Application | Comment | Organism |
---|---|---|
synthesis | the enzyme is involved in biosynthesis of xanthan, an industrially important exopolysaccharide | Xanthomonas campestris |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP-mannose + GlcA-beta-(1->2)-D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphospho-dicis,octatrans-undecaprenol | Xanthomonas campestris | - |
GDP + D-Man-beta-(1->4)-GlcA-beta-(1->2)-D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphospho-dicis,octatrans-undecaprenol | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Xanthomonas campestris | - |
pv. campestris | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP-mannose + GlcA-beta-(1->2)-D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphospho-dicis,octatrans-undecaprenol | - |
Xanthomonas campestris | GDP + D-Man-beta-(1->4)-GlcA-beta-(1->2)-D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphospho-dicis,octatrans-undecaprenol | - |
? |
Synonyms | Comment | Organism |
---|---|---|
gumI | - |
Xanthomonas campestris |
General Information | Comment | Organism |
---|---|---|
malfunction | from the biochemical analysis of a defined set of Xanthomonas campestris gum mutants, experimental data are reported for assigning functions to the products of the gum genes. Inactivation of gumK completely abolishes in vitro polymer formation. Permeabilized cells are incubated with UDP-glucose, GDPmannose, and UDP-glucuronic acid, one of them labeled in the sugar moiety. Accumulating intermediates are analysed. Accumulation of GlcA-beta-(1->2)-D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha1-diphospho-(lipid carrier) in the gumI deletion strain. Mutations in gumI have less effect on the amount of in vitro produced polysaccharide. The gumI deletion strain produces about 10% of the polymer amount produced by the wild-type strain | Xanthomonas campestris |
physiological function | the enzyme is involved in biosynthesis of xanthan | Xanthomonas campestris |