Literature summary for 2.4.1.279 extracted from

Nihira, T.; Nishimoto, M.; Nakai, H.; Ohtsubo, K.; Kitaoka, M.
Characterization of two phosphorylases for alpha-1,3-oligoglucans from Clostridium phytofermentans (2014), J. Appl. Glycosci., 61, 59-66.

No PubMed abstract available

Search for more literature for 2.4.1.279

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21	Lachnoclostridium phytofermentans

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.49	-	phosphate	pH 7.0, 30°C	Lachnoclostridium phytofermentans
1	7	D-xylose	pH 7.0, 30°C, reverse phosphorolysis, donor beta-D-glucopyranose 1-phosphate	Lachnoclostridium phytofermentans
1	7	1,5-anhydro-D-glucitol	pH 7.0, 30°C, reverse phosphorolysis, donor beta-D-glucopyranose 1-phosphate	Lachnoclostridium phytofermentans
1.2	-	beta-D-glucopyranose 1-phosphate	pH 7.0, 30°C, reverse phosphorolysis, acceptor D-glucose	Lachnoclostridium phytofermentans
2.1	-	D-glucose	pH 7.0, 30°C, reverse phosphorolysis, donor beta-D-glucopyranose 1-phosphate	Lachnoclostridium phytofermentans
20	-	D-galactose	pH 7.0, 30°C, reverse phosphorolysis, donor beta-D-glucopyranose 1-phosphate	Lachnoclostridium phytofermentans
79	-	methyl alpha-D-glucopyranoside	pH 7.0, 30°C, reverse phosphorolysis, donor beta-D-glucopyranose 1-phosphate	Lachnoclostridium phytofermentans

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytosol	-	Lachnoclostridium phytofermentans	5829	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate	Lachnoclostridium phytofermentans	-	D-glucose + beta-D-glucopyranose 1-phosphate	-	r
3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate	Lachnoclostridium phytofermentans DSM 18823	-	D-glucose + beta-D-glucopyranose 1-phosphate	-	r

Organism

Organism	UniProt	Comment	Textmining
Lachnoclostridium phytofermentans	A9KSL5	-	-
Lachnoclostridium phytofermentans DSM 18823	A9KSL5	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Lachnoclostridium phytofermentans

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1,5-anhydro-D-glucitol + beta-D-glucopyranose 1-phosphate	26% activity compared to D-glucose as acceptor	Lachnoclostridium phytofermentans	3-O-alpha-D-glucopyranosyl-1-5-anhydro-D-glucitol + phosphate	-	r
1,5-anhydro-D-glucitol + beta-D-glucopyranose 1-phosphate	26% activity compared to D-glucose as acceptor	Lachnoclostridium phytofermentans DSM 18823	3-O-alpha-D-glucopyranosyl-1-5-anhydro-D-glucitol + phosphate	-	r
3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate	-	Lachnoclostridium phytofermentans	D-glucose + beta-D-glucopyranose 1-phosphate	-	r
3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate	-	Lachnoclostridium phytofermentans DSM 18823	D-glucose + beta-D-glucopyranose 1-phosphate	-	r
D-galactose + beta-D-glucopyranose 1-phosphate	21% activity compared to D-glucose as acceptor	Lachnoclostridium phytofermentans	3-O-alpha-D-glucopyranosyl-D-galactopyranose + phosphate	-	r
D-galactose + beta-D-glucopyranose 1-phosphate	21% activity compared to D-glucose as acceptor	Lachnoclostridium phytofermentans DSM 18823	3-O-alpha-D-glucopyranosyl-D-galactopyranose + phosphate	-	r
D-glucose + beta-D-glucopyranose 1-phosphate	-	Lachnoclostridium phytofermentans	3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate	-	r
D-glucose + beta-D-glucopyranose 1-phosphate	-	Lachnoclostridium phytofermentans DSM 18823	3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate	-	r
D-xylose + beta-D-glucopyranose 1-phosphate	38% activity compared to D-glucose as acceptor	Lachnoclostridium phytofermentans	3-O-alpha-D-glucopyranosyl-D-xylopyranose + phosphate	-	r
methyl alpha-D-glucopyranoside + beta-D-glucopyranose 1-phosphate	2.1% activity compared to D-glucose as acceptor	Lachnoclostridium phytofermentans	methyl 3-O-alpha-D-glucopyranosyl-D-glucopyranoside + phosphate	-	r
additional information	with beta-D-glucopyranose 1-phosphate as donor the following sugars cannot act as acceptors: D-mannose, D-allose, D-fructose, D-lyxose, D-arabinose, L-arabinose, D-glucosamine, N-acetyl-D-glucosamine, N-acetyl-D-galactosamine, L-fucose, L-rhamnose, D-glucal, 2-deoxy-D-glucose, alpha-D-glucose 1-phosphate, alpha-D-galactose 1-phosphate, D-glucose 6-phosphate, methyl beta-D-glucoside, 3-O-methyl-D-glucose, trehalose, kojibiose, nigerose, maltose, isomaltose, sophorose, laminaribiose, cellobiose, gentiobiose, xylobiose, melibiose, lactose, sucrose	Lachnoclostridium phytofermentans	?	-	?
additional information	with beta-D-glucopyranose 1-phosphate as donor the following sugars cannot act as acceptors: D-mannose, D-allose, D-fructose, D-lyxose, D-arabinose, L-arabinose, D-glucosamine, N-acetyl-D-glucosamine, N-acetyl-D-galactosamine, L-fucose, L-rhamnose, D-glucal, 2-deoxy-D-glucose, alpha-D-glucose 1-phosphate, alpha-D-galactose 1-phosphate, D-glucose 6-phosphate, methyl beta-D-glucoside, 3-O-methyl-D-glucose, trehalose, kojibiose, nigerose, maltose, isomaltose, sophorose, laminaribiose, cellobiose, gentiobiose, xylobiose, melibiose, lactose, sucrose	Lachnoclostridium phytofermentans DSM 18823	?	-	?

