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Literature summary for 2.4.1.25 extracted from
- Disproportionating enzyme (4-alpha-glucanotransferase; EC 2.4.1.25) of potato. Purification, molecular cloning, and potential role in starch metabolism (1993), J. Biol. Chem., 268, 1391-1396.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| cDNA clone isolated and expressed in Escherichia coli | Solanum tuberosum |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| amyloplast | - |
Solanum tuberosum | 9501 | - |
| plastid | - |
Solanum tuberosum | 9536 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 59500 | - |
native enzyme, SDS-PAGE | Solanum tuberosum |
| 60000 | - |
SDS-PAGE | Solanum tuberosum |
| 64950 | - |
estimated from amino acid sequence, deduced from nucleotide sequence | Solanum tuberosum |
| 65000 | - |
precursor, SDS-PAGE | Solanum tuberosum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| maltodextrin + maltose | Solanum tuberosum | - |
maltooligosaccharides | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Solanum tuberosum | Q06801 | potato | - |
| Solanum tuberosum | Q06801 | L.cv. May Queen | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Solanum tuberosum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Solanum tuberosum | - |
| root | - |
Solanum tuberosum | - |
| stem | - |
Solanum tuberosum | - |
| stolon | - |
Solanum tuberosum | - |
| tuber | - |
Solanum tuberosum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 47.5 | - |
- |
Solanum tuberosum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1,4-alpha-D-glucan + 1,4-alpha-D-glucan | - |
Solanum tuberosum | maltooligosaccharides | - |
? | |
| 1,4-alpha-D-glucan + glucose | - |
Solanum tuberosum | maltooligosaccharides | - |
? | |
| amylopectin + D-glucose | - |
Solanum tuberosum | small oligosaccharides | without maltose | ? | |
| amylopectin + maltopentaose | - |
Solanum tuberosum | maltooligosaccharides | - |
? | |
| amylose + D-glucose | - |
Solanum tuberosum | low molecular mass oligosaccharides | - |
? | |
| amylose + maltopentaose | - |
Solanum tuberosum | maltooligosaccharides | - |
? | |
| amylose + maltopentaose | - |
Solanum tuberosum | cyclic alpha-1,4-glucan | - |
? | |
| maltodextrin + maltose | - |
Solanum tuberosum | maltooligosaccharides | - |
? | |
| maltopentaose + maltotriose | - |
Solanum tuberosum | D-glucose + maltooligosaccharides | - |
? | |
| maltotetraose + maltopentaose | - |
Solanum tuberosum | maltooligosaccharides | - |
? | |
| maltotetraose + maltotriose | - |
Solanum tuberosum | maltodextrins | - |
? | |
| maltotetraose + maltotriose | - |
Solanum tuberosum | D-glucose + maltooligosaccharides | - |
? | |
| additional information | maltose is not a product | Solanum tuberosum | ? | - |
? | |
| additional information | maltose is no substrate | Solanum tuberosum | ? | - |
? | |
| starch + D-glucose | - |
Solanum tuberosum | low molecular mass oligosaccharides | - |
? | |
| starch + maltopentaose | - |
Solanum tuberosum | alpha-1,4-D-glucans | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 60000, SDS-PAGE | Solanum tuberosum |
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