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Literature summary for 2.4.1.27 extracted from

  • Terragni, J.; Bitinaite, J.; Zheng, Y.; Pradhan, S.
    Biochemical characterization of recombinant alpha-glucosyltransferase and analysis of global 5-hydroxymethylcytosine in unique genomes (2012), Biochemistry, 51, 1009-1019.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.1.27

Application

Application Comment Organism
analysis use of recombinant enzyme to estimate global 5-hydroxymethyl cytosine content in a variety of genomic DNAs. Most of the genomic DNAs derived from vertebrate tissue and cell lines contain 5-hydroxymethyl cytosine. DNA from mouse, human, and bovine brains displays 0.5?0.9% of the total nucleotides as 5-hydroxymethyl cytosine, which is higher compared to the levels found in other tissues. A comparison between cancer and healthy tissue genomes suggests a lower percentage of 5-hydroxymethyl cytosine in cancer Tequatrovirus T4
medicine a comparison between cancer and healthy tissue genomes suggests a lower percentage of 5-hydroxymethyl cytosine in cancer Tequatrovirus T4

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Tequatrovirus T4

Inhibitors

Inhibitors Comment Organism Structure
5-hydroxymethyl cytosine beta-D-glucoside containing DNA competitive inhibitor with respect to 5-hydroxymethyl cytosine containing DNA and mixed inhibitor with respect to UDP-glucose Tequatrovirus T4
UDP competitive inhibitor with respect to UDP-glucose and mixed inhibitor with respect to 5-hydroxymethyl cytosine containing DNA Tequatrovirus T4

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00022
-
 DNA containing 24 5-hydroxymethyl cytosine residues pH 7.9, 25°C Tequatrovirus T4
0.00023
-
 DNA containing 12 5-hydroxymethyl cytosine residues pH 7.9, 25°C Tequatrovirus T4
0.0004
-
 5-hydroxymethyl cytosine containing phage T4 DNA pH 7.9, 25°C Tequatrovirus T4
0.0006
-
 DNA containing 6 5-hydroxymethyl cytosine residues pH 7.9, 25°C Tequatrovirus T4
0.003
-
 DNA containing 2 5-hydroxymethyl cytosine residues pH 7.9, 25°C Tequatrovirus T4
0.016
-
 UDP-D-glucose pH 7.9, 25°C Tequatrovirus T4

Organism

Organism UniProt Comment Textmining
Tequatrovirus T4
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation
-
 Tequatrovirus T4
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information enzyme shows strong preference for the glucosylation of DNA containing symmetrical 5-hydroxymethyl cytosine resiudes over hemi-5-hydroxymethyl cytosine. The enzyme is capable of saturation glucosylation on all conformations of 5-hydroxymethyl cytosine.The numbers of 5-hydroxymethyl cytosine residues on DNA can influence catalysis, especially the rate of catalysis, and hemihydroxymethylated DNA is not the preferred substrate of the enzyme Tequatrovirus T4 ?
-
 ?
UDP-D-glucose + 5-hydroxymethyl cytosine containing DNA
-
 Tequatrovirus T4 UDP + 5-hydroxymethyl cytosine beta-D-glucoside containing DNA recombinant enzyme glucosylates 5-hydroxymethyl cytosine containing DNA in a nonprocessive manner, and binding to either 5-hydroxymethyl cytosine containing DNA or uridine diphosphoglucose substrates is random, with both binary complexes being catalytically competent. The numbers of 5-hydroxymethyl cytosine on target sequences influence the turnover numbers for the recombinant enzyme ?
UDP-D-glucose + 5-hydroxymethyl cytosine containing phage T4 DNA
-
 Tequatrovirus T4 UDP + 5-hydroxymethyl cytosine beta-D-glucoside containing phage T4 DNA
-
 ?
UDP-D-glucose + DNA containing 12 5-hydroxymethyl cytosine residues
-
 Tequatrovirus T4 UDP + ?
-
 ?
UDP-D-glucose + DNA containing 2 5-hydroxymethyl cytosine residues
-
 Tequatrovirus T4 UDP + ?
-
 ?
UDP-D-glucose + DNA containing 24 5-hydroxymethyl cytosine residues
-
 Tequatrovirus T4 UDP + ?
-
 ?
UDP-D-glucose + DNA containing 6 5-hydroxymethyl cytosine residues
-
 Tequatrovirus T4 UDP + ?
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.033
-
 DNA containing 12 5-hydroxymethyl cytosine residues pH 7.9, 25°C Tequatrovirus T4
1.283
-
 5-hydroxymethyl cytosine containing phage T4 DNA pH 7.9, 25°C Tequatrovirus T4
1.666
-
 DNA containing 6 5-hydroxymethyl cytosine residues pH 7.9, 25°C Tequatrovirus T4
2.8
-
 DNA containing 2 5-hydroxymethyl cytosine residues pH 7.9, 25°C Tequatrovirus T4
9.66
-
 DNA containing 24 5-hydroxymethyl cytosine residues pH 7.9, 25°C Tequatrovirus T4

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.002
-
 UDP substrate 5-hydroxymethyl cytosine containing DNA, pH 7.9, 25°C Tequatrovirus T4
0.003
-
 5-hydroxymethyl cytosine beta-D-glucoside containing DNA substrate UDP-glucose, pH 7.9, 25°C Tequatrovirus T4
0.004
-
 5-hydroxymethyl cytosine beta-D-glucoside containing DNA substrate 5-hydroxymethyl cytosine containing DNA, pH 7.9, 25°C Tequatrovirus T4
0.009
-
 UDP substrate UDP-glucose, pH 7.9, 25°C Tequatrovirus T4

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
933
-
 DNA containing 2 5-hydroxymethyl cytosine residues pH 7.9, 25°C Tequatrovirus T4
2766
-
 DNA containing 6 5-hydroxymethyl cytosine residues pH 7.9, 25°C Tequatrovirus T4
3200
-
 5-hydroxymethyl cytosine containing phage T4 DNA pH 7.9, 25°C Tequatrovirus T4
4400
-
 DNA containing 24 5-hydroxymethyl cytosine residues pH 7.9, 25°C Tequatrovirus T4
4483
-
 DNA containing 12 5-hydroxymethyl cytosine residues pH 7.9, 25°C Tequatrovirus T4