BRENDA - Enzyme Database
show all sequences of 2.4.1.279

Characterization of two phosphorylases for alpha-1,3-oligoglucans from Clostridium phytofermentans

Nihira, T.; Nishimoto, M.; Nakai, H.; Ohtsubo, K.; Kitaoka, M.; J. Appl. Glycosci. 61, 59-66 (2014)
No PubMed abstract available

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expressed in Escherichia coli BL21
Lachnoclostridium phytofermentans
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.49
-
phosphate
pH 7.0, 30C
Lachnoclostridium phytofermentans
1
7
1,5-anhydro-D-glucitol
pH 7.0, 30C, reverse phosphorolysis, donor beta-D-glucopyranose 1-phosphate
Lachnoclostridium phytofermentans
1
7
D-xylose
pH 7.0, 30C, reverse phosphorolysis, donor beta-D-glucopyranose 1-phosphate
Lachnoclostridium phytofermentans
1.2
-
beta-D-glucopyranose 1-phosphate
pH 7.0, 30C, reverse phosphorolysis, acceptor D-glucose
Lachnoclostridium phytofermentans
2.1
-
D-glucose
pH 7.0, 30C, reverse phosphorolysis, donor beta-D-glucopyranose 1-phosphate
Lachnoclostridium phytofermentans
20
-
D-galactose
pH 7.0, 30C, reverse phosphorolysis, donor beta-D-glucopyranose 1-phosphate
Lachnoclostridium phytofermentans
79
-
methyl alpha-D-glucopyranoside
pH 7.0, 30C, reverse phosphorolysis, donor beta-D-glucopyranose 1-phosphate
Lachnoclostridium phytofermentans
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
-
Lachnoclostridium phytofermentans
5829
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate
Lachnoclostridium phytofermentans
-
D-glucose + beta-D-glucopyranose 1-phosphate
-
-
r
3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate
Lachnoclostridium phytofermentans DSM 18823
-
D-glucose + beta-D-glucopyranose 1-phosphate
-
-
r
Organism
Organism
UniProt
Commentary
Textmining
Lachnoclostridium phytofermentans
A9KSL5
-
-
Lachnoclostridium phytofermentans DSM 18823
A9KSL5
-
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Lachnoclostridium phytofermentans
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1,5-anhydro-D-glucitol + beta-D-glucopyranose 1-phosphate
26% activity compared to D-glucose as acceptor
727705
Lachnoclostridium phytofermentans
3-O-alpha-D-glucopyranosyl-1-5-anhydro-D-glucitol + phosphate
-
-
-
r
1,5-anhydro-D-glucitol + beta-D-glucopyranose 1-phosphate
26% activity compared to D-glucose as acceptor
727705
Lachnoclostridium phytofermentans DSM 18823
3-O-alpha-D-glucopyranosyl-1-5-anhydro-D-glucitol + phosphate
-
-
-
r
3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate
-
727705
Lachnoclostridium phytofermentans
D-glucose + beta-D-glucopyranose 1-phosphate
-
-
-
r
3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate
-
727705
Lachnoclostridium phytofermentans DSM 18823
D-glucose + beta-D-glucopyranose 1-phosphate
-
-
-
r
D-galactose + beta-D-glucopyranose 1-phosphate
21% activity compared to D-glucose as acceptor
727705
Lachnoclostridium phytofermentans
3-O-alpha-D-glucopyranosyl-D-galactopyranose + phosphate
-
-
-
r
D-galactose + beta-D-glucopyranose 1-phosphate
21% activity compared to D-glucose as acceptor
727705
Lachnoclostridium phytofermentans DSM 18823
3-O-alpha-D-glucopyranosyl-D-galactopyranose + phosphate
-
-
-
r
D-glucose + beta-D-glucopyranose 1-phosphate
-
727705
Lachnoclostridium phytofermentans
3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate
-
-
-
r
D-glucose + beta-D-glucopyranose 1-phosphate
-
727705
Lachnoclostridium phytofermentans DSM 18823
3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate
-
-
-
r
D-xylose + beta-D-glucopyranose 1-phosphate
38% activity compared to D-glucose as acceptor
727705
Lachnoclostridium phytofermentans
3-O-alpha-D-glucopyranosyl-D-xylopyranose + phosphate
-
-
-
r
methyl alpha-D-glucopyranoside + beta-D-glucopyranose 1-phosphate
2.1% activity compared to D-glucose as acceptor
727705
Lachnoclostridium phytofermentans
methyl 3-O-alpha-D-glucopyranosyl-D-glucopyranoside + phosphate
-
-
-
r
additional information
with beta-D-glucopyranose 1-phosphate as donor the following sugars cannot act as acceptors: D-mannose, D-allose, D-fructose, D-lyxose, D-arabinose, L-arabinose, D-glucosamine, N-acetyl-D-glucosamine, N-acetyl-D-galactosamine, L-fucose, L-rhamnose, D-glucal, 2-deoxy-D-glucose, alpha-D-glucose 1-phosphate, alpha-D-galactose 1-phosphate, D-glucose 6-phosphate, methyl beta-D-glucoside, 3-O-methyl-D-glucose, trehalose, kojibiose, nigerose, maltose, isomaltose, sophorose, laminaribiose, cellobiose, gentiobiose, xylobiose, melibiose, lactose, sucrose
727705
Lachnoclostridium phytofermentans
?
