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Literature summary for 2.4.1.257 extracted from

  • Ramirez, A.S.; Boilevin, J.; Lin, C.W.; Ha Gan, B.; Janser, D.; Aebi, M.; Darbre, T.; Reymond, J.L.; Locher, K.P.
    Chemo-enzymatic synthesis of lipid-linked GlcNAc2Man5 oligosaccharides using recombinant Alg1, Alg2 and Alg11 proteins (2017), Glycobiology, 27, 726-733 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene ALG2, transient recombinant expression of N-terminally His10-YFP tagged enzyme in HEK-293 cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information chemo-enzymatic synthesis of lipid-linked GlcNAc2Man5 oligosaccharides using recombinant Alg1 (from yeast, EC 2.4.1.142), Alg2, and Alg11 (from yeast, EC 2.4.1.131) proteins, chemo-enzymatic synthesis strategy to extend the glycan moiety of synthetic LLO analogues to Dol-PP-GlcNAc2Man5, method, overview Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum
-
Homo sapiens 5783
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GDP-alpha-D-mannose + alpha-D-Man-(1->3)-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-alpha-D-GlcNAc-diphosphodolichol Homo sapiens
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GDP + alpha-D-Man-(1->3)-[alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-alpha-D-GlcNAc-diphosphodolichol
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9H553
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant N-terminally His10-YFP tagged enzyme from HEK-293 cells by solubilization from membranes with detergent, nickel affinity chromatography, and gel filtration Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GDP-alpha-D-mannose + alpha-D-Man-(1->3)-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-alpha-D-GlcNAc-diphosphodolichol
-
Homo sapiens GDP + alpha-D-Man-(1->3)-[alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-alpha-D-GlcNAc-diphosphodolichol
-
?
additional information chemo-enzymatic synthesis of lipid-linked GlcNAc2Man5 oligosaccharides using recombinant Alg1, Alg2 and Alg11 proteins. Comparison to the reaction of dolichyl-diphosphooligosaccharide-protein glycosyltransferase subunit STT3A (EC 2.4.99.18) Homo sapiens ?
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-

Synonyms

Synonyms Comment Organism
Alg2
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Homo sapiens