Activating Compound | Comment | Organism | Structure |
---|---|---|---|
DMSO | increases the enzyme's hydrolytic activity | Saccharolobus solfataricus |
Cloned (Comment) | Organism |
---|---|
gene treX, cloning from the trehalose biosynthesis gene cluster, expression of the His-tagged enzyme in Escherichia coli | Saccharolobus solfataricus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Saccharolobus solfataricus | the enzyme is involved in glycogen metabolism by selective cleavage of the outer side chain | ? | - |
? | |
additional information | Saccharolobus solfataricus P2 | the enzyme is involved in glycogen metabolism by selective cleavage of the outer side chain | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | - |
gene treX | - |
Saccharolobus solfataricus P2 | - |
gene treX | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme is involved in glycogen metabolism by selective cleavage of the outer side chain | Saccharolobus solfataricus | ? | - |
? | |
additional information | the enzyme exhibits hydrolyzing activity toward alpha-1,6-glycosidic linkages of amylopectin, glycogen, pullulan, and other branched substrates, glycogen is the preferred substrate, TreX shows high specificity for hydrolysis of maltohexaosyl alpha-1,6-beta-cyclodextrin, and high activity in 4-alpha-sulfoxide-glucantransferase activity transferring alpha-1,4-glucan oligosaccharides from one chain to another. The enzyme tetramer shows a 4fold higher catalytic activity than the dimer. The enzyme catalyzes intramolecular transglycosylation of maltooligosacchrides, i.e. disproportionation to produce linear alpha-1,4-glucans, as well as intramolecular transglycosylation of glycogen | Saccharolobus solfataricus | ? | - |
? | |
additional information | the enzyme is involved in glycogen metabolism by selective cleavage of the outer side chain | Saccharolobus solfataricus P2 | ? | - |
? | |
additional information | the enzyme exhibits hydrolyzing activity toward alpha-1,6-glycosidic linkages of amylopectin, glycogen, pullulan, and other branched substrates, glycogen is the preferred substrate, TreX shows high specificity for hydrolysis of maltohexaosyl alpha-1,6-beta-cyclodextrin, and high activity in 4-alpha-sulfoxide-glucantransferase activity transferring alpha-1,4-glucan oligosaccharides from one chain to another. The enzyme tetramer shows a 4fold higher catalytic activity than the dimer. The enzyme catalyzes intramolecular transglycosylation of maltooligosacchrides, i.e. disproportionation to produce linear alpha-1,4-glucans, as well as intramolecular transglycosylation of glycogen | Saccharolobus solfataricus P2 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | at pH 7.0, gel filtration and analytical sedimentation ultracentrifugation | Saccharolobus solfataricus |
dimer or tetramer | at pH 5.5, gel filtration and analytical sedimentation ultracentrifugation | Saccharolobus solfataricus |
More | the tetramer shows a 4fold higher catalytic activity than the dimer | Saccharolobus solfataricus |
tetramer | at pH 5.5-6.5 in presence of DMSO, gel filtration and analytical sedimentation ultracentrifugation | Saccharolobus solfataricus |
Synonyms | Comment | Organism |
---|---|---|
debranching enzyme | - |
Saccharolobus solfataricus |
TreX | - |
Saccharolobus solfataricus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | - |
- |
Saccharolobus solfataricus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
- |
Saccharolobus solfataricus |