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Literature summary for 2.4.1.25 extracted from
- Enzymatic synthesis of dimaltosyl-beta-cyclodextrin via a transglycosylation reaction using TreX, a Sulfolobus solfataricus P2 debranching enzyme (2008), Biochem. Biophys. Res. Commun., 366, 98-103.
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | the enzyme is useful in the enzymatic synthesis of dimaltosyl-beta-cyclodextrin via a transglycosylation reaction | Saccharolobus solfataricus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of His-tagged enzyme in Escherichia coli strain MC1061 | Saccharolobus solfataricus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| beta-cyclodextrin | a mixed-type inhibitor | Saccharolobus solfataricus |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.591 | - |
maltosyl-beta-cyclodextrin | pH 5.5, 75°C | Saccharolobus solfataricus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharolobus solfataricus | - |
gene treX | - |
| Saccharolobus solfataricus P2 | - |
gene treX | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli strain MC1061 by nickel affinity chromatography | Saccharolobus solfataricus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| maltose + beta-cyclodextrin | condensation reaction mechanism, overview | Saccharolobus solfataricus | 6-O-alpha-maltosyl-beta-cyclodextrin | mass spectrometric product identification | ? | |
| maltose + beta-cyclodextrin | condensation reaction mechanism, overview | Saccharolobus solfataricus P2 | 6-O-alpha-maltosyl-beta-cyclodextrin | mass spectrometric product identification | ? | |
| maltose + maltosyl-beta-cyclodextrin | - |
Saccharolobus solfataricus | ? | - |
? | |
| maltose + maltosyl-beta-cyclodextrin | - |
Saccharolobus solfataricus P2 | ? | - |
? | |
| additional information | synthesis of di-O-alpha-maltosyl-beta-cyclodextrin from 6-O-alpha-maltosyl-beta-cyclodextrin via a transglycosylation reaction, TreX transfers the maltosyl residue of a G2-beta-cyclodextrin to another molecule of G2-beta-cyclodextrin by forming an alpha-1,6-glucosidic linkage. TreX shows specificity for a branched glucosyl chain bigger than DP2 and no activity toward glucosyl-beta-cyclodextrin, transglycosylation reaction mechanism, overview | Saccharolobus solfataricus | ? | - |
? | |
| additional information | synthesis of di-O-alpha-maltosyl-beta-cyclodextrin from 6-O-alpha-maltosyl-beta-cyclodextrin via a transglycosylation reaction, TreX transfers the maltosyl residue of a G2-beta-cyclodextrin to another molecule of G2-beta-cyclodextrin by forming an alpha-1,6-glucosidic linkage. TreX shows specificity for a branched glucosyl chain bigger than DP2 and no activity toward glucosyl-beta-cyclodextrin, transglycosylation reaction mechanism, overview | Saccharolobus solfataricus P2 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| debranching enzyme | - |
Saccharolobus solfataricus |
| TreX | - |
Saccharolobus solfataricus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | 75 | assay at | Saccharolobus solfataricus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0113 | - |
maltosyl-beta-cyclodextrin | pH 5.5, 75°C | Saccharolobus solfataricus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
assay at | Saccharolobus solfataricus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0556 | - |
beta-cyclodextrin | pH 5.5, 75°C, recombinant His-tagged enzyme | Saccharolobus solfataricus | |
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