BRENDA - Enzyme Database show
show all sequences of 2.4.1.276

Functional expression of zeaxanthin glucosyltransferase from Erwinia herbicola and a proposed uridine diphosphate binding site

Hundle, B.S.; OBrien, D.A.; Alberti, M.; Beyer, P.; Hearst, J.E.; Proc. Natl. Acad. Sci. USA 89, 9321-9325 (1992)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli
Pseudescherichia vulneris
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
weakly membrane-associated, without apparent membrane-spanning regions
Pseudescherichia vulneris
16020
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
45000
-
x * 45000, SDS-PAGE
Pseudescherichia vulneris
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudescherichia vulneris
Q01330
-
-
Reaction
Reaction
Commentary
Organism
2 UDP-glucose + zeaxanthin = 2 UDP + zeaxanthin bis(beta-D-glucoside)
binding of zeaxanthin is the rate-limiting step for the reaction sequence. The monoglucoside, as an intermediate, never reaches very high levels compared with zeaxanthin
Pseudescherichia vulneris
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
zeaxanthin + 2 UDP-D-glucose
-
716795
Pseudescherichia vulneris
zeaxanthin bis(beta-D-glucoside) + 2 UDP
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 45000, SDS-PAGE
Pseudescherichia vulneris
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
32
37
-
Pseudescherichia vulneris
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
7.5
-
Pseudescherichia vulneris
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Pseudescherichia vulneris
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
weakly membrane-associated, without apparent membrane-spanning regions
Pseudescherichia vulneris
16020
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
45000
-
x * 45000, SDS-PAGE
Pseudescherichia vulneris
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
zeaxanthin + 2 UDP-D-glucose
-
716795
Pseudescherichia vulneris
zeaxanthin bis(beta-D-glucoside) + 2 UDP
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 45000, SDS-PAGE
Pseudescherichia vulneris
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
32
37
-
Pseudescherichia vulneris
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
7.5
-
Pseudescherichia vulneris
Other publictions for EC 2.4.1.276
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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715871
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J. Microbiol. Biotechnol.
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4
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1
1
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1
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2
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1
1
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716795
Hundle
Functional expression of zeaxa ...
Pseudescherichia vulneris
Proc. Natl. Acad. Sci. USA
89
9321-9325
1992
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1
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1
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1
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3
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1
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1
1
1
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1
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1
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1
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1
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1
1
1
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1
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