Application | Comment | Organism |
---|---|---|
medicine | understanding of the molecular basis of Cori's disease | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
cloned into pTrc99A plasmid vector and expressed in Escherichia coli JM105 | Saccharomyces cerevisiae |
human glycogen debrancher gene assigned to chromosome 1p21, cloned and expressed in insect cells | Homo sapiens |
molecular cloning, sequencing and analysis of cDNA | Oryctolagus cuniculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.8 | - |
phi-dextrin | recombinant enzyme | Saccharomyces cerevisiae | |
3 | - |
phi-dextrin | native enzyme | Saccharomyces cerevisiae | |
10.5 | - |
6-O-alpha-D-glucosyl-cyclomaltoheptaose | native enzyme | Saccharomyces cerevisiae | |
11.2 | - |
6-O-alpha-D-glucosyl-cyclomaltoheptaose | recombinant enzyme | Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
165000 | - |
purified recombinant enzyme, SDS-PAGE | Saccharomyces cerevisiae |
174900 | - |
estimated from amino acid sequence, deduced from nucleotide sequence | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Gallus gallus | - |
chicken | - |
Homo sapiens | - |
human | - |
Oryctolagus cuniculus | - |
rabbit | - |
Saccharomyces cerevisiae | - |
yeast | - |
Squalus acanthias | - |
dogfish | - |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
- |
Oryctolagus cuniculus |
native and recombinant enzyme | Saccharomyces cerevisiae |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Homo sapiens | - |
muscle | skeletal muscle | Gallus gallus | - |
muscle | skeletal muscle | Homo sapiens | - |
muscle | skeletal muscle | Oryctolagus cuniculus | - |
muscle | skeletal muscle | Squalus acanthias | - |
muscle | pectoralis muscle | Gallus gallus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4.91 | - |
- |
Saccharomyces cerevisiae |
Storage Stability | Organism |
---|---|
-80°C, purified recombinant enzyme, 20 mM sodium phosphate buffer containing 15% glycerol, pH 7.0, no decrease in acivity observed for 1 month | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
6-O-alpha-D-glucosyl-cyclomaltoheptaose + H2O | - |
Saccharomyces cerevisiae | D-glucose + cyclodextrins | - |
? | |
additional information | - |
Escherichia coli | ? | - |
? | |
additional information | 2 different enzyme activities, oligo-1,4-1,4-glucan-4-glycosyltransferase EC 2.4.1.25 and amylo-1,6-glucosidase EC 3.2.1.33 reside on the same polypeptide chain | Saccharomyces cerevisiae | ? | - |
? | |
additional information | 2 different enzyme activities, oligo-1,4-1,4-glucan-4-glycosyltransferase EC 2.4.1.25 and amylo-1,6-glucosidase EC 3.2.1.33 reside on the same polypeptide chain | Oryctolagus cuniculus | ? | - |
? | |
phi-dextrin | - |
Saccharomyces cerevisiae | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GDE | - |
Saccharomyces cerevisiae |
glycogen debranching enzyme | - |
Saccharomyces cerevisiae |
oligo-1,4-1,4-glucantransferase | - |
Saccharomyces cerevisiae |
oligo-1,4-1,4-glucantransferase | - |
Oryctolagus cuniculus |