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Literature summary for 2.4.1.25 extracted from

  • Schmidt, J.; John, M.
    Starch metabolism in Pseudomonas stutzeri. II. Purification and properties of a dextrin glycosyl-transferase (D-enzyme) and amylomaltase (1979), Biochim. Biophys. Acta, 566, 100-114.
    View publication on PubMed
Search for more literature for 2.4.1.25

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol D-enzyme, slight inhibition Pseudomonas stutzeri
cyclomaltohexaose inhibition 25% Pseudomonas stutzeri
dithiothreitol D-enzyme, slight inhibition Pseudomonas stutzeri
EDTA D-enzyme, slight inhibition Pseudomonas stutzeri
Fe2+ D-enzyme, 50% inhibition Pseudomonas stutzeri
Hg2+ D-enzyme Pseudomonas stutzeri
methyl-alpha-D-glucoside competitive inhibition Escherichia coli
p-chloromercuribenzoic acid D-enzyme, 30% inhibition Pseudomonas stutzeri
phenyl-beta-D-glucoside competitive inhibition Escherichia coli
Tris D-enzyme, 50 mM, 35% inhibition Pseudomonas stutzeri
Zn2+ D-enzyme Pseudomonas stutzeri

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
7.1
-
 maltotetraose
-
 Pseudomonas stutzeri
8.3
-
 maltose D-enzyme Pseudomonas stutzeri

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000
-
 1 * 38000 + 1 * 47000, amylomaltase Pseudomonas stutzeri
47000
-
 1 * 38000 + 1 * 47000, amylomaltase Pseudomonas stutzeri
52000
-
 1 * 52000 + 1 * 63000, D-enzyme Pseudomonas stutzeri
63000
-
 1 * 52000 + 1 * 63000, D-enzyme Pseudomonas stutzeri
71000
-
-
 Escherichia coli
74000
-
 amylomaltase, gel filtration Pseudomonas stutzeri
115000
-
 D-enzyme, gel filtration Pseudomonas stutzeri

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-
Escherichia coli
-
-
-
Escherichia coli ML 30
-
-
-
Pseudomonas stutzeri
-
 NRRL B3389
-
Pseudomonas stutzeri NRRL B3389
-
 NRRL B3389
-
Solanum tuberosum
-
 potato
-
Streptococcus sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
both amylomaltase and D-enzyme Pseudomonas stutzeri
E. coli ML 30, partially Escherichia coli

Storage Stability

Storage Stability Organism
4°C, stored as a suspension in 3.2 M ammonium sulfate, no significant loss of activity for at least 1 month Pseudomonas stutzeri

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
maltoheptaose + maltotriose D-enzyme Pseudomonas stutzeri maltononaose + D-glucose
-
 ?
maltohexaose + maltodextrin only amylomaltase, no polymers larger than the initial maltodextrin substrate, maltohexaose is a good donor substrate, but unable to function as an acceptor Pseudomonas stutzeri D-glucose + maltopentaose
-
 ?
maltononaose + maltotriose D-enzyme Pseudomonas stutzeri maltoundecaose + D-glucose
-
 ?
maltopentaose + maltodextrin
-
 Pseudomonas stutzeri maltose + higher dextrins
-
 ?
maltopentaose + maltotriose D-enzyme Pseudomonas stutzeri maltoheptaose + D-glucose
-
 ?
maltopentaose + maltotriose amylomaltose Pseudomonas stutzeri homologous 1,4-alpha-D-glucans
-
 ?
maltose + maltose
-
 Escherichia coli maltotriose + glucose
-
 ?
maltose + maltose D-enzyme Pseudomonas stutzeri maltotriose + glucose
-
 ?
maltose + maltose D-enzyme Pseudomonas stutzeri NRRL B3389 maltotriose + glucose
-
 ?
maltose + maltose
-
 Escherichia coli ML 30 maltotriose + glucose
-
 ?
maltotetraose + maltodextrin
-
 Pseudomonas stutzeri maltose + higher dextrins
-
 ?
maltotetraose + maltotriose D-enzyme Pseudomonas stutzeri glucose + maltoheptaose + maltodecaose
-
 ?
maltotetraose + maltotriose amylomaltose Pseudomonas stutzeri homologous 1,4-alpha-glucans
-
 ?
maltotetraose + maltotriose
-
 Solanum tuberosum D-glucose + maltooligosaccharides
-
 ?
maltotetraose + maltotriose amylomaltose, most active with maltotetraose Pseudomonas stutzeri D-glucose + maltooligosaccharides
-
 ?
maltotriose + maltodextrin
-
 Pseudomonas stutzeri maltose + higher dextrins
-
 ?
maltotriose + maltodextrin
-
 Pseudomonas stutzeri NRRL B3389 maltose + higher dextrins
-
 ?
maltotriose + maltodextrin amylomaltase Pseudomonas stutzeri maltopentaose + ?
-
 ?
maltotriose + maltodextrin amylomaltase Pseudomonas stutzeri NRRL B3389 maltopentaose + ?
-
 ?
maltotriose + maltotriose D-enzyme Pseudomonas stutzeri maltopentaose + D-glucose
-
 ?
maltotriose + maltotriose D-enzyme Pseudomonas stutzeri D-glucose + maltopentaose + maltoheptaose + maltononaose + maltoundecaose
-
 ?
maltotriose + maltotriose amylomaltose Pseudomonas stutzeri homologous alpha-1,4-D-glucans
-
 ?
additional information
-
 Escherichia coli ?
-
 ?
additional information maltose is not a donor substrate, only weak acceptor activity Solanum tuberosum ?
-
 ?
additional information D-enzyme, maltohexaose is no initial substrate Pseudomonas stutzeri ?
-
 ?
additional information 2 glycosyltransferases, amylomaltase and D-enzyme Pseudomonas stutzeri ?
-
 ?
additional information D-enzyme, maltohexaose is no initial substrate Pseudomonas stutzeri NRRL B3389 ?
-
 ?
additional information 2 glycosyltransferases, amylomaltase and D-enzyme Pseudomonas stutzeri NRRL B3389 ?
-
 ?
additional information
-
 Escherichia coli ML 30 ?
-
 ?

Subunits

Subunits Comment Organism
dimer 1 * 38000 + 1 * 47000, amylomaltase Pseudomonas stutzeri
dimer 1 * 52000 + 1 * 63000, D-enzyme Pseudomonas stutzeri

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 D-enzyme Pseudomonas stutzeri
37
-
 amylomaltase Pseudomonas stutzeri

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37 42 D-enzyme, activity decreases rapidly above 37°C and is almost completely lost at 42°C Pseudomonas stutzeri
50
-
 amylomaltase, rapidly inactivated at temperatures above Pseudomonas stutzeri

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.9
-
-
 Escherichia coli
7.6 7.7 both amylomaltase and D-enzyme Pseudomonas stutzeri