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Literature summary for 2.4.1.255 extracted from

  • Rodriguez, A.C.; Yu, S.H.; Li, B.; Zegzouti, H.; Kohler, J.J.
    Enhanced transfer of a photocross-linking N-acetylglucosamine (GlcNAc) analog by an O-GlcNAc transferase mutant with converted substrate specificity (2015), J. Biol. Chem., 290, 22638-22648.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
analysis metabolic labeling method to introduce the diazirine photocross-linking functional group onto O-GlcNAc residues in mammalian cells. Cells are engineered to produce diazirine-modified UDP-GlcNAc (UDP-Glc-NDAz), which is transferred to substrate proteins by endogenous OGT, producing O-GlcNDAz. Modified proteins can be covalently cross-linked to their binding partners, providing information about O-GlcNAc-dependent interactions Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Homo sapiens

Protein Variants

Protein Variants Comment Organism
C917A mutant efficiently transfers diazirine-modified GlcNDAz and has altered substrate specificity, preferring to transfer GlcNDAz rather than GlcNAc to protein substrates Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0021
-
UDP-GlcNAc wild-type, pH 7.2 22°C Homo sapiens
0.0047
-
UDP-N-azidoacetylglucosamine wild-type, pH 7.2 22°C Homo sapiens
0.0085
-
UDP-N-azidoacetylglucosamine mutant C917A, pH 7.2 22°C Homo sapiens
0.0155
-
UDP-GlcNAc mutant C917A, pH 7.2 22°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
casein kinase II + UDP-GlcNAc
-
Homo sapiens ? + UDP
-
?
casein kinase II + UDP-N-azidoacetylglucosamine
-
Homo sapiens ? + UDP
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0015
-
UDP-N-azidoacetylglucosamine wild-type, pH 7.2 22°C Homo sapiens
0.0027
-
UDP-GlcNAc mutant C917A, pH 7.2 22°C Homo sapiens
0.008
-
UDP-GlcNAc wild-type, pH 7.2 22°C Homo sapiens
0.0085
-
UDP-N-azidoacetylglucosamine mutant C917A, pH 7.2 22°C Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.001
-
UDP-N-azidoacetylglucosamine mutant C917A, pH 7.2 22°C Homo sapiens
0.002
-
UDP-GlcNAc mutant C917A, pH 7.2 22°C Homo sapiens
0.003
-
UDP-N-azidoacetylglucosamine wild-type, pH 7.2 22°C Homo sapiens
0.0037
-
UDP-GlcNAc wild-type, pH 7.2 22°C Homo sapiens