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Literature summary for 2.4.1.264 extracted from

  • Barreras, M.; Abdian, P.L.; Ielpi, L.
    Functional characterization of GumK, a membrane-associated beta-glucuronosyltransferase from Xanthomonas campestris required for xanthan polysaccharide synthesis (2004), Glycobiology, 14, 233-241.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Xanthomonas campestris

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
Xanthomonas campestris 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44000
-
x * 44000, SDS-PAGE Xanthomonas campestris

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-glucuronate + D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphosphoundecaprenol Xanthomonas campestris the enzyme is involved in the biosyntesis of xanthan UDP + GlcA-beta-(1->2)-D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphosphoundecaprenol
-
?

Organism

Organism UniProt Comment Textmining
Xanthomonas campestris Q8GCH2
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Xanthomonas campestris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-glucuronate + 6-O-acetyl-D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphosphopolyisoprenol GumK shows diminished activity with 6-O-acetyl-D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphosphopolyisoprenol, a putative intermediate in the synthesis of xanthan. This indicates that acetylation of the internal mannose takes place after the formation of the GumK product Xanthomonas campestris UDP + GlcA-beta-(1->2)-(6-O-acetyl)-D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphosphopolyisoprenol
-
?
UDP-glucuronate + D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphosphopolyisoprenol the enzyme does not show specificity for the lipidic portion of the acceptor. GumK shows diminished activity with 6-O-acetyl-D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphosphopolyisoprenol, a putative intermediate in the synthesis of xanthan. This indicates that acetylation of the internal mannose takes place after the formation of the GumK product Xanthomonas campestris UDP + GlcA-beta-(1->2)-D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphosphopolyisoprenol
-
?
UDP-glucuronate + D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphosphoundecaprenol the enzyme is involved in the biosyntesis of xanthan Xanthomonas campestris UDP + GlcA-beta-(1->2)-D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphosphoundecaprenol
-
?

Subunits

Subunits Comment Organism
? x * 44000, SDS-PAGE Xanthomonas campestris

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20
-
assay at Xanthomonas campestris

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.2
-
assay at Xanthomonas campestris