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Literature summary for 2.4.1.251 extracted from

  • Katzen, F.; Ferreiro, D.U.; Oddo, C.G.; Ielmini, M.V.; Becker, A.; Pühler, A.; Ielpi, L.
    Xanthomonas campestris pv. campestris gum mutants: effects on xanthan biosynthesis and plant virulence (1998), J. Bacteriol., 180, 1607-1617.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
synthesis the enzyme is involved in biosynthesis of xanthan, an industrially important exopolysaccharide Xanthomonas campestris

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GDP-mannose + GlcA-beta-(1->2)-D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphospho-dicis,octatrans-undecaprenol Xanthomonas campestris
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GDP + D-Man-beta-(1->4)-GlcA-beta-(1->2)-D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphospho-dicis,octatrans-undecaprenol
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Organism

Organism UniProt Comment Textmining
Xanthomonas campestris
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pv. campestris
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GDP-mannose + GlcA-beta-(1->2)-D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphospho-dicis,octatrans-undecaprenol
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Xanthomonas campestris GDP + D-Man-beta-(1->4)-GlcA-beta-(1->2)-D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphospho-dicis,octatrans-undecaprenol
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Synonyms

Synonyms Comment Organism
gumI
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Xanthomonas campestris

General Information

General Information Comment Organism
malfunction from the biochemical analysis of a defined set of Xanthomonas campestris gum mutants, experimental data are reported for assigning functions to the products of the gum genes. Inactivation of gumK completely abolishes in vitro polymer formation. Permeabilized cells are incubated with UDP-glucose, GDPmannose, and UDP-glucuronic acid, one of them labeled in the sugar moiety. Accumulating intermediates are analysed. Accumulation of GlcA-beta-(1->2)-D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha1-diphospho-(lipid carrier) in the gumI deletion strain. Mutations in gumI have less effect on the amount of in vitro produced polysaccharide. The gumI deletion strain produces about 10% of the polymer amount produced by the wild-type strain Xanthomonas campestris
physiological function the enzyme is involved in biosynthesis of xanthan Xanthomonas campestris