BRENDA - Enzyme Database
show all sequences of 2.4.1.276

Production of caloxanthin 3'-beta-D-glucoside, zeaxanthin 3,3'-beta-D-diglucoside, and nostoxanthin in a recombinant Escherichia coli expressing system harboring seven carotenoid biosynthesis genes, including crtX and crtG

Osawa, A.; Harada, H.; Choi, S.; Misawa, N.; Shindo, K.; Phytochemistry 72, 711-716 (2011)

Data extracted from this reference:

Application
Application
Commentary
Organism
synthesis
production of rare beta-carotene-modified carotenoids possessing 2-O, 2-H or 2-glucosyl and/or 3-O, 3-H or 3-glucosyl functionalities in their beta-ionone rings using a recombinant Escherichia coli approach, involving expression of carotenoid biosynthesis genes crtE, crtB, crtI, crtY, crtZ, crtX and crtG. From the cells of the recombinant Escherichia coli, caloxanthin i.e. (beta,beta-carotene-2,3,2',3'-tetrol)-3'-beta-D-glucose, zeaxanthin i.e. (beta,beta-carotene-3,3'-diol) 3,3'-beta-D-diglucoside, and nostoxanthin i.e. (beta,beta-carotene-2,3,3'-triol) are isolated and identified. Caloxanthin 3'-beta-D-glucoside displays potent 1O2 quenching activity
Pantoea ananatis
Organism
Organism
UniProt
Commentary
Textmining
Pantoea ananatis
P21686
-
-
Synonyms
Synonyms
Commentary
Organism
crtX
-
Pantoea ananatis
Application (protein specific)
Application
Commentary
Organism
synthesis
production of rare beta-carotene-modified carotenoids possessing 2-O, 2-H or 2-glucosyl and/or 3-O, 3-H or 3-glucosyl functionalities in their beta-ionone rings using a recombinant Escherichia coli approach, involving expression of carotenoid biosynthesis genes crtE, crtB, crtI, crtY, crtZ, crtX and crtG. From the cells of the recombinant Escherichia coli, caloxanthin i.e. (beta,beta-carotene-2,3,2',3'-tetrol)-3'-beta-D-glucose, zeaxanthin i.e. (beta,beta-carotene-3,3'-diol) 3,3'-beta-D-diglucoside, and nostoxanthin i.e. (beta,beta-carotene-2,3,3'-triol) are isolated and identified. Caloxanthin 3'-beta-D-glucoside displays potent 1O2 quenching activity
Pantoea ananatis
Other publictions for EC 2.4.1.276
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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