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Literature summary for 2.4.1.270 extracted from

  • Cunha, S.; dAvo, A.F.; Mingote, A.; Lamosa, P.; da Costa, M.S.; Costa, J.
    Mannosylglucosylglycerate biosynthesis in the deep-branching phylum Planctomycetes: characterization of the uncommon enzymes from Rhodopirellula baltica (2013), Sci. Rep., 3, 2378.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.1.270

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Rhodopirellula baltica

Inhibitors

Inhibitors Comment Organism Structure
EDTA 5 mM, 47% residual activity Rhodopirellula baltica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.28
-
 GDP-mannose pH 7.5, 40°C Rhodopirellula baltica
0.41
-
 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate pH 7.5, 25°C Rhodopirellula baltica

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ at 10 mM, high stimulation Rhodopirellula baltica
Co2+ at 1 mM, high stimulation Rhodopirellula baltica
Mn2+ maximum activtiy in presence of 1 mM Mn2+ Rhodopirellula baltica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
59700
-
 2 * 59700, calculated Rhodopirellula baltica
121900
-
 gel filtration Rhodopirellula baltica

Organism

Organism UniProt Comment Textmining
Rhodopirellula baltica Q7URP0
-
-
Rhodopirellula baltica DSM 10527 Q7URP0
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.57
-
 substrate ADP-mannose, pH 7.5, 25°C Rhodopirellula baltica
1.72
-
 substrate UDP-mannose, pH 7.5, 25°C Rhodopirellula baltica
3.1
-
 substrate GDP-mannose, pH 7.5, 25°C Rhodopirellula baltica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP-mannose + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
 Rhodopirellula baltica ADP + 2-(2-O-(alpha-D-mannopyranosyl)-alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
 ?
ADP-mannose + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
 Rhodopirellula baltica DSM 10527 ADP + 2-(2-O-(alpha-D-mannopyranosyl)-alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
 ?
GDP-mannose + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
 Rhodopirellula baltica GDP + 2-(2-O-(alpha-D-mannopyranosyl)-alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
 ?
GDP-mannose + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
 Rhodopirellula baltica DSM 10527 GDP + 2-(2-O-(alpha-D-mannopyranosyl)-alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
 ?
additional information enzyme is non-specific for the mannosyl-donor, using GDP-mannose, UDP-mannose and ADP-mannose as substrates with decreasing affinity. From the tested substrates only 2-(O-alpha-D-glucopyranosyl)-3-phospho-D-glycerate is used as mannosyl-acceptor. The enzyme is a configuration retaining sugar transferase Rhodopirellula baltica ?
-
 ?
additional information enzyme is non-specific for the mannosyl-donor, using GDP-mannose, UDP-mannose and ADP-mannose as substrates with decreasing affinity. From the tested substrates only 2-(O-alpha-D-glucopyranosyl)-3-phospho-D-glycerate is used as mannosyl-acceptor. The enzyme is a configuration retaining sugar transferase Rhodopirellula baltica DSM 10527 ?
-
 ?
UDP-mannose + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
 Rhodopirellula baltica UDP + 2-(2-O-(alpha-D-mannopyranosyl)-alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
 ?
UDP-mannose + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
 Rhodopirellula baltica DSM 10527 UDP + 2-(2-O-(alpha-D-mannopyranosyl)-alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 59700, calculated Rhodopirellula baltica

Synonyms

Synonyms Comment Organism
MggA
-
 Rhodopirellula baltica
RB5546
-
 Rhodopirellula baltica

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Rhodopirellula baltica

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25
-
 40% of maximum activity Rhodopirellula baltica
90
-
 18% of maximum activity Rhodopirellula baltica

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25
-
 half-life 38.5 h Rhodopirellula baltica
40
-
 half-life 52.8 min Rhodopirellula baltica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Rhodopirellula baltica

pI Value

Organism Comment pI Value Maximum pI Value
Rhodopirellula baltica calculated
-
 5.5