Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Aquifex aeolicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.2 | - |
maltopentaose | pH 6.6, 70°C | Aquifex aeolicus | |
4.5 | - |
maltotetraose | pH 6.6, 70°C | Aquifex aeolicus | |
4.5 | - |
maltohexaose | pH 6.6, 70°C | Aquifex aeolicus | |
5.7 | - |
maltoheptaose | pH 6.6, 70°C | Aquifex aeolicus | |
6.1 | - |
maltotriose | pH 6.6, 70°C | Aquifex aeolicus | |
71 | - |
maltose | pH 6.6, 70°C | Aquifex aeolicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aquifex aeolicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Aquifex aeolicus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
44.2 | - |
- |
Aquifex aeolicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
amylose | the enzyme produces a cycloamylose with a minimum degree of polymerization of 16 | Aquifex aeolicus | cycloamylose | - |
? | |
maltoheptaose + maltoheptaose | - |
Aquifex aeolicus | maltooligosaccharides | - |
? | |
maltohexaose + maltohexaose | - |
Aquifex aeolicus | maltooligosaccharides | - |
? | |
maltopentaose + maltopentaose | - |
Aquifex aeolicus | maltooligosaccharides | - |
? | |
maltose + maltose | - |
Aquifex aeolicus | maltooligosaccharides | - |
? | |
maltotetraose + maltotetraose | - |
Aquifex aeolicus | maltooligosaccharides | - |
? | |
maltotriose + maltotriose | - |
Aquifex aeolicus | maltooligosaccharides | - |
? | |
additional information | the smallest acceptor is glucose, the smallest donor is maltose | Aquifex aeolicus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
4-alpha-glucanotransferase | - |
Aquifex aeolicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
- |
Aquifex aeolicus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | 100 | 60°C: about 50% of maximal activity, 100°C: about 30% of maximal activity | Aquifex aeolicus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
30 min, less than 10% loss of activity | Aquifex aeolicus |
90 | - |
30 min, about 30% loss of activity | Aquifex aeolicus |
100 | - |
30 min, about 90% loss of activity | Aquifex aeolicus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.3 | - |
maltose | pH 6.6, 70°C | Aquifex aeolicus | |
16 | - |
maltoheptaose | pH 6.6, 70°C | Aquifex aeolicus | |
17 | - |
maltotetraose | pH 6.6, 70°C | Aquifex aeolicus | |
18 | - |
maltotriose | pH 6.6, 70°C | Aquifex aeolicus | |
22 | - |
maltopentaose | pH 6.6, 70°C | Aquifex aeolicus | |
22 | - |
maltohexaose | pH 6.6, 70°C | Aquifex aeolicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Aquifex aeolicus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | 11 | stable | Aquifex aeolicus |