Any feedback?
Literature summary for 2.4.1.268 extracted from
- Single-step pathway for synthesis of glucosylglycerate in Persephonella marina (2007), J. Bacteriol., 189, 4014-4019.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Persephonella marina |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | - |
Persephonella marina |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.5 | - |
ADP-glucose | pH 6.5, 70°C | Persephonella marina | |
| 2.2 | - |
D-glycerate | pH 6.5, 70°C | Persephonella marina | |
| 6.8 | - |
UDP-glucose | pH 6.5, 70°C | Persephonella marina | |
| 7.1 | - |
GDP-glucose | pH 6.5, 70°C | Persephonella marina | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 20 mM, strongly stimulates glucosylglycerate synthase activity | Persephonella marina | |
| Mg2+ | 20 mM, strongly stimulates glucosylglycerate synthase activity | Persephonella marina | |
| additional information | no stimulation by Ba2+, Co2+, Fe3+, Li+, Mn2+, Sr2+ or Zn2+ | Persephonella marina |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 48000 | - |
4 * 48000, SDS-PAGE | Persephonella marina |
| 48400 | - |
4 * 48400, calculated from sequence | Persephonella marina |
| 210000 | - |
gel filtration | Persephonella marina |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP-glucose + glycerate | Persephonella marina | in Persephonella marina two pathways for synthesis of glucosylglycerate are present: 1. the single-step pathway in with glucosylglycerate synthase (Ggs) catalyzes the synthesis of 2-(alpha-D-glucosyl)-D-glycerate in one-step from ADP-glucose and D-glycerate, and 2. the two-step pathway in which glucosyl-3-phosphoglycerate synthase (GpgS) catalyzes the conversion of NDP-glucose and D-3-phosphoglycerate into glucosyl-3-phosphoglycerate, which is then converted to 2-(alpha-D-glucopyranosyl)-D-glycerate by glucosyl-3-phosphoglycerate phosphatase (GpgP) | 2-O-(alpha-D-glucopyranosyl)-D-glycerate + ADP | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Persephonella marina | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Persephonella marina |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 27.4 | - |
pH 6.5, 70°C, substrate: GDP-glucose | Persephonella marina |
| 47 | - |
pH 6.5, 70°C, substrate: UDP-glucose | Persephonella marina |
| 284.3 | - |
pH 6.5, 70°C, substrate: ADP-glucose | Persephonella marina |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP-glucose + D-glycerate | ADP-glucose is the preferred substrate (0.2843 mol/min*mg), it can be partially replaced by UDP-glucose or GDP-glucose in decreasing order of efficiency (0.047 and 0.0274 mol/min*mg, respectively) | Persephonella marina | 2-O-(alpha-D-glucopyranosyl)-D-glycerate + ADP | - |
? | |
| ADP-glucose + glycerate | in Persephonella marina two pathways for synthesis of glucosylglycerate are present: 1. the single-step pathway in with glucosylglycerate synthase (Ggs) catalyzes the synthesis of 2-(alpha-D-glucosyl)-D-glycerate in one-step from ADP-glucose and D-glycerate, and 2. the two-step pathway in which glucosyl-3-phosphoglycerate synthase (GpgS) catalyzes the conversion of NDP-glucose and D-3-phosphoglycerate into glucosyl-3-phosphoglycerate, which is then converted to 2-(alpha-D-glucopyranosyl)-D-glycerate by glucosyl-3-phosphoglycerate phosphatase (GpgP) | Persephonella marina | 2-O-(alpha-D-glucopyranosyl)-D-glycerate + ADP | - |
? | |
| GDP-alpha-D-glucose + D-glycerate | - |
Persephonella marina | 2-O-(alpha-D-glucopyranosyl)-D-glycerate + GDP | - |
? | |
| additional information | activity is not observed with: ADP-glucose, UDP-galactose, GDP-fucose, GDP-mannose or ADP-ribose. D-Glycerate is the only glucosylacceptor. No activity with mannose-6-phosphate, glucose-1-phosphate, glucose-6-phosphate, trehalose-6-phosphate, and fructose-6-phosphate or with the three-carbon compounds D-3-phosphoglycerate, D-2-phosphoglycerate, D-glycerate, L-glycerate, glycerol, and glycerol-3-phosphate | Persephonella marina | ? | - |
? | |
| UDP-glucose + D-glycerate | - |
Persephonella marina | 2-O-(alpha-D-glucopyranosyl)-D-glycerate + UDP | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 48000, SDS-PAGE | Persephonella marina |
| tetramer | 4 * 48400, calculated from sequence | Persephonella marina |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Ggs | - |
Persephonella marina |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | 85 | - |
Persephonella marina |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | 95 | 70°C: about 65% of maximal activity, 95°C: about 85% of maximal activity | Persephonella marina |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
1 week, no loss of activity | Persephonella marina |
| 85 | - |
half-life: 74 h | Persephonella marina |
| 100 | - |
half-life: 8 min | Persephonella marina |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
- |
Persephonella marina |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 9 | pH 4.5: about 50% of maximal activity, pH 9.0: about 35% of maximal activity | Persephonella marina |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
