Crystallization (Comment) | Organism |
---|---|
crystallized in 2 forms, I and II, form I crystals belongs to hexagonal space group P6(4)22, form II crystals to orthorhombic space group P2(1)2(1)2 | Thermococcus litoralis |
hanging drop vapor diffusion method at 25°C, crystal structure of the enzyme with and without an inhibitor, acarbose. The acarbose-bound structure clarifies that Glu123 and Asp214 are the catalytic nucleophile and acid/base catalyst, respectively | Thermococcus litoralis |
Protein Variants | Comment | Organism |
---|---|---|
D214N | the specific activity of the D214N mutant is decreased about 10000fold as compared with that of the wild-type enzyme | Thermococcus litoralis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acarbose | - |
Thermococcus litoralis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
78000 | - |
2 * 78000, calculated from sequence | Thermococcus litoralis |
168000 | - |
gel filtration | Thermococcus litoralis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Dictyoglomus thermophilum | - |
- |
- |
Pyrococcus furiosus | - |
- |
- |
Solanum tuberosum | - |
- |
- |
Thermococcus kodakarensis | - |
- |
- |
Thermococcus litoralis | - |
- |
- |
Thermococcus litoralis | O32462 | - |
- |
Thermococcus litoralis DSM 5473 | O32462 | - |
- |
Thermus aquaticus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Thermococcus litoralis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
amylose + maltopentaose | - |
Thermococcus kodakarensis | cyclic alpha-1,4-glucan | - |
? | |
amylose + maltopentaose | - |
Thermus aquaticus | cyclic alpha-1,4-glucan | cycloamylose | ? | |
amylose + maltopentaose | - |
Solanum tuberosum | cyclic alpha-1,4-glucan | cycloamylose | ? | |
amylose + maltopentaose | - |
Thermococcus litoralis | cyclic alpha-1,4-glucan | cycloamylose | ? | |
maltose + maltose | key role in maltose metabolism | Thermococcus litoralis | maltotriose + glucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 78000, calculated from sequence | Thermococcus litoralis |
Synonyms | Comment | Organism |
---|---|---|
TLGT | - |
Thermococcus litoralis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
assay at | Thermococcus litoralis |