-
-
-
-
additional information
with beta-D-glucopyranose 1-phosphate as donor the following sugars cannot act as acceptors: D-mannose, D-allose, D-fructose, D-lyxose, D-arabinose, L-arabinose, D-glucosamine, N-acetyl-D-glucosamine, N-acetyl-D-galactosamine, L-fucose, L-rhamnose, D-glucal, 2-deoxy-D-glucose, alpha-D-glucose 1-phosphate, alpha-D-galactose 1-phosphate, D-glucose 6-phosphate, methyl beta-D-glucoside, 3-O-methyl-D-glucose, trehalose, kojibiose, nigerose, maltose, isomaltose, sophorose, laminaribiose, cellobiose, gentiobiose, xylobiose, melibiose, lactose, sucrose
727705
Lachnoclostridium phytofermentans DSM 18823
?
-
-
-
-
Synonyms
Synonyms
Commentary
Organism
Cphy_3313
locus name
Lachnoclostridium phytofermentans
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
40
-
stable up to 40C
Lachnoclostridium phytofermentans
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
8.3
-
methyl alpha-D-glucopyranoside
pH 7.0, 30C, reverse phosphorolysis, donor beta-D-glucopyranose 1-phosphate
Lachnoclostridium phytofermentans
25
-
D-galactose
pH 7.0, 30C, reverse phosphorolysis, donor beta-D-glucopyranose 1-phosphate
Lachnoclostridium phytofermentans
30
-
1,5-anhydro-D-glucitol
pH 7.0, 30C, reverse phosphorolysis, donor beta-D-glucopyranose 1-phosphate
Lachnoclostridium phytofermentans
31
-
3-O-alpha-D-glucopyranosyl-D-glucopyranose
pH 7.0, 30C
Lachnoclostridium phytofermentans
40
-
D-xylose
pH 7.0, 30C, reverse phosphorolysis, donor beta-D-glucopyranose 1-phosphate
Lachnoclostridium phytofermentans
52
-
D-glucose
pH 7.0, 30C, reverse phosphorolysis, donor beta-D-glucopyranose 1-phosphate
Lachnoclostridium phytofermentans
58
-
beta-D-glucopyranose 1-phosphate
pH 7.0, 30C, reverse phosphorolysis, acceptor D-glucose
Lachnoclostridium phytofermentans
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
-
Lachnoclostridium phytofermentans
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
5.5
9
-
Lachnoclostridium phytofermentans
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BL21
Lachnoclostridium phytofermentans
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.49
-
phosphate
pH 7.0, 30C
Lachnoclostridium phytofermentans
1
7
1,5-anhydro-D-glucitol
pH 7.0, 30C, reverse phosphorolysis, donor beta-D-glucopyranose 1-phosphate
Lachnoclostridium phytofermentans
1
7
D-xylose
pH 7.0, 30C, reverse phosphorolysis, donor beta-D-glucopyranose 1-phosphate
Lachnoclostridium phytofermentans
1.2
-
beta-D-glucopyranose 1-phosphate
pH 7.0, 30C, reverse phosphorolysis, acceptor D-glucose
Lachnoclostridium phytofermentans
2.1
-
D-glucose
pH 7.0, 30C, reverse phosphorolysis, donor beta-D-glucopyranose 1-phosphate
Lachnoclostridium phytofermentans
20
-
D-galactose
pH 7.0, 30C, reverse phosphorolysis, donor beta-D-glucopyranose 1-phosphate
Lachnoclostridium phytofermentans
79
-
methyl alpha-D-glucopyranoside
pH 7.0, 30C, reverse phosphorolysis, donor beta-D-glucopyranose 1-phosphate
Lachnoclostridium phytofermentans
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
-
Lachnoclostridium phytofermentans
5829
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate
Lachnoclostridium phytofermentans
-
D-glucose + beta-D-glucopyranose 1-phosphate
-
-
r
3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate
Lachnoclostridium phytofermentans DSM 18823
-
D-glucose + beta-D-glucopyranose 1-phosphate
-
-
r
Purification (Commentary) (protein specific)
Commentary
Organism
-
Lachnoclostridium phytofermentans
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1,5-anhydro-D-glucitol + beta-D-glucopyranose 1-phosphate
26% activity compared to D-glucose as acceptor
727705
Lachnoclostridium phytofermentans
3-O-alpha-D-glucopyranosyl-1-5-anhydro-D-glucitol + phosphate
-
-
-
r
1,5-anhydro-D-glucitol + beta-D-glucopyranose 1-phosphate
26% activity compared to D-glucose as acceptor
727705
Lachnoclostridium phytofermentans DSM 18823
3-O-alpha-D-glucopyranosyl-1-5-anhydro-D-glucitol + phosphate
-
-
-
r
3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate
-
727705
Lachnoclostridium phytofermentans
D-glucose + beta-D-glucopyranose 1-phosphate
-
-
-
r
3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate
-
727705
Lachnoclostridium phytofermentans DSM 18823
D-glucose + beta-D-glucopyranose 1-phosphate
-
-
-
r
D-galactose + beta-D-glucopyranose 1-phosphate
21% activity compared to D-glucose as acceptor
727705
Lachnoclostridium phytofermentans
3-O-alpha-D-glucopyranosyl-D-galactopyranose + phosphate
-
-
-
r
D-galactose + beta-D-glucopyranose 1-phosphate
21% activity compared to D-glucose as acceptor
727705
Lachnoclostridium phytofermentans DSM 18823
3-O-alpha-D-glucopyranosyl-D-galactopyranose + phosphate
-
-
-
r
D-glucose + beta-D-glucopyranose 1-phosphate
-
727705
Lachnoclostridium phytofermentans
3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate
-
-
-
r
D-glucose + beta-D-glucopyranose 1-phosphate
-
727705
Lachnoclostridium phytofermentans DSM 18823
3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate
-
-
-
r
D-xylose + beta-D-glucopyranose 1-phosphate
38% activity compared to D-glucose as acceptor
727705
Lachnoclostridium phytofermentans
3-O-alpha-D-glucopyranosyl-D-xylopyranose + phosphate
-
-
-
r
methyl alpha-D-glucopyranoside + beta-D-glucopyranose 1-phosphate
2.1% activity compared to D-glucose as acceptor
727705
Lachnoclostridium phytofermentans
methyl 3-O-alpha-D-glucopyranosyl-D-glucopyranoside + phosphate
-
-
-
r
additional information
with beta-D-glucopyranose 1-phosphate as donor the following sugars cannot act as acceptors: D-mannose, D-allose, D-fructose, D-lyxose, D-arabinose, L-arabinose, D-glucosamine, N-acetyl-D-glucosamine, N-acetyl-D-galactosamine, L-fucose, L-rhamnose, D-glucal, 2-deoxy-D-glucose, alpha-D-glucose 1-phosphate, alpha-D-galactose 1-phosphate, D-glucose 6-phosphate, methyl beta-D-glucoside, 3-O-methyl-D-glucose, trehalose, kojibiose, nigerose, maltose, isomaltose, sophorose, laminaribiose, cellobiose, gentiobiose, xylobiose, melibiose, lactose, sucrose
727705
Lachnoclostridium phytofermentans
?
-
-
-
-
additional information
with beta-D-glucopyranose 1-phosphate as donor the following sugars cannot act as acceptors: D-mannose, D-allose, D-fructose, D-lyxose, D-arabinose, L-arabinose, D-glucosamine, N-acetyl-D-glucosamine, N-acetyl-D-galactosamine, L-fucose, L-rhamnose, D-glucal, 2-deoxy-D-glucose, alpha-D-glucose 1-phosphate, alpha-D-galactose 1-phosphate, D-glucose 6-phosphate, methyl beta-D-glucoside, 3-O-methyl-D-glucose, trehalose, kojibiose, nigerose, maltose, isomaltose, sophorose, laminaribiose, cellobiose, gentiobiose, xylobiose, melibiose, lactose, sucrose
727705
Lachnoclostridium phytofermentans DSM 18823
?
-
-
-
-
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
40
-
stable up to 40C
Lachnoclostridium phytofermentans
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
8.3
-
methyl alpha-D-glucopyranoside
pH 7.0, 30C, reverse phosphorolysis, donor beta-D-glucopyranose 1-phosphate
Lachnoclostridium phytofermentans
25
-
D-galactose
pH 7.0, 30C, reverse phosphorolysis, donor beta-D-glucopyranose 1-phosphate
Lachnoclostridium phytofermentans
30
-
1,5-anhydro-D-glucitol
pH 7.0, 30C, reverse phosphorolysis, donor beta-D-glucopyranose 1-phosphate
Lachnoclostridium phytofermentans
31
-
3-O-alpha-D-glucopyranosyl-D-glucopyranose
pH 7.0, 30C
Lachnoclostridium phytofermentans
40
-
D-xylose
pH 7.0, 30C, reverse phosphorolysis, donor beta-D-glucopyranose 1-phosphate
Lachnoclostridium phytofermentans
52
-
D-glucose
pH 7.0, 30C, reverse phosphorolysis, donor beta-D-glucopyranose 1-phosphate
Lachnoclostridium phytofermentans
58
-
beta-D-glucopyranose 1-phosphate
pH 7.0, 30C, reverse phosphorolysis, acceptor D-glucose
Lachnoclostridium phytofermentans
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
-
Lachnoclostridium phytofermentans
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
5.5
9
-
Lachnoclostridium phytofermentans
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.11
-
methyl alpha-D-glucopyranoside
pH 7.0, 30C, reverse phosphorolysis, donor beta-D-glucopyranose 1-phosphate
Lachnoclostridium phytofermentans
1.3
-
D-galactose
pH 7.0, 30C, reverse phosphorolysis, donor beta-D-glucopyranose 1-phosphate
Lachnoclostridium phytofermentans
1.8
-
1,5-anhydro-D-glucitol
pH 7.0, 30C, reverse phosphorolysis, donor beta-D-glucopyranose 1-phosphate
Lachnoclostridium phytofermentans
2.4
-
D-xylose
pH 7.0, 30C, reverse phosphorolysis, donor beta-D-glucopyranose 1-phosphate
Lachnoclostridium phytofermentans
24
-
D-glucose
pH 7.0, 30C, reverse phosphorolysis, donor beta-D-glucopyranose 1-phosphate
Lachnoclostridium phytofermentans
49
-
beta-D-glucopyranose 1-phosphate
pH 7.0, 30C, reverse phosphorolysis, acceptor D-glucose
Lachnoclostridium phytofermentans
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.11
-
methyl alpha-D-glucopyranoside
pH 7.0, 30C, reverse phosphorolysis, donor beta-D-glucopyranose 1-phosphate
Lachnoclostridium phytofermentans
1.3
-
D-galactose
pH 7.0, 30C, reverse phosphorolysis, donor beta-D-glucopyranose 1-phosphate
Lachnoclostridium phytofermentans
1.8
-
1,5-anhydro-D-glucitol
pH 7.0, 30C, reverse phosphorolysis, donor beta-D-glucopyranose 1-phosphate
Lachnoclostridium phytofermentans
2.4
-
D-xylose
pH 7.0, 30C, reverse phosphorolysis, donor beta-D-glucopyranose 1-phosphate
Lachnoclostridium phytofermentans
24
-
D-glucose
pH 7.0, 30C, reverse phosphorolysis, donor beta-D-glucopyranose 1-phosphate
Lachnoclostridium phytofermentans
49
-
beta-D-glucopyranose 1-phosphate
pH 7.0, 30C, reverse phosphorolysis, acceptor D-glucose
Lachnoclostridium phytofermentans
Other publictions for EC 2.4.1.279
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
727705
Nihira
-
Characterization of two phosph ...
Lachnoclostridium phytofermentans, Lachnoclostridium phytofermentans DSM 18823
J. Appl. Glycosci.
61
59-66
2014
-
-
1
-
-
-
-
7
1
-
-
2
-
2
-
-
1
-
-
-
-
-
12
-
1
-
-
1
7
1
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
7
1
-
-
2
-
-
-
1
-
-
-
-
12
-
-
-
1
7
1
-
1
-
-
-
-
-
6
6
721375
Nihira
Discovery of nigerose phosphor ...
Lachnoclostridium phytofermentans, Lachnoclostridium phytofermentans ATCC 700394
Appl. Microbiol. Biotechnol.
93
1513-1522
2012
-
-
1
-
-
-
7
6
1
1
1
2
-
4
-
-
-
1
-
-
6
-
14
-
1
1
-
-
5
1
-
1
-
1
-
-
-
-
1
-
-
-
-
-
7
1
6
1
1
1
2
-
-
-
-
-
-
6
-
14
-
1
-
-
5
1
-
1
-
-
-
-
-
4
4