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Literature summary for 2.4.1.253 extracted from
- Purification and characterization of UDP-glucuronate: baicalein 7-O-glucuronosyltransferase from Scutellaria baicalensis Georgi. cell suspension cultures (2000), Phytochemistry, 53, 533-538.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid | 0.1 mM, complete inhibition | Scutellaria baicalensis |  |
| CuSO4 | 1 mM, complete inhibition | Scutellaria baicalensis | |
| diethylpyrocarbonate | 0.1 mM, 31% inhibition | Scutellaria baicalensis | |
| EDTA | 5 mM, 17% inhibition | Scutellaria baicalensis | |
| FeCl2 | 1 mM, complete inhibition | Scutellaria baicalensis | |
| MgCl2 | 1 mM, 21.5% inhibition | Scutellaria baicalensis | |
| additional information | N-ethylmaleimide has no effect in activity | Scutellaria baicalensis | |
| p-chloromercuribenzoate | 0.1 mM, complete inhibition | Scutellaria baicalensis | |
| ZnCl2 | 1 mM, complete inhibition | Scutellaria baicalensis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0524 | - |
5,6,7-trihydroxy-2-phenyl-4H-chromen-4-one | pH 7.5, 37°C | Scutellaria baicalensis | |
| 0.0729 | - |
UDP-glucuronate | pH 7.5, 37°C | Scutellaria baicalensis | |
| 0.107 | - |
wogonin | pH 7.5, 37°C | Scutellaria baicalensis | |
| 0.109 | - |
scutellarein | pH 7.5, 37°C | Scutellaria baicalensis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no divalent cation requirement | Scutellaria baicalensis |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 52000 | - |
2 * 52000, does not exist or function as monomer, SDS-PAGE | Scutellaria baicalensis |
| 110000 | - |
gel filtration | Scutellaria baicalensis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-D-glucuronate + 5,6,7-trihydroxyflavone | Scutellaria baicalensis | i.e. 5,6,7-trihydroxy-2-phenyl-4H-chromen-4-one, i.e. baicalein. The enzyme is specific to UDP-glucuronic acid as a sugar donor and flavones with substitution ortho- to the 7-OH group such as baicalein (6-OH), scutellarein (6-OH) and wogonin (8-OMe) | UDP + 5,6,7-trihydroxyflavone-7-O-beta-D-glucuronate | i.e. 5,6-dihydroxy-4-oxo-2-phenyl-4H-chromen-7-yl beta-D-glucopyranosiduronic acid, i.e. baicalein 7-O-glucuronide | ? | |
| UDP-D-glucuronate + 5,6,7-trihydroxyflavone | Scutellaria baicalensis Georgi | i.e. 5,6,7-trihydroxy-2-phenyl-4H-chromen-4-one, i.e. baicalein. The enzyme is specific to UDP-glucuronic acid as a sugar donor and flavones with substitution ortho- to the 7-OH group such as baicalein (6-OH), scutellarein (6-OH) and wogonin (8-OMe) | UDP + 5,6,7-trihydroxyflavone-7-O-beta-D-glucuronate | i.e. 5,6-dihydroxy-4-oxo-2-phenyl-4H-chromen-7-yl beta-D-glucopyranosiduronic acid, i.e. baicalein 7-O-glucuronide | ? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Scutellaria baicalensis | - |
Georgi | - |
| Scutellaria baicalensis Georgi | - |
Georgi | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Scutellaria baicalensis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| callus | callus tissue derived from the petiole of a sterile young plant | Scutellaria baicalensis | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 13.5 | - |
- |
Scutellaria baicalensis |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, stable for more than 2 weeks without loss of activity | Scutellaria baicalensis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | flavonoids lacking a substitution at the position ortho- to the 7-OH (chrysin, apigenin, luteolin, quercetin, formononetin and daidzein) do not serve as substrates | Scutellaria baicalensis | ? | - |
? | |
| additional information | flavonoids lacking a substitution at the position ortho- to the 7-OH (chrysin, apigenin, luteolin, quercetin, formononetin and daidzein) do not serve as substrates | Scutellaria baicalensis Georgi | ? | - |
? | |
| UDP-D-glucuronate + 4',5,6,7-tetrahydroxyflavone | i.e. 5,6,7-trihydroxy-2-(4-hydroxyphenyl)chromen-4-one, i.e. scutellarein. The enzyme is specific to UDP-glucuronic acid as a sugar donor and flavones with substitution ortho- to the 7-OH group such as baicalein (6-OH), scutellarein (6-OH) and wogonin (8-OMe) | Scutellaria baicalensis | UDP + 4',5,6,7-tetrahydroxyflavone 7-O-beta-D-glucuronate | i.e. scutellarein 7-O-glucuronide | ? | |
| UDP-D-glucuronate + 4',5,6,7-tetrahydroxyflavone | i.e. 5,6,7-trihydroxy-2-(4-hydroxyphenyl)chromen-4-one, i.e. scutellarein. The enzyme is specific to UDP-glucuronic acid as a sugar donor and flavones with substitution ortho- to the 7-OH group such as baicalein (6-OH), scutellarein (6-OH) and wogonin (8-OMe) | Scutellaria baicalensis Georgi | UDP + 4',5,6,7-tetrahydroxyflavone 7-O-beta-D-glucuronate | i.e. scutellarein 7-O-glucuronide | ? | |
| UDP-D-glucuronate + 5,6,7-trihydroxyflavone | i.e. 5,6,7-trihydroxy-2-phenyl-4H-chromen-4-one, i.e. baicalein. The enzyme is specific to UDP-glucuronic acid as a sugar donor and flavones with substitution ortho- to the 7-OH group such as baicalein (6-OH), scutellarein (6-OH) and wogonin (8-OMe) | Scutellaria baicalensis | UDP + 5,6,7-trihydroxyflavone-7-O-beta-D-glucuronate | i.e. 5,6-dihydroxy-4-oxo-2-phenyl-4H-chromen-7-yl beta-D-glucopyranosiduronic acid, i.e. baicalein 7-O-glucuronide | ? | |
| UDP-D-glucuronate + 5,6,7-trihydroxyflavone | i.e. 5,6,7-trihydroxy-2-phenyl-4H-chromen-4-one, i.e. baicalein. The enzyme is specific to UDP-glucuronic acid as a sugar donor and flavones with substitution ortho- to the 7-OH group such as baicalein (6-OH), scutellarein (6-OH) and wogonin (8-OMe) | Scutellaria baicalensis | UDP + 5,6,7-trihydroxyflavone-7-O-beta-D-glucuronate | i.e. 5,6-dihydroxy-4-oxo-2-phenyl-4H-chromen-7-yl beta-D-glucopyranosiduronic acid, i.e. baicalein 7-O-glucuronide. Identification and quantitation of the enzyme reaction products are carried out by reversed phase-HPLC | ? | |
| UDP-D-glucuronate + 5,6,7-trihydroxyflavone | i.e. 5,6,7-trihydroxy-2-phenyl-4H-chromen-4-one, i.e. baicalein. The enzyme is specific to UDP-glucuronic acid as a sugar donor and flavones with substitution ortho- to the 7-OH group such as baicalein (6-OH), scutellarein (6-OH) and wogonin (8-OMe) | Scutellaria baicalensis Georgi | UDP + 5,6,7-trihydroxyflavone-7-O-beta-D-glucuronate | i.e. 5,6-dihydroxy-4-oxo-2-phenyl-4H-chromen-7-yl beta-D-glucopyranosiduronic acid, i.e. baicalein 7-O-glucuronide | ? | |
| UDP-D-glucuronate + 5,6,7-trihydroxyflavone | i.e. 5,6,7-trihydroxy-2-phenyl-4H-chromen-4-one, i.e. baicalein. The enzyme is specific to UDP-glucuronic acid as a sugar donor and flavones with substitution ortho- to the 7-OH group such as baicalein (6-OH), scutellarein (6-OH) and wogonin (8-OMe) | Scutellaria baicalensis Georgi | UDP + 5,6,7-trihydroxyflavone-7-O-beta-D-glucuronate | i.e. 5,6-dihydroxy-4-oxo-2-phenyl-4H-chromen-7-yl beta-D-glucopyranosiduronic acid, i.e. baicalein 7-O-glucuronide. Identification and quantitation of the enzyme reaction products are carried out by reversed phase-HPLC | ? | |
| UDP-D-glucuronate + 5,7-dihydroxy-8-methoxyflavone | i.e. 5,7-dihydroxy-8-methoxy-2-phenylchromen-4-one, i.e. wogonin. The enzyme is specific to UDP-glucuronic acid as a sugar donor and flavones with substitution ortho- to the 7-OH group such as baicalein (6-OH), scutellarein (6-OH) and wogonin (8-OMe) | Scutellaria baicalensis | UDP + 5,7-dihydroxy-8-methoxyflavone-7-O-beta-D-glucuronate | i.e wogonin 7-O-glucuronide | ? | |
| UDP-D-glucuronate + 5,7-dihydroxy-8-methoxyflavone | i.e. 5,7-dihydroxy-8-methoxy-2-phenylchromen-4-one, i.e. wogonin. The enzyme is specific to UDP-glucuronic acid as a sugar donor and flavones with substitution ortho- to the 7-OH group such as baicalein (6-OH), scutellarein (6-OH) and wogonin (8-OMe) | Scutellaria baicalensis Georgi | UDP + 5,7-dihydroxy-8-methoxyflavone-7-O-beta-D-glucuronate | i.e wogonin 7-O-glucuronide | ? | |
| UDP-glucuronate + baicalein | - |
Scutellaria baicalensis | UDP + baicalin | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 52000, does not exist or function as monomer, SDS-PAGE | Scutellaria baicalensis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| UBGAT | - |
Scutellaria baicalensis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 40 | - |
Scutellaria baicalensis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 23 | - |
50% of the activity remains after 3 h, 25% of the activity remains after 24 h | Scutellaria baicalensis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
in 50 mM Tris-HCl buffer | Scutellaria baicalensis |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Scutellaria baicalensis | chromatofocusing | - |
4.8 |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Vmax/Km ratio for 5,6,7-trihydroxy-2-phenyl-4H-chromen-4-one (i.e. baicalein) is: 4.9 nkat/mg*micromol, Vmax/Km ratio for scutellarein is 2.67 nkat/mg*micromol, Vmax/Km ratio for wogonin is: 1.71 nkat/mg*micromol | Scutellaria baicalensis |
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