Synonyms

Synonyms	Comment	Organism
Cphy_3313	locus name	Lachnoclostridium phytofermentans

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
40	-	stable up to 40°C	Lachnoclostridium phytofermentans

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
8.3	-	methyl alpha-D-glucopyranoside	pH 7.0, 30°C, reverse phosphorolysis, donor beta-D-glucopyranose 1-phosphate	Lachnoclostridium phytofermentans
25	-	D-galactose	pH 7.0, 30°C, reverse phosphorolysis, donor beta-D-glucopyranose 1-phosphate	Lachnoclostridium phytofermentans
30	-	1,5-anhydro-D-glucitol	pH 7.0, 30°C, reverse phosphorolysis, donor beta-D-glucopyranose 1-phosphate	Lachnoclostridium phytofermentans
31	-	3-O-alpha-D-glucopyranosyl-D-glucopyranose	pH 7.0, 30°C	Lachnoclostridium phytofermentans
40	-	D-xylose	pH 7.0, 30°C, reverse phosphorolysis, donor beta-D-glucopyranose 1-phosphate	Lachnoclostridium phytofermentans
52	-	D-glucose	pH 7.0, 30°C, reverse phosphorolysis, donor beta-D-glucopyranose 1-phosphate	Lachnoclostridium phytofermentans
58	-	beta-D-glucopyranose 1-phosphate	pH 7.0, 30°C, reverse phosphorolysis, acceptor D-glucose	Lachnoclostridium phytofermentans

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	-	Lachnoclostridium phytofermentans

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
5.5	9	-	Lachnoclostridium phytofermentans

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
0.11	-	methyl alpha-D-glucopyranoside	pH 7.0, 30°C, reverse phosphorolysis, donor beta-D-glucopyranose 1-phosphate	Lachnoclostridium phytofermentans
1.3	-	D-galactose	pH 7.0, 30°C, reverse phosphorolysis, donor beta-D-glucopyranose 1-phosphate	Lachnoclostridium phytofermentans
1.8	-	1,5-anhydro-D-glucitol	pH 7.0, 30°C, reverse phosphorolysis, donor beta-D-glucopyranose 1-phosphate	Lachnoclostridium phytofermentans
2.4	-	D-xylose	pH 7.0, 30°C, reverse phosphorolysis, donor beta-D-glucopyranose 1-phosphate	Lachnoclostridium phytofermentans
24	-	D-glucose	pH 7.0, 30°C, reverse phosphorolysis, donor beta-D-glucopyranose 1-phosphate	Lachnoclostridium phytofermentans
49	-	beta-D-glucopyranose 1-phosphate	pH 7.0, 30°C, reverse phosphorolysis, acceptor D-glucose	Lachnoclostridium phytofermentans

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Release 2023.1 (January 2023)