BRENDA - Enzyme Database
show all sequences of 2.4.1.279

Discovery of nigerose phosphorylase from Clostridium phytofermentans

Nihira, T.; Nakai, H.; Chiku, K.; Kitaoka, M.; Appl. Microbiol. Biotechnol. 93, 1513-1522 (2012)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expression in Escherichia coli
Lachnoclostridium phytofermentans
Inhibitors
Inhibitors
Commentary
Organism
Structure
3-O-alpha-D-glucopyranosyl-D-glucopyranose
-
Lachnoclostridium phytofermentans
Co2+
1 mM, 80% inhibition
Lachnoclostridium phytofermentans
Cu2+
1 mM, 98.7% inhibition
Lachnoclostridium phytofermentans
Fe2+
1 mM, 68% inhibition
Lachnoclostridium phytofermentans
additional information
no inhibition by EDTA
Lachnoclostridium phytofermentans
Ni2+
1 mM, 95.4% inhibition
Lachnoclostridium phytofermentans
Zn2+
1 mM, 85% inhibition
Lachnoclostridium phytofermentans
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.2
-
phosphate
pH 7.0, 30°C, phosphorolysis reaction, cosubstrate 3-O-alpha-D-glucopyranosyl-D-glucopyranose
Lachnoclostridium phytofermentans
1.7
-
3-O-alpha-D-glucopyranosyl-D-glucopyranose
pH 7.0, 30°C, phosphorolyis reaction
Lachnoclostridium phytofermentans
3.3
-
D-glucose
pH 7.0, 30°C, reverse phosphorolysis reaction, cosubstrate beta-D-glucose 1-phosphate
Lachnoclostridium phytofermentans
79
-
1,5-anhydro-D-glucitol
pH 7.0, 30°C, reverse phosphorolysis reaction, cosubstrate beta-D-glucose 1-phosphate
Lachnoclostridium phytofermentans
86
-
D-xylose
pH 7.0, 30°C, reverse phosphorolysis reaction, cosubstrate beta-D-glucose 1-phosphate
Lachnoclostridium phytofermentans
104
-
D-galactose
pH 7.0, 30°C, reverse phosphorolysis reaction, cosubstrate beta-D-glucose 1-phosphate
Lachnoclostridium phytofermentans
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
-
Lachnoclostridium phytofermentans
5829
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
additional information
no divalent metal ions required
Lachnoclostridium phytofermentans
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
87170
-
calculated from amino acid sequence
Lachnoclostridium phytofermentans
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate
Lachnoclostridium phytofermentans
Nigerose may be liberated from nigeran outside the cell, transported into the cell by a sugar transporter and phosphorylyzed into beta-D-glucose 1-phosphate. This is converted into D-glucose 6-phosphate and can enter the glycolytic pathway.
D-glucose + beta-D-glucose 1-phosphate
-
-
r
3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate
Lachnoclostridium phytofermentans ATCC 700394
Nigerose may be liberated from nigeran outside the cell, transported into the cell by a sugar transporter and phosphorylyzed into beta-D-glucose 1-phosphate. This is converted into D-glucose 6-phosphate and can enter the glycolytic pathway.
D-glucose + beta-D-glucose 1-phosphate
-
-
r
Organism
Organism
UniProt
Commentary
Textmining
Lachnoclostridium phytofermentans
A9KT32
-
-
Lachnoclostridium phytofermentans ATCC 700394
A9KT32
-
-
Reaction
Reaction
Commentary
Organism
Reaction ID
3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate = D-glucose + beta-D-glucose 1-phosphate
sequential bi-bi mechanism
Lachnoclostridium phytofermentans
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.17
-
pH 7.0, 30°C, phosphorolysis reaction, substrate 2-O-alpha-D-glucopyranosyl-D-glucopyranose; pH 7.0, 30°C, reverse phosphorolysis reaction, substrates beta-D-glucose 1-phosphate + methyl alpha-D-glucoside
Lachnoclostridium phytofermentans
4.6
-
pH 7.0, 30°C, reverse phosphorolysis reaction, substrates beta-D-glucose 1-phosphate + D-galactose
Lachnoclostridium phytofermentans
8.1
-
pH 7.0, 30°C, reverse phosphorolysis reaction, substrates beta-D-glucose 1-phosphate + 1,5-anhydro-D-glucitol
Lachnoclostridium phytofermentans
9.3
-
pH 7.0, 30°C, reverse phosphorolysis reaction, substrates beta-D-glucose 1-phosphate + D-xylose
Lachnoclostridium phytofermentans
32
-
pH 7.0, 30°C, phosphorolysis reaction, substrate 3-O-alpha-D-glucopyranosyl-D-glucopyranose
Lachnoclostridium phytofermentans
54
-
pH 7.0, 30°C, reverse phosphorolysis reaction, substrates beta-D-glucose 1-phosphate + D-glucose
Lachnoclostridium phytofermentans
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
2-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate
very weak phosphorolysis reaction with kojibiose, 0.5% of the activity with nigerose
721375
Lachnoclostridium phytofermentans
D-glucose + beta-D-glucose 1-phosphate
-
-
-
r
2-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate
very weak phosphorolysis reaction with kojibiose, 0.5% of the activity with nigerose
721375
Lachnoclostridium phytofermentans ATCC 700394
D-glucose + beta-D-glucose 1-phosphate
-
-
-
r
3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate
Nigerose may be liberated from nigeran outside the cell, transported into the cell by a sugar transporter and phosphorylyzed into beta-D-glucose 1-phosphate. This is converted into D-glucose 6-phosphate and can enter the glycolytic pathway.
721375
Lachnoclostridium phytofermentans
D-glucose + beta-D-glucose 1-phosphate
-
-
-
r
3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate
phosphorolysis reaction with nigerose
721375
Lachnoclostridium phytofermentans
D-glucose + beta-D-glucose 1-phosphate
-
-
-
r
3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate
Nigerose may be liberated from nigeran outside the cell, transported into the cell by a sugar transporter and phosphorylyzed into beta-D-glucose 1-phosphate. This is converted into D-glucose 6-phosphate and can enter the glycolytic pathway.
721375
Lachnoclostridium phytofermentans ATCC 700394
D-glucose + beta-D-glucose 1-phosphate
-
-
-
r
3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate
phosphorolysis reaction with nigerose
721375
Lachnoclostridium phytofermentans ATCC 700394
D-glucose + beta-D-glucose 1-phosphate
-
-
-
r
beta-D-glucose 1-phosphate + 1,5-anhydro-D-glucitol
reverse phosphorolysis reaction 15% of the activity with 3-O-alpha-D-glucopyranosyl-D-glucopyranose
721375
Lachnoclostridium phytofermentans
3-O-alpha-D-glucopyranosyl-1,5-anhydro-D-glucitol + phosphate
-
-
-
?
beta-D-glucose 1-phosphate + 1,5-anhydro-D-glucitol
reverse phosphorolysis reaction 15% of the activity with 3-O-alpha-D-glucopyranosyl-D-glucopyranose
721375
Lachnoclostridium phytofermentans ATCC 700394
3-O-alpha-D-glucopyranosyl-1,5-anhydro-D-glucitol + phosphate
-
-
-
?
beta-D-glucose 1-phosphate + D-galactose
reverse phosphorolysis reaction 8.5% of the activity with 3-O-alpha-D-glucopyranosyl-D-glucopyranose, no significant preference for specific anomer of the acceptor
721375
Lachnoclostridium phytofermentans
3-O-alpha-D-glucopyranosyl-D-galactopyranose + phosphate
-
-
-
?
beta-D-glucose 1-phosphate + D-glucose
reverse phosphorolysis reaction, no significant preference for specific anomer of the acceptor
721375
Lachnoclostridium phytofermentans
3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate
-
-
-
r
beta-D-glucose 1-phosphate + D-xylose
reverse phosphorolysis reaction 17% of the activity with 3-O-alpha-D-glucopyranosyl-D-glucopyranose
721375
Lachnoclostridium phytofermentans
3-O-alpha-D-glucopyranosyl-D-xylopyranose + 2-O-alpha-D-glucopyranosyl-D-xylopyranose + phosphate
products are formed in the ratio 63:37%
-
-
?
beta-D-glucose 1-phosphate + methyl alpha-D-glucoside
reverse phosphorolysis reaction 17% of the activity with 3-O-alpha-D-glucopyranosyl-D-glucopyranose
721375
Lachnoclostridium phytofermentans
methyl-3-O-alpha-D-glucopyranosyl-alpha-D-glucopyranoside + methyl-2-O-alpha-D-glucopyranosyl-alpha-D-glucopyranoside + phosphate
products are formed in the ratio 71:29%
-
-
?
additional information
in the reverse phosphorolyis reaction the enzyme produces only disaccharides. The enzyme does not use any disaccharides as acceptors in the reverse phosphorolysis reaction. It does not use any D-glucose derivatives at the C2 position such as D-mannose, 2-deoxy-D-glucose, D-glucosamine, N-acetyl-D-glucosamine
721375
Lachnoclostridium phytofermentans
?
-
-
-
-
additional information
in the reverse phosphorolyis reaction the enzyme produces only disaccharides. The enzyme does not use any disaccharides as acceptors in the reverse phosphorolysis reaction. It does not use any D-glucose derivatives at the C2 position such as D-mannose, 2-deoxy-D-glucose, D-glucosamine, N-acetyl-D-glucosamine
721375
Lachnoclostridium phytofermentans ATCC 700394
?
-
-
-
-
Synonyms
Synonyms
Commentary
Organism
cphy1874
-
Lachnoclostridium phytofermentans
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
40
-
-
Lachnoclostridium phytofermentans
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
67
-
phosphate
pH 7.0, 30°C, cosubstrate 3-O-alpha-D-glucopyranosyl-D-glucopyranose
Lachnoclostridium phytofermentans
69
-
D-galactose
pH 7.0, 30°C, reverse phosphorolysis reaction, cosubstrate beta-D-glucose 1-phosphate
Lachnoclostridium phytofermentans
110
-
D-glucose
pH 7.0, 30°C, reverse phosphorolysis reaction, cosubstrate beta-D-glucose 1-phosphate
Lachnoclostridium phytofermentans
118
-
D-xylose
pH 7.0, 30°C, reverse phosphorolysis reaction, cosubstrate beta-D-glucose 1-phosphate
Lachnoclostridium phytofermentans
120
-
1,5-anhydro-D-glucitol
pH 7.0, 30°C, reverse phosphorolysis reaction, cosubstrate beta-D-glucose 1-phosphate
Lachnoclostridium phytofermentans
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
-
Lachnoclostridium phytofermentans
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
5.5
9
-
Lachnoclostridium phytofermentans
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
6.4
-
3-O-alpha-D-glucopyranosyl-D-glucopyranose
pH 7.0, 30°C
Lachnoclostridium phytofermentans
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Lachnoclostridium phytofermentans
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
3-O-alpha-D-glucopyranosyl-D-glucopyranose
-
Lachnoclostridium phytofermentans
Co2+
1 mM, 80% inhibition
Lachnoclostridium phytofermentans
Cu2+
1 mM, 98.7% inhibition
Lachnoclostridium phytofermentans
Fe2+
1 mM, 68% inhibition
Lachnoclostridium phytofermentans
additional information
no inhibition by EDTA
Lachnoclostridium phytofermentans
Ni2+
1 mM, 95.4% inhibition
Lachnoclostridium phytofermentans
Zn2+
1 mM, 85% inhibition
Lachnoclostridium phytofermentans
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
6.4
-
3-O-alpha-D-glucopyranosyl-D-glucopyranose
pH 7.0, 30°C
Lachnoclostridium phytofermentans
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.2
-
phosphate
pH 7.0, 30°C, phosphorolysis reaction, cosubstrate 3-O-alpha-D-glucopyranosyl-D-glucopyranose
Lachnoclostridium phytofermentans
1.7
-
3-O-alpha-D-glucopyranosyl-D-glucopyranose
pH 7.0, 30°C, phosphorolyis reaction
Lachnoclostridium phytofermentans
3.3
-
D-glucose
pH 7.0, 30°C, reverse phosphorolysis reaction, cosubstrate beta-D-glucose 1-phosphate
Lachnoclostridium phytofermentans
79
-
1,5-anhydro-D-glucitol
pH 7.0, 30°C, reverse phosphorolysis reaction, cosubstrate beta-D-glucose 1-phosphate
Lachnoclostridium phytofermentans
86
-
D-xylose
pH 7.0, 30°C, reverse phosphorolysis reaction, cosubstrate beta-D-glucose 1-phosphate
Lachnoclostridium phytofermentans
104
-
D-galactose
pH 7.0, 30°C, reverse phosphorolysis reaction, cosubstrate beta-D-glucose 1-phosphate
Lachnoclostridium phytofermentans
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
-
Lachnoclostridium phytofermentans
5829
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
additional information
no divalent metal ions required
Lachnoclostridium phytofermentans
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
87170
-
calculated from amino acid sequence
Lachnoclostridium phytofermentans
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate
Lachnoclostridium phytofermentans
Nigerose may be liberated from nigeran outside the cell, transported into the cell by a sugar transporter and phosphorylyzed into beta-D-glucose 1-phosphate. This is converted into D-glucose 6-phosphate and can enter the glycolytic pathway.
D-glucose + beta-D-glucose 1-phosphate
-
-
r
3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate
Lachnoclostridium phytofermentans ATCC 700394
Nigerose may be liberated from nigeran outside the cell, transported into the cell by a sugar transporter and phosphorylyzed into beta-D-glucose 1-phosphate. This is converted into D-glucose 6-phosphate and can enter the glycolytic pathway.
D-glucose + beta-D-glucose 1-phosphate
-
-
r
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.17
-
pH 7.0, 30°C, phosphorolysis reaction, substrate 2-O-alpha-D-glucopyranosyl-D-glucopyranose; pH 7.0, 30°C, reverse phosphorolysis reaction, substrates beta-D-glucose 1-phosphate + methyl alpha-D-glucoside
Lachnoclostridium phytofermentans
4.6
-
pH 7.0, 30°C, reverse phosphorolysis reaction, substrates beta-D-glucose 1-phosphate + D-galactose
Lachnoclostridium phytofermentans
8.1
-
pH 7.0, 30°C, reverse phosphorolysis reaction, substrates beta-D-glucose 1-phosphate + 1,5-anhydro-D-glucitol
Lachnoclostridium phytofermentans
9.3
-
pH 7.0, 30°C, reverse phosphorolysis reaction, substrates beta-D-glucose 1-phosphate + D-xylose
Lachnoclostridium phytofermentans
32
-
pH 7.0, 30°C, phosphorolysis reaction, substrate 3-O-alpha-D-glucopyranosyl-D-glucopyranose
Lachnoclostridium phytofermentans
54
-
pH 7.0, 30°C, reverse phosphorolysis reaction, substrates beta-D-glucose 1-phosphate + D-glucose
Lachnoclostridium phytofermentans
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
2-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate
very weak phosphorolysis reaction with kojibiose, 0.5% of the activity with nigerose
721375
Lachnoclostridium phytofermentans
D-glucose + beta-D-glucose 1-phosphate
-
-
-
r
2-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate
very weak phosphorolysis reaction with kojibiose, 0.5% of the activity with nigerose
721375
Lachnoclostridium phytofermentans ATCC 700394
D-glucose + beta-D-glucose 1-phosphate
-
-
-
r
3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate
Nigerose may be liberated from nigeran outside the cell, transported into the cell by a sugar transporter and phosphorylyzed into beta-D-glucose 1-phosphate. This is converted into D-glucose 6-phosphate and can enter the glycolytic pathway.
721375
Lachnoclostridium phytofermentans
D-glucose + beta-D-glucose 1-phosphate
-
-
-
r
3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate
phosphorolysis reaction with nigerose
721375
Lachnoclostridium phytofermentans
D-glucose + beta-D-glucose 1-phosphate
-
-
-
r
3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate
Nigerose may be liberated from nigeran outside the cell, transported into the cell by a sugar transporter and phosphorylyzed into beta-D-glucose 1-phosphate. This is converted into D-glucose 6-phosphate and can enter the glycolytic pathway.
721375
Lachnoclostridium phytofermentans ATCC 700394
D-glucose + beta-D-glucose 1-phosphate
-
-
-
r
3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate
phosphorolysis reaction with nigerose
721375
Lachnoclostridium phytofermentans ATCC 700394
D-glucose + beta-D-glucose 1-phosphate
-
-
-
r
beta-D-glucose 1-phosphate + 1,5-anhydro-D-glucitol
reverse phosphorolysis reaction 15% of the activity with 3-O-alpha-D-glucopyranosyl-D-glucopyranose
721375
Lachnoclostridium phytofermentans
3-O-alpha-D-glucopyranosyl-1,5-anhydro-D-glucitol + phosphate
-
-
-
?
beta-D-glucose 1-phosphate + 1,5-anhydro-D-glucitol
reverse phosphorolysis reaction 15% of the activity with 3-O-alpha-D-glucopyranosyl-D-glucopyranose
721375
Lachnoclostridium phytofermentans ATCC 700394
3-O-alpha-D-glucopyranosyl-1,5-anhydro-D-glucitol + phosphate
-
-
-
?
beta-D-glucose 1-phosphate + D-galactose
reverse phosphorolysis reaction 8.5% of the activity with 3-O-alpha-D-glucopyranosyl-D-glucopyranose, no significant preference for specific anomer of the acceptor
721375
Lachnoclostridium phytofermentans
3-O-alpha-D-glucopyranosyl-D-galactopyranose + phosphate
-
-
-
?
beta-D-glucose 1-phosphate + D-glucose
reverse phosphorolysis reaction, no significant preference for specific anomer of the acceptor
721375
Lachnoclostridium phytofermentans
3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate
-
-
-
r
beta-D-glucose 1-phosphate + D-xylose
reverse phosphorolysis reaction 17% of the activity with 3-O-alpha-D-glucopyranosyl-D-glucopyranose
721375
Lachnoclostridium phytofermentans
3-O-alpha-D-glucopyranosyl-D-xylopyranose + 2-O-alpha-D-glucopyranosyl-D-xylopyranose + phosphate
products are formed in the ratio 63:37%
-
-
?
beta-D-glucose 1-phosphate + methyl alpha-D-glucoside
reverse phosphorolysis reaction 17% of the activity with 3-O-alpha-D-glucopyranosyl-D-glucopyranose
721375
Lachnoclostridium phytofermentans
methyl-3-O-alpha-D-glucopyranosyl-alpha-D-glucopyranoside + methyl-2-O-alpha-D-glucopyranosyl-alpha-D-glucopyranoside + phosphate
products are formed in the ratio 71:29%
-
-
?
additional information
in the reverse phosphorolyis reaction the enzyme produces only disaccharides. The enzyme does not use any disaccharides as acceptors in the reverse phosphorolysis reaction. It does not use any D-glucose derivatives at the C2 position such as D-mannose, 2-deoxy-D-glucose, D-glucosamine, N-acetyl-D-glucosamine
721375
Lachnoclostridium phytofermentans
?
-
-
-
-
additional information
in the reverse phosphorolyis reaction the enzyme produces only disaccharides. The enzyme does not use any disaccharides as acceptors in the reverse phosphorolysis reaction. It does not use any D-glucose derivatives at the C2 position such as D-mannose, 2-deoxy-D-glucose, D-glucosamine, N-acetyl-D-glucosamine
721375
Lachnoclostridium phytofermentans ATCC 700394
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
40
-
-
Lachnoclostridium phytofermentans
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
67
-
phosphate
pH 7.0, 30°C, cosubstrate 3-O-alpha-D-glucopyranosyl-D-glucopyranose
Lachnoclostridium phytofermentans
69
-
D-galactose
pH 7.0, 30°C, reverse phosphorolysis reaction, cosubstrate beta-D-glucose 1-phosphate
Lachnoclostridium phytofermentans
110
-
D-glucose
pH 7.0, 30°C, reverse phosphorolysis reaction, cosubstrate beta-D-glucose 1-phosphate
Lachnoclostridium phytofermentans
118
-
D-xylose
pH 7.0, 30°C, reverse phosphorolysis reaction, cosubstrate beta-D-glucose 1-phosphate
Lachnoclostridium phytofermentans
120
-
1,5-anhydro-D-glucitol
pH 7.0, 30°C, reverse phosphorolysis reaction, cosubstrate beta-D-glucose 1-phosphate
Lachnoclostridium phytofermentans
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
-
Lachnoclostridium phytofermentans
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
5.5
9
-
Lachnoclostridium phytofermentans
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.7
-
D-galactose
pH 7.0, 30°C, reverse phosphorolysis reaction, cosubstrate beta-D-glucose 1-phosphate
Lachnoclostridium phytofermentans
1.4
-
D-xylose
pH 7.0, 30°C, reverse phosphorolysis reaction, cosubstrate beta-D-glucose 1-phosphate
Lachnoclostridium phytofermentans
1.5
-
1,5-anhydro-D-glucitol
pH 7.0, 30°C, reverse phosphorolysis reaction, cosubstrate beta-D-glucose 1-phosphate
Lachnoclostridium phytofermentans
33
-
D-glucose
pH 7.0, 30°C, reverse phosphorolysis reaction, cosubstrate beta-D-glucose 1-phosphate
Lachnoclostridium phytofermentans
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.7
-
D-galactose
pH 7.0, 30°C, reverse phosphorolysis reaction, cosubstrate beta-D-glucose 1-phosphate
Lachnoclostridium phytofermentans
1.4
-
D-xylose
pH 7.0, 30°C, reverse phosphorolysis reaction, cosubstrate beta-D-glucose 1-phosphate
Lachnoclostridium phytofermentans
1.5
-
1,5-anhydro-D-glucitol
pH 7.0, 30°C, reverse phosphorolysis reaction, cosubstrate beta-D-glucose 1-phosphate
Lachnoclostridium phytofermentans
33
-
D-glucose
pH 7.0, 30°C, reverse phosphorolysis reaction, cosubstrate beta-D-glucose 1-phosphate
Lachnoclostridium phytofermentans
Other publictions for EC 2.4.1.279
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
727705
Nihira
-
Characterization of two phosph ...
Lachnoclostridium phytofermentans, Lachnoclostridium phytofermentans DSM 18823
J. Appl. Glycosci.
61
59-66
2014
-
-
1
-
-
-
-
7
1
-
-
2
-
2
-
-
1
-
-
-
-
-
12
-
1
-
-
1
7
1
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
7
1
-
-
2
-
-
-
1
-
-
-
-
12
-
-
-
1
7
1
-
1
-
-
-
-
-
6
6
721375
Nihira
Discovery of nigerose phosphor ...
Lachnoclostridium phytofermentans, Lachnoclostridium phytofermentans ATCC 700394
Appl. Microbiol. Biotechnol.
93
1513-1522
2012
-
-
1
-
-
-
7
6
1
1
1
2
-
4
-
-
-
1
-
-
6
-
14
-
1
1
-
-
5
1
-
1
-
1
-
-
-
-
1
-
-
-
-
-
7
1
6
1
1
1
2
-
-
-
-
-
-
6
-
14
-
1
-
-
5
1
-
1
-
-
-
-
-
4
4