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Literature summary for 2.4.1.275 extracted from

  • Schwientek, T.; Almeida, R.M., Levery, S.B.; Holmes, E.H.; Bennett, E.; Clausen, H.
    Cloning of a novel member of the UDP-galactose:beta-N-acetylglucosamine beta1,4-galactosyltransferase family, beta4Gal-T4, involved in glycosphingolipid biosynthesis (1998), J. Biol. Chem., 273, 29331-29340.
    View publication on PubMed
Search for more literature for 2.4.1.275

Activating Compound

Activating Compound Comment Organism Structure
alpha-lactalbumin the enzyme shows a marked increase in N-acetyllactosamine synthase activity in the presence of alpha-lactalbumin. Two-fold activation is achieved at 0.25 mg/ml and almost 8fold at 20 mg/ml Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expression in insect cells Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
additional information the enzyme is not inhibited at high concentrations of either benzyl-beta-D-N-acetyl-D-glucosamine or free N-acetylglucosamine Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-alpha-D-galactose + N-acetyl-beta-D-galactosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide Homo sapiens
-
 UDP + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-galactosaminyl-(1->3)-beta-D-galactosyl-(1->4)-D-glucosyl-(1<->1)-ceramide
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens O60513
-
-

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
 Homo sapiens
-
central nervous system
-
 Homo sapiens
-
colon
-
 Homo sapiens
-
heart
-
 Homo sapiens
-
lung
-
 Homo sapiens
-
muscle
-
 Homo sapiens
-
ovary
-
 Homo sapiens
-
placenta
-
 Homo sapiens
-
testis
-
 Homo sapiens
-
uterus
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information free glucose is not an acceptor for the enzyme. The enzyme does not transfer galactose to asialo-agalacto-fetuin, asialo-agalactotransferrin, or ovalbumin Homo sapiens ?
-
 ?
UDP-alpha-D-galactose + 1-O-benzyl 6-O-beta-D-GlcpNAC-beta-D-GlypNAc
-
 Homo sapiens UDP + 1-O-benzyl 4-O-beta-D-Galp-6-O-beta-D-GlcpNAC-beta-D-GlcpNAc
-
 ?
UDP-alpha-D-galactose + 1-O-benzyl beta-D-GlcpNAc no activity with alpha-D-GlcNAc-1-benzyl Homo sapiens UDP + 1-O-benzyl 4-O-beta-D-Galp-beta-D-GlcpNAc
-
 ?
UDP-alpha-D-galactose + 1-O-methyl 3-O-beta-D-GlcpNAc-beta-D-Galp
-
 Homo sapiens UDP + 1-O-methyl 4-O-beta-D-Galp-3-O-beta-D-GlcpNAc-beta-D-Galp
-
 ?
UDP-alpha-D-galactose + N-acetyl-beta-D-galactosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide
-
 Homo sapiens UDP + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-galactosaminyl-(1->3)-beta-D-galactosyl-(1->4)-D-glucosyl-(1<->1)-ceramide
-
 ?
UDP-alpha-D-galactose + N-acetyl-beta-D-galactosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide N-acetyl-beta-D-galactosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->)-ceramide i.e. lactotriaosylceramide. beta4Gal-T4 shows strict donor substrate specificity for UDP-Gal and did not utilize UDP-GalNAc or UDP-GlcNAc with the acceptor substrates tested Homo sapiens UDP + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-galactosaminyl-(1->3)-beta-D-galactosyl-(1->4)-D-glucosyl-(1<->1)-ceramide
-
 ?
UDP-galactose + 1-O-(1-methylumbellifer-7-yl) beta-D-GlcpNAc
-
 Homo sapiens UDP + 1-O-(1-methylumbellifer-7-yl) 4-O-beta-D-Galp-beta-D-GlcpNAc
-
 ?
UDP-galactose + 1-O-(4-nitrophenyl) beta-D-GlcpNAc
-
 Homo sapiens UDP + 1-O-(4-nitrophenyl) 4-O-beta-D-Galp-beta-D-GlcpNAc
-
 ?
UDP-galactose + 1-O-methyl 6-O-beta-D-GlcpNAC-alpha-D-Manp
-
 Homo sapiens UDP + 1-O-methyl 4-O-beta-D-Galp-6-O-beta-D-GlcpNAC-alpha-D-Manp
-
 ?
UDP-galactose + 1-S-(4-nitrophenyl) beta-D-1-thio-GlcpNAc
-
 Homo sapiens UDP + 1-S-(4-nitrophenyl) 4-O-beta-D-Galp-beta-D-1-thio-GlcpNAc
-
 ?
UDP-galactose + beta-D-GlcpNAc-(1->6)-D-GlcpNAc
-
 Homo sapiens UDP + beta-D-Galp-(1->4)-[beta-D-GlcpNAc-(1->6)]-D-GlcpNAc
-
 ?
UDP-galactose + N,N',N''-triacetylchitotriose
-
 Homo sapiens UDP + beta-D-Galp-(1->4)-beta-D-GlcpNAc-(1->4)-beta-D-GlcpNAc-(1->4)-D-GlcpNAc
-
 ?
UDP-galactose + N,N'-diacetylchitobiose
-
 Homo sapiens UDP + beta-D-Galp-(1->4)-beta-D-GlcpNAc-(1->4)-D-GlcpNAc
-
 ?

Synonyms

Synonyms Comment Organism
beta4Gal-T4
-
 Homo sapiens
UDP-galactose:beta-N-acetylglucosamine beta1,4-galactosyltransferase
-
 Homo sapiens
UDPGal:betaGlcNAc beta1,4Gal-transferase
-
 Homo sapiens