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Literature summary for 2.4.1.28 extracted from
- Glucosylation of deoxyribonucleic acid by enzymes from bacteriophage-infected Escherichia coli (1961), J. Biol. Chem., 236, 1487-1493.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| sulfhydryl reagents | required | Enterobacteria phage T6 |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required for activity | Enterobacteria phage T6 | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-glucose + glucosylhydroxymethylcytosine residue in DNA | Enterobacteria phage T6 | - |
UDP + beta-D-glucosyl-beta-D-glucosyl-hydroxymethylcytosine containing DNA | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Enterobacteria phage T6 | - |
Escherichia coli infected with | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial, using streptomycin sulfate treatment and fractionation on DEAE-cellulose | Enterobacteria phage T6 |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0017 | - |
crude extract | Enterobacteria phage T6 |
| 0.0067 | - |
purified enzyme | Enterobacteria phage T6 |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-glucose + glucosyl-5-hydroxymethylcytosine residue in DNA | no transfer of glucose to synthetic 5-hydroxymethylcytosine-DNA or to T6 DNA, enzyme glucosylates glucosylhydroxymethylcytosine residues of either alpha or beta configuration | Enterobacteria phage T6 | UDP + beta-D-glucosyl-beta-D-glucosyl-5-hydroxymethylcytosine containing DNA | - |
? | |
| UDP-glucose + glucosylhydroxymethylcytosine residue in DNA | - |
Enterobacteria phage T6 | UDP + beta-D-glucosyl-beta-D-glucosyl-hydroxymethylcytosine containing DNA | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Enterobacteria phage T6 |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Enterobacteria phage T6 |
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Release 2023.1 (January 2023)
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