	Vibrio proteolyticus	N-acetyl-D-glucosamine + N-acetyl-alpha-D-glucosamine 1-phosphate	-	r
N,N'-diacetylchitobiose + phosphate	the enzymatic phosphorolysis of ChBP begins with the direct nucleophilic attack to the glycosidic bond with the aid of D492, which donates a proton to the glycosidic oxygen atom, and then proceeds through an oxocarbenium cation activated by ion-like transition state	Vibrio proteolyticus	N-acetyl-D-glucosamine + N-acetyl-alpha-D-glucosamine 1-phosphate	-	r

Synonyms

Synonyms	Comment	Organism
chbP	-	Vibrio proteolyticus
chitobiose phosphorylase	-	Vibrio proteolyticus

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Release 2023.1 (January 2023)