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Literature summary for 2.4.1.258 extracted from
- The accumulation of Man6GlcNAc2-PP-dolichol in the Saccharomyces cerevisiae deltaalg9 mutant reveals a regulatory role for the Alg3p alpha1,3-Man middle-arm addition in downstream oligosaccharide-lipid and glycoprotein glycan processing (2000), J. Biol. Chem., 275, 4267-4277.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| alg3 encodes enzyme responsible for the alpha-1,3-Man middle-arm addition, MNN1 encoded enzyme adds at least the penultimate alpha-1,3-linked Man of the terminal Manalpha(1-3)Manalpha(1-3)-disaccharide on O-linked glycans | Saccharomyces cerevisiae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dolichyl beta-D-mannosyl phosphate + D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol | Saccharomyces cerevisiae | regulatory role for the Alg3p-dependent alpha1,3-linked Man in subsequent oligosaccharide-lipid and glycoprotein glycan maturation. The presence of this Man appears to provide structural information potentiating the downstream action of the endoplasmic reticulum glucosyltransferases Alg6p, Alg8p and Alg10p, glucosidases Gls1p and Gls2p, and the Golgi Och1p outerchain alpha1,6-Man branch-initiating mannosyltransferase | D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->3)-D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol + dolichyl phosphate | - |
? |  |
| additional information | Saccharomyces cerevisiae | regulatory role for the Alg3p-dependent alpha-1,3-linked Man in subsequent oligosaccharide-lipid and glycoprotein glycan maturation, it provides structural information that potentiates the Alg6p, Alg8p and Alg10p ER glucosyltransferases and Gls1p and Gls2p trimming glucosidases | ? | - |
? | |
| additional information | Saccharomyces cerevisiae A | regulatory role for the Alg3p-dependent alpha-1,3-linked Man in subsequent oligosaccharide-lipid and glycoprotein glycan maturation, it provides structural information that potentiates the Alg6p, Alg8p and Alg10p ER glucosyltransferases and Gls1p and Gls2p trimming glucosidases | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
- |
- |
| Saccharomyces cerevisiae | P38179 | A | - |
| Saccharomyces cerevisiae | P38179 | haploid strain yg414, Alg3p and MNN1 | - |
| Saccharomyces cerevisiae A | P38179 | A | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dolichyl beta-D-mannosyl phosphate + D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol | - |
Saccharomyces cerevisiae | D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->3)-D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol + dolichyl phosphate | - |
? | |
| dolichyl beta-D-mannosyl phosphate + D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol | regulatory role for the Alg3p-dependent alpha1,3-linked Man in subsequent oligosaccharide-lipid and glycoprotein glycan maturation. The presence of this Man appears to provide structural information potentiating the downstream action of the endoplasmic reticulum glucosyltransferases Alg6p, Alg8p and Alg10p, glucosidases Gls1p and Gls2p, and the Golgi Och1p outerchain alpha1,6-Man branch-initiating mannosyltransferase | Saccharomyces cerevisiae | D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->3)-D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol + dolichyl phosphate | - |
? | |
| GDP-alpha-D-mannose + Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)(Manalpha(1-6))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-PP-dolichol | cytosolic alg3 Man5GlcNAc2 precursor | Saccharomyces cerevisiae | GDP + Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)(Manalpha(1-3)Manalpha(1-6))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-PP-dolichol | product of the Alg3p step | ? | |
| GDP-alpha-D-mannose + Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)(Manalpha(1-6))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-PP-dolichol | cytosolic alg3 Man5GlcNAc2 precursor | Saccharomyces cerevisiae A | GDP + Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)(Manalpha(1-3)Manalpha(1-6))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-PP-dolichol | product of the Alg3p step | ? | |
| additional information | MNN1 encoded enzyme adds at least the penultimate alpha-1,3-linked Man of the terminal Manalpha(1-3)Manalpha(1-3)-disaccharide on O-linked glycans | Saccharomyces cerevisiae | ? | - |
? | |
| additional information | regulatory role for the Alg3p-dependent alpha-1,3-linked Man in subsequent oligosaccharide-lipid and glycoprotein glycan maturation, it provides structural information that potentiates the Alg6p, Alg8p and Alg10p ER glucosyltransferases and Gls1p and Gls2p trimming glucosidases | Saccharomyces cerevisiae | ? | - |
? | |
| additional information | MNN1 encoded enzyme adds at least the penultimate alpha-1,3-linked Man of the terminal Manalpha(1-3)Manalpha(1-3)-disaccharide on O-linked glycans | Saccharomyces cerevisiae A | ? | - |
? | |
| additional information | regulatory role for the Alg3p-dependent alpha-1,3-linked Man in subsequent oligosaccharide-lipid and glycoprotein glycan maturation, it provides structural information that potentiates the Alg6p, Alg8p and Alg10p ER glucosyltransferases and Gls1p and Gls2p trimming glucosidases | Saccharomyces cerevisiae A | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Alg3p | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 27 | - |
assay at | Saccharomyces cerevisiae |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | regulatory role for the Alg3p-dependent alpha1,3-linked Man in subsequent oligosaccharide-lipid and glycoprotein glycan maturation. The presence of this Man appears to provide structural information potentiating the downstream action of the endoplasmic reticulum glucosyltransferases Alg6p, Alg8p and Alg10p, glucosidases Gls1p and Gls2p, and the Golgi Och1p outerchain alpha1,6-Man branch-initiating mannosyltransferase | Saccharomyces cerevisiae |
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