BRENDA - Enzyme Database
show all sequences of 2.4.1.276

Production of lycopene by metabolically-engineered Escherichia coli

Sun, T.; Miao, L.; Li, Q.; Dai, G.; Lu, F.; Liu, T.; Zhang, X.; Ma, Y.; Biotechnol. Lett. 36, 1515-1522 (2014)

Data extracted from this reference:

Application
Application
Commentary
Organism
synthesis
Escherichia coli strain CAR001 carries the crtEXYIB operon of Enterobacter agglomerans to produce beta-carotene. Deletion of genes encoding zeaxanthin glucosyltransferase (crtX) and lycopene beta-cyclase (crtY) from the operon leads to production of 10.5 mg lycopene/l. Further optimization results in a strain producing 3.52 g lycopene/l in fed-batch fermentation
Pantoea agglomerans
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pantoea agglomerans
L0BH17
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-
Application (protein specific)
Application
Commentary
Organism
synthesis
Escherichia coli strain CAR001 carries the crtEXYIB operon of Enterobacter agglomerans to produce beta-carotene. Deletion of genes encoding zeaxanthin glucosyltransferase (crtX) and lycopene beta-cyclase (crtY) from the operon leads to production of 10.5 mg lycopene/l. Further optimization results in a strain producing 3.52 g lycopene/l in fed-batch fermentation
Pantoea agglomerans
Other publictions for EC 2.4.1.276
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
735830
Sun
Production of lycopene by meta ...
Pantoea agglomerans
Biotechnol. Lett.
36
1515-1522
2014
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735540
Choi
3-beta-Glucosyl-3'-beta-quinov ...
Pantoea ananatis
Appl. Microbiol. Biotechnol.
97
8479-8486
2013
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716468
Osawa
Production of caloxanthin 3'-b ...
Pantoea ananatis
Phytochemistry
72
711-716
2011
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715871
Seo
Cloning and characterization o ...
Paracoccus haeundaensis
J. Microbiol. Biotechnol.
19
1542-1546
2009
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1
1
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716795
Hundle
Functional expression of zeaxa ...
Pseudescherichia vulneris
Proc. Natl. Acad. Sci. USA
89
9321-9325
1992
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