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Literature summary for 2.4.1.25 extracted from
- Receptor protein of Lysinibacillus sphaericus mosquito-larvicidal toxin displays amylomaltase activity (2018), Insect Biochem. Mol. Biol., 93, 37-46 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21 Star (DE3) cells | Culex quinquefasciatus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | toxic BinA component of the activated binary toxin does not inhibit alpha-glucosidase activity of the enzyme, while BinB reduces the activity by nearly 50% | Culex quinquefasciatus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | Ca2+ provides structural stability to the protein | Culex quinquefasciatus |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 120000 | - |
gel filtration | Culex quinquefasciatus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Culex quinquefasciatus | A0A222C758 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-IDA matrix chromatography | Culex quinquefasciatus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| maltose + glycosyl acceptor | efficient transglycosylation activity | Culex quinquefasciatus | D-glucose | - |
? | |
| maltotriose + glycosyl acceptor | efficient transglycosylation activity | Culex quinquefasciatus | D-glucose + maltooligosaccharides | - |
? | |
| additional information | the enzyme displays weak hydrolytic activity against sucrose and alpha-aryl glucoside and fails to act on trehalose and isomaltose | Culex quinquefasciatus | ? | - |
- |
|
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 62329, calculated from amino acid sequence | Culex quinquefasciatus |
| homodimer | 2 * 62378, MALDI-TOF mass spectrometry | Culex quinquefasciatus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| amylomaltase | - |
Culex quinquefasciatus |
| Cqm1 | the ordered domain (residues 23-560) of surface receptor protein Cqm1 has amylomaltase activity | Culex quinquefasciatus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Culex quinquefasciatus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 51.5 | - |
melting temperature | Culex quinquefasciatus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Culex quinquefasciatus |
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Release 2023.1 (January 2023)